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The map label for this gene is mhpE

Identifier: 218693816

GI number: 218693816

Start: 400047

End: 401060

Strand: Direct

Name: mhpE

Synonym: EC55989_0359

Alternate gene names: 218693816

Gene position: 400047-401060 (Clockwise)

Preceding gene: 218693815

Following gene: 218693817

Centisome position: 7.76

GC content: 56.41

Gene sequence:

>1014_bases
ATGAACGGTAAAAAACTTTATATCTCGGACGTCACGTTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGA
AAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGTGGATTCGATTGAAGTGGCCCATGGCGACGGTTTGCAGG
GTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCAGCGGCGGATGTGGTGAAGCACGCC
AAAATCGCGACGTTGTTGCTGCCAGGAATCGGCACTATTCACGATCTGAAAAATGCCTGGCAGGCTGGCGCGCGGGTGGT
TCGTGTGGCAACGCACTGTACCGAAGCTGATGTTTCCGCCCAGCATATTCAGTATGCCCGCGAGCTCGGAATGGACACCG
TTGGTTTTCTGATGATGAGCCATATGACCACGCCGGAGAATCTCGCAAAGCAGGCAAAGCTGATGGAAGGCTACGGTGCG
ACCTGTATTTATGTGGTGGATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGA
GCTGAAACCAGAAACGCAAACTGGCATGCACGCTCACCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGG
AAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATT
GCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCC
GTTGCAGGATCGACCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCTGGTGTTTACTCGAGCTTCCTGCGTC
ACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGC
GGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAA

Upstream 100 bases:

>100_bases
TGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCG
CGCAAGGCAGGAGAAGCGGC

Downstream 100 bases:

>100_bases
TGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCT
ACATTTCGCCCGTAGGCAGT

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSIEVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHA
KIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVG
GQEDMIVDVALDLRNNK

Sequences:

>Translated_337_residues
MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSIEVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHA
KIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVG
GQEDMIVDVALDLRNNK
>Mature_337_residues
MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSIEVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHA
KIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVG
GQEDMIVDVALDLRNNK

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=337, Percent_Identity=100, Blast_Score=690, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_ECO24 (A7ZI99)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461528.1
- ProteinModelPortal:   A7ZI99
- SMR:   A7ZI99
- STRING:   A7ZI99
- PRIDE:   A7ZI99
- EnsemblBacteria:   EBESCT00000022421
- GeneID:   5587752
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0376
- eggNOG:   COG0119
- GeneTree:   EBGT00050000011169
- HOGENOM:   HBG300122
- OMA:   ATCVYVV
- ProtClustDB:   PRK08195
- BioCyc:   ECOL331111:ECE24377A_0376-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36471; Mature: 36471

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 168-168 BINDING 197-197 BINDING 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSIEVAHGDGLQGSSFNYG
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
FGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGATCIYVVDSGGAMNMSDIRDR
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
FRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAA
EEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
RYGLSAVDILVELGKRRMVGGQEDMIVDVALDLRNNK
HHCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSIEVAHGDGLQGSSFNYG
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
FGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGATCIYVVDSGGAMNMSDIRDR
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
FRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAA
EEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
RYGLSAVDILVELGKRRMVGGQEDMIVDVALDLRNNK
HHCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA