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The map label for this gene is yfcG

Identifier: 218695901

GI number: 218695901

Start: 2618220

End: 2618867

Strand: Direct

Name: yfcG

Synonym: EC55989_2546

Alternate gene names: 218695901

Gene position: 2618220-2618867 (Clockwise)

Preceding gene: 218695897

Following gene: 218695902

Centisome position: 50.79

GC content: 51.23

Gene sequence:

>648_bases
ATGATCGATCTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTTGATTATCG
CTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGG
CAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGTTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCT
GAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGG
ACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATC
AGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGC
ATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTCGCAATGTATCCGGCAGTCAA
GAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGG
ATAGTTAA

Upstream 100 bases:

>100_bases
CTATAGAGCCTTTTTGTCACTGTCACGTGACGAATACTCACGTAAGTCGCCGCGCTTTCTATACTTGTGGTGTTCAGATG
ATTAAACAACCGGAGCTGCA

Downstream 100 bases:

>100_bases
CAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATT
CGTATAAAGAACCTTCGTTT

Product: glutathione S-transferase

Products: NA

Alternate protein names: GST-like protein yfcG

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFLRISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLA
EKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS

Sequences:

>Translated_215_residues
MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFLRISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLA
EKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS
>Mature_215_residues
MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFLRISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLA
EKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro)

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain

Homologues:

Organism=Escherichia coli, GI1788640, Length=215, Percent_Identity=100, Blast_Score=446, Evalue=1e-127,
Organism=Escherichia coli, GI87082195, Length=214, Percent_Identity=41.1214953271028, Blast_Score=153, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324100, Length=233, Percent_Identity=33.9055793991416, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571670, Length=164, Percent_Identity=28.0487804878049, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864598, Length=207, Percent_Identity=28.5024154589372, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17864592, Length=201, Percent_Identity=25.8706467661692, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI85725204, Length=203, Percent_Identity=27.5862068965517, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17737923, Length=203, Percent_Identity=27.5862068965517, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646249, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17864594, Length=195, Percent_Identity=25.1282051282051, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI45549270, Length=201, Percent_Identity=24.8756218905473, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFCG_ECOLI (P77526)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D65002
- RefSeq:   AP_002902.1
- RefSeq:   NP_416805.1
- PDB:   3GX0
- PDBsum:   3GX0
- ProteinModelPortal:   P77526
- SMR:   P77526
- STRING:   P77526
- EnsemblBacteria:   EBESCT00000003625
- EnsemblBacteria:   EBESCT00000017071
- GeneID:   946763
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2299
- KEGG:   eco:b2302
- EchoBASE:   EB3863
- EcoGene:   EG14110
- eggNOG:   COG0625
- GeneTree:   EBGT00050000009210
- HOGENOM:   HBG753188
- OMA:   RYVNETN
- ProtClustDB:   PRK13972
- BioCyc:   EcoCyc:G7194-MONOMER
- BioCyc:   MetaCyc:G7194-MONOMER
- Genevestigator:   P77526
- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:1.20.1050.10
- Gene3D:   G3DSA:3.40.30.10

Pfam domain/function: PF00043 GST_C; PF02798 GST_N; SSF47616 GST_C_like; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 24516; Mature: 24516

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50405 GST_CTER; PS50404 GST_NTER

Important sites: BINDING 11-11 BINDING 38-38 BINDING 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFLRISPNNKIPAIVDHSP
CEEEEECCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCCCEEEECCC
ADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNH
CCCCCCHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYSIADIACWPWVNAWTRQRIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHCCCEE
AMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS
EECHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFLRISPNNKIPAIVDHSP
CEEEEECCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCCCEEEECCC
ADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNH
CCCCCCHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYSIADIACWPWVNAWTRQRIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHCCCEE
AMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS
EECHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503