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The map label for this gene is trpE

Identifier: 218694835

GI number: 218694835

Start: 1465148

End: 1466710

Strand: Reverse

Name: trpE

Synonym: EC55989_1422

Alternate gene names: 218694835

Gene position: 1466710-1465148 (Counterclockwise)

Preceding gene: 218694836

Following gene: 218694834

Centisome position: 28.45

GC content: 54.32

Gene sequence:

>1563_bases
ATGCAAACACAAAAACCGACTCTCGAACTGCTAACCTGCGAAGGCGCTTATCGCGACAATCCCACCGCGCTTTTTCACCA
GTTGTGTGGGGATCGTCCGGCAACGCTGCTGCTGGAATCCGCAGATATCGACAGCAAAGATGATTTAAAAAGCCTGCTAC
TGGTAGACAGTGCGCTGCGCATTACAGCATTAGGTGACACTGTCACAATTCAGGCACTTTCCGGCAACGGCGAAGCCCTG
CTGACACTACTGGATAACGCCCTGCCTGCGGGTGTGGAAAATGAACAATCACCAAACTGCCGTGTGCTGCGCTTCCCCCC
TGTCAGTCCACTGCTGGATGAAGACGCTCGCTTATGCTCCCTTTCGGTTTTTGACGCTTTCCGTTTATTGCAGAATCTGT
TGAATGTACCGAAGGAAGAACGAGAAGCAATGTTCTTCGGCGGCCTGTTCTCTTATGACCTTGTGGCGGGATTTGAAGAT
TTACCGCAACTGTCAGCGGAAAATAACTGCCCTGATTTCTGTTTTTATCTCGCTGAAACTCTGATGGTGATTGACCATCA
GAAAAAAAGCACCCGTATTCAGGCCAGCCTGTTTGCTCCGAATGAAGAAGAAAAACAACGTCTCACTGCTCGCCTGAACG
AACTACGTCAGCAACTGACCGAAGCCGCGCCGCCGCTGCCAGTGGTTTCCGTGCCGCATATGCGTTGTGAATGTAATCAG
AGCGATGAAGAGTTCGGTGGCGTAGTGCGTTTGTTGCAAAAAGCGATTCGCGCTGGAGAAATTTTCCAGGTGGTGCCATC
TCGCCGTTTCTCTCTGCCCTGCCCGTCACCGCTGGCGGCCTATTACGTGCTGAAAAAGAGTAATCCCAGCCCGTACATGT
TTTTTATGCAGGATAATGATTTCACCCTGTTTGGCGCGTCGCCGGAAAGTTCGCTCAAGTATGACGCCACCAGCCGCCAG
ATTGAGATCTACCCGATTGCCGGAACACGTCCACGCGGTCGTCGTGCCGATGGCTCGCTGGACAGAGATCTCGACAGCCG
TATTGAACTGGAAATGCGTACCGATCATAAAGAGCTTTCTGAACATCTGATGCTGGTGGATCTCGCCCGTAATGATCTGG
CACGCATTTGCACCCCCGGCAGCCGCTACGTCGCCGATCTTACCAAAGTTGACCGTTACTCTTACGTGATGCACCTGGTC
TCCCGCGTGGTCGGTGAGCTGCGCCACGATCTCGACGCCCTGCACGCTTACCGCGCCTGTATGAATATGGGGACGTTAAG
CGGTGCGCCGAAAGTACGCGCCATGCAGTTAATTGCCGAGGCGGAAGGTCGTCGCCGCGGCAGCTACGGCGGCGCGGTAG
GTTATTTCACCGCGCATGGCGATCTCGACACCTGCATTGTGATCCGCTCGGCGCTGGTGGAAAACGGTATCGCCACCGTG
CAAGCCGGTGCTGGCGTAGTCCTTGATTCTATTCCGCAGTCGGAAGCCGACGAAACCCGTAATAAAGCCCGCGCTGTACT
GCGCGCTATTGCCACCGCGCATCATGCACAGGAGACTTTCTGA

Upstream 100 bases:

>100_bases
ACTTCCTGAAACGGGCAGTGTATTCACCATGCGTAAAGCAATCAGATACCCAGCCCGCCTAATGAGCGGGCTTTTTTTTG
AACAAAATTAGAGAATAACA

Downstream 100 bases:

>100_bases
TGGCTGACATACTGCTGCTCGATAATATCGACTCTTTTACATACAACCTGGCAGATCAGTTGCGCAGCAATGGGCATAAC
GTGGTGATTTACCGCAACCA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALRITALGDTVTIQALSGNGEAL
LTLLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQLTEAAPPLPVVSVPHMRCECNQ
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQ
IEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLV
SRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENGIATV
QAGAGVVLDSIPQSEADETRNKARAVLRAIATAHHAQETF

Sequences:

>Translated_520_residues
MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALRITALGDTVTIQALSGNGEAL
LTLLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQLTEAAPPLPVVSVPHMRCECNQ
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQ
IEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLV
SRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENGIATV
QAGAGVVLDSIPQSEADETRNKARAVLRAIATAHHAQETF
>Mature_520_residues
MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALRITALGDTVTIQALSGNGEAL
LTLLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQLTEAAPPLPVVSVPHMRCECNQ
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQ
IEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLV
SRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENGIATV
QAGAGVVLDSIPQSEADETRNKARAVLRAIATAHHAQETF

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=520, Percent_Identity=99.4230769230769, Blast_Score=1061, Evalue=0.0,
Organism=Escherichia coli, GI1788114, Length=422, Percent_Identity=29.6208530805687, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI87082077, Length=299, Percent_Identity=27.7591973244147, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1786809, Length=227, Percent_Identity=29.0748898678414, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320935, Length=399, Percent_Identity=36.8421052631579, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324361, Length=274, Percent_Identity=28.1021897810219, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57566; Mature: 57566

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEE
ITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCS
EEEECCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
LSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEDLPQLSAENNCPDFCFYLAET
HHHHHHHHHHHHHHCCCHHHHCEEEECCHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHH
LMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQLTEAAPPLPVVSVPHMRCECNQ
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEECCCC
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDND
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHEEEEEECCCCCCEEEEEECCC
FTLFGASPESSLKYDATSRQIEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELS
EEEEECCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
EHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLVSRVVGELRHDLDALHAYRAC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENGIATV
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEE
QAGAGVVLDSIPQSEADETRNKARAVLRAIATAHHAQETF
ECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEE
ITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCS
EEEECCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
LSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEDLPQLSAENNCPDFCFYLAET
HHHHHHHHHHHHHHCCCHHHHCEEEECCHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHH
LMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQLTEAAPPLPVVSVPHMRCECNQ
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEECCCC
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDND
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHEEEEEECCCCCCEEEEEECCC
FTLFGASPESSLKYDATSRQIEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELS
EEEEECCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
EHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLVSRVVGELRHDLDALHAYRAC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENGIATV
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEE
QAGAGVVLDSIPQSEADETRNKARAVLRAIATAHHAQETF
ECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7038627; 7021857; 9097039; 9278503; 351195; 4598537 [H]