Click here to switch to the map view.

The map label for this gene is yfaO

Identifier: 218695853

GI number: 218695853

Start: 2566577

End: 2567002

Strand: Direct

Name: yfaO

Synonym: EC55989_2497

Alternate gene names: 218695853

Gene position: 2566577-2567002 (Clockwise)

Preceding gene: 218695850

Following gene: 218695855

Centisome position: 49.79

GC content: 46.71

Gene sequence:

>426_bases
ATGCGACAACGGACTATTGTATGCCCTTTGATTCAAAATGATGGTGCTTATTTGCTGTGTAAAATGGCCGACGATCGCGG
CGTTTTCCCCGGTCAATGGGCGCTTTCGGGTGGCGGCGTGGAGTCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAA
TTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACG
TATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTGACTGCGTTTCTGCCAACCGAGAAGTGAAAAT
AAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTAAATGTTGCCACCCGAAAAA
CGTTACGTTTGAAAGGTCTTCTTTAA

Upstream 100 bases:

>100_bases
ATAAGTGCAGTTATCATTGCAAAAGAGATTACTGTCACTTTCACATATTTCTGAGTTACCGTATTCTCATGGTTTCTTTT
TATTTATAAGGAATACCCCT

Downstream 100 bases:

>100_bases
CAATGATGACAGCCATCACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTT
TTCCACATGCATGACTAAAC

Product: putative NUDIX hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEEALRREIREELGEQLLLTEITPWTFSDDIRTKT
YADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL

Sequences:

>Translated_141_residues
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEEALRREIREELGEQLLLTEITPWTFSDDIRTKT
YADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL
>Mature_141_residues
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEEALRREIREELGEQLLLTEITPWTFSDDIRTKT
YADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL

Specific function: Catalyzes the hydrolysis of nucleoside triphosphates, with a preference for pyrimidine deoxynucleoside triphosphates (dUTP, dTTP and dCTP)

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI1788585, Length=141, Percent_Identity=98.5815602836879, Blast_Score=287, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUDI_ECO24 (A7ZP69)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463598.1
- ProteinModelPortal:   A7ZP69
- SMR:   A7ZP69
- STRING:   A7ZP69
- EnsemblBacteria:   EBESCT00000022902
- GeneID:   5590356
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2546
- eggNOG:   COG0494
- GeneTree:   EBGT00050000009528
- HOGENOM:   HBG741910
- OMA:   LTHIAPW
- ProtClustDB:   PRK15472
- BioCyc:   ECOL331111:ECE24377A_2546-MONOMER
- HAMAP:   MF_01846
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- Gene3D:   G3DSA:3.90.79.10
- PRINTS:   PR00502

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: 3.6.-.- [C]

Molecular weight: Translated: 16361; Mature: 16361

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEEALRREIREELGE
CCCCEEEEEEEECCCCEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCC
QLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPE
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEHHCCCCEEEECCCCCCCCCCCCC
DLVHYDLNVATRKTLRLKGLL
CEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEEALRREIREELGE
CCCCEEEEEEEECCCCEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCC
QLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPE
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEHHCCCCEEEECCCCCCCCCCCCC
DLVHYDLNVATRKTLRLKGLL
CEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA