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The map label for this gene is evgA

Identifier: 218696011

GI number: 218696011

Start: 2716136

End: 2716750

Strand: Direct

Name: evgA

Synonym: EC55989_2662

Alternate gene names: 218696011

Gene position: 2716136-2716750 (Clockwise)

Preceding gene: 218696008

Following gene: 218696012

Centisome position: 52.69

GC content: 40.33

Gene sequence:

>615_bases
ATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAAT
CTTAGCGGAGTTGACTGAAGGGGGAAGCGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCG
ATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATTGTCTCC
GCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGATTCGTGAGTAAAAAAGAAGGCATGAA
CAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTT
TAACGTCCGACCAGCAAAAACTCGACTCCTTATCGAAACAAGAAATTAGTGTCATGCGGTATATTCTTGATGGCAAGGAT
AATAATGACATTGCTGAAAAAATGTTCATCAGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGA
ATGTAAATCACTGATGGATCTTTACACATTCGCGCAACGTAACAAAATCGGCTAA

Upstream 100 bases:

>100_bases
AGCTCAGATTTTCTTATTTTTATTGAGAAAATGAGATGACGCCTTATGTCTGTATTACTACAGGGAGAAGGGAGATGCTT
CATTGCAAAGGGAATAATCT

Downstream 100 bases:

>100_bases
TCACATGAAGTTTTTACCCTATTTTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACA
TCGAATATCGTGGCATCAGT

Product: DNA-binding transcriptional activator EvgA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVS
AKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG

Sequences:

>Translated_204_residues
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVS
AKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG
>Mature_204_residues
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVS
AKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG

Specific function: Member of the two-component regulatory system evgS/evgA. Regulates the expression of emrKY operon and yfdX. Seems also to control expression of at least one other multidrug efflux operon

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=100, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1786747, Length=201, Percent_Identity=32.3383084577114, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788222, Length=207, Percent_Identity=27.536231884058, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788521, Length=194, Percent_Identity=28.8659793814433, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790102, Length=183, Percent_Identity=28.9617486338798, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787473, Length=181, Percent_Identity=24.8618784530387, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788546, Length=190, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EVGA_ECO57 (P0ACZ6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   H91034
- RefSeq:   NP_288939.1
- RefSeq:   NP_311275.1
- ProteinModelPortal:   P0ACZ6
- SMR:   P0ACZ6
- EnsemblBacteria:   EBESCT00000028630
- EnsemblBacteria:   EBESCT00000057600
- GeneID:   915651
- GeneID:   958096
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3631
- KEGG:   ecs:ECs3248
- GeneTree:   EBGT00050000008652
- HOGENOM:   HBG753323
- OMA:   NGYSYFP
- ProtClustDB:   PRK09958
- BioCyc:   ECOL83334:ECS3248-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00038
- SMART:   SM00421
- SMART:   SM00448

Pfam domain/function: PF00196 GerE; PF00072 Response_reg; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 22691; Mature: 22691

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00622 HTH_LUXR_1; PS50043 HTH_LUXR_2; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNG
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCH
IQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGY
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCE
CYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKDNNDIAEKMFISNKTVSTYKS
EEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
RLMEKLECKSLMDLYTFAQRNKIG
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNG
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCH
IQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGY
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCE
CYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKDNNDIAEKMFISNKTVSTYKS
EEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
RLMEKLECKSLMDLYTFAQRNKIG
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796