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The map label for this gene is hlyE [H]

Identifier: 218694696

GI number: 218694696

Start: 1326117

End: 1326371

Strand: Reverse

Name: hlyE [H]

Synonym: EC55989_1277

Alternate gene names: 218694696

Gene position: 1326371-1326117 (Counterclockwise)

Preceding gene: 218694699

Following gene: 218694692

Centisome position: 25.73

GC content: 31.37

Gene sequence:

>255_bases
TTGTTTGATATTTATCATATTAATAGAAATAAAGACATTGACGCATCCCGCCCGGCTAACTATGAATTAGATGAAGTAAA
ATTTATTAATAGTTGTAAAACAGGAGTTTCATTACAATTTATATATTTAAAGAGGCGAATGATTATGACTGAAATCGTTG
CAGATAAAACGGTAGAAGTAGTTAAAAACGCAATCGAAACTGCAGATGGAGCATTAGATCTTTATAATAAATATCTCGAT
CAGGTCATCCCCTAG

Upstream 100 bases:

>100_bases
CTCACGTGGCCTTCTGGCGACGACGCTCATCCAGCAGAAATGAAAAATATCACCCGGCAAAAAAATAGAATAGAAGCATT
CGCCATAATGACATTAAACA

Downstream 100 bases:

>100_bases
CAGACCTTTGATGAAACCATAAAAGAGTTAAGTCGCTTTAAACAGGAGTATTCACAGGCAGCCTCCGTTTTAGTCGGCGA
TATTAAAACCTTACTTATGG

Product: hypothetical protein

Products: NA

Alternate protein names: Cytotoxin ClyA; Silent hemolysin SheA [H]

Number of amino acids: Translated: 84; Mature: 84

Protein sequence:

>84_residues
MFDIYHINRNKDIDASRPANYELDEVKFINSCKTGVSLQFIYLKRRMIMTEIVADKTVEVVKNAIETADGALDLYNKYLD
QVIP

Sequences:

>Translated_84_residues
MFDIYHINRNKDIDASRPANYELDEVKFINSCKTGVSLQFIYLKRRMIMTEIVADKTVEVVKNAIETADGALDLYNKYLD
QVIP
>Mature_84_residues
MFDIYHINRNKDIDASRPANYELDEVKFINSCKTGVSLQFIYLKRRMIMTEIVADKTVEVVKNAIETADGALDLYNKYLD
QVIP

Specific function: Toxin, which has some hemolytic activity towards mammalian cells. Acts by forming a pore-like structure upon contact with mammalian cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Periplasm (By similarity). Host cell membrane; Single-pass membrane protein (Potential). Note=Exported from the cell by outer membrane vesicles. Also found in the periplasmic space (By similarity) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemolysin E family [H]

Homologues:

Organism=Escherichia coli, GI87081853, Length=36, Percent_Identity=100, Blast_Score=75, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010356 [H]

Pfam domain/function: PF06109 HlyE [H]

EC number: NA

Molecular weight: Translated: 9730; Mature: 9730

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDIYHINRNKDIDASRPANYELDEVKFINSCKTGVSLQFIYLKRRMIMTEIVADKTVEV
CCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VKNAIETADGALDLYNKYLDQVIP
HHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDIYHINRNKDIDASRPANYELDEVKFINSCKTGVSLQFIYLKRRMIMTEIVADKTVEV
CCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VKNAIETADGALDLYNKYLDQVIP
HHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10865950; 11206551; 11258796 [H]