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The map label for this gene is prpE

Identifier: 218693798

GI number: 218693798

Start: 377806

End: 379692

Strand: Direct

Name: prpE

Synonym: EC55989_0341

Alternate gene names: 218693798

Gene position: 377806-379692 (Clockwise)

Preceding gene: 218693797

Following gene: 218693799

Centisome position: 7.33

GC content: 58.03

Gene sequence:

>1887_bases
ATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTG
GCAGACGCCCTTTACGCAAACGCTCGACCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAATTTGT
GCCACAACGCTATCGACCGCTGGCTGGAGAAACAGCCAGAGGCACTGGCATTGATTGCCGTCTCTTCGGAAACAGAAGAA
GAGCGCACCTTTACCTTTCGCCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGCTCACTGGGCGTGCAGCG
CGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGTGCTA
TTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCGTGGCGGCGCGAATTGATGACGCTAAACCGGTGCTGATTGTC
TCGGCTGATGCCGGAGCGCGCGGTGGCAAAATCATTCCCTATAAAAAATTGCTCGACGATGCGATAAGTCAGGCGCAGCA
CCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGT
CGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACT
TCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACAC
CATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTT
ACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAA
ATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGA
AATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGG
TGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGC
GGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGCGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGT
CACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGCTGTATTCAGACCA
TCTGGGGCGACGACGACCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGC
ATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGTCTGGGCAC
GCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAG
GGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGATAGTCTGGAAGATCGTGATGTGGCGCATTCGCAAGAGAAG
GCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGTCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCGAA
AACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCAATTTGCGAAGGACGCGATCCTGGGGATCTGACGACCATTG
ATGATCCTGCGTCATTGGATCAGATCCGCCAGGCGATGGAAGAGTAA

Upstream 100 bases:

>100_bases
CGACAGAACTCGCCTGGAACAGATGCCGGTCAATGAGTATCTCGACCTGTACGTCATTTAAGTAAACGGCGGTAAGGCGT
AAGTTCAACAGGAGAGCATT

Downstream 100 bases:

>100_bases
GGTATTGTCGGATGCGTCGCGTGCCGCATCCGACATCATGCGCCGATGCCTGATGCGACGCTTACCGCGTCTTATTATGC
CTACAGGCCTGAACCGTAGG

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEE
ERTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWK
IVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIAR
GLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWG
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEK
AIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE

Sequences:

>Translated_628_residues
MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEE
ERTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWK
IVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIAR
GLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWG
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEK
AIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE
>Mature_627_residues
SFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEE
RTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVS
ADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYTS
GTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKI
VEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARG
LDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWGI
RDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKA
IMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=636, Percent_Identity=39.937106918239, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI8923896, Length=657, Percent_Identity=35.3120243531202, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI28416953, Length=640, Percent_Identity=36.71875, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI253970489, Length=670, Percent_Identity=35.0746268656716, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI115511026, Length=561, Percent_Identity=27.4509803921569, Blast_Score=159, Evalue=1e-38,
Organism=Homo sapiens, GI122937307, Length=606, Percent_Identity=26.7326732673267, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI42544132, Length=557, Percent_Identity=26.2118491921005, Blast_Score=156, Evalue=7e-38,
Organism=Homo sapiens, GI58082049, Length=569, Percent_Identity=26.713532513181, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI157311624, Length=568, Percent_Identity=26.9366197183099, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI157311622, Length=568, Percent_Identity=26.9366197183099, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI31982927, Length=641, Percent_Identity=23.5569422776911, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI38505220, Length=558, Percent_Identity=25.6272401433692, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI156151445, Length=572, Percent_Identity=22.2027972027972, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI187761345, Length=411, Percent_Identity=24.5742092457421, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI187761343, Length=411, Percent_Identity=24.5742092457421, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1786529, Length=628, Percent_Identity=99.6815286624204, Blast_Score=1291, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=637, Percent_Identity=36.734693877551, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI221142682, Length=544, Percent_Identity=24.6323529411765, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788107, Length=577, Percent_Identity=24.0901213171577, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=23.1499051233397, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI145693145, Length=549, Percent_Identity=21.4936247723133, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=654, Percent_Identity=33.6391437308869, Blast_Score=354, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI71982997, Length=654, Percent_Identity=33.6391437308869, Blast_Score=354, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI17569507, Length=650, Percent_Identity=21.6923076923077, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=549, Percent_Identity=25.3187613843352, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=568, Percent_Identity=22.7112676056338, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=582, Percent_Identity=22.3367697594502, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=536, Percent_Identity=22.2014925373134, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=552, Percent_Identity=20.2898550724638, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319264, Length=633, Percent_Identity=38.5466034755134, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323182, Length=641, Percent_Identity=37.597503900156, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319699, Length=407, Percent_Identity=22.3587223587224, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355225, Length=641, Percent_Identity=38.6895475819033, Blast_Score=442, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24667955, Length=650, Percent_Identity=34.7692307692308, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI62472339, Length=562, Percent_Identity=36.1209964412811, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24667959, Length=529, Percent_Identity=35.7277882797732, Blast_Score=332, Evalue=5e-91,
Organism=Drosophila melanogaster, GI21355181, Length=529, Percent_Identity=23.8185255198488, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21358303, Length=511, Percent_Identity=23.8747553816047, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922652, Length=545, Percent_Identity=23.8532110091743, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648260, Length=380, Percent_Identity=26.5789473684211, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356947, Length=368, Percent_Identity=25.2717391304348, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281365686, Length=519, Percent_Identity=22.7360308285164, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356441, Length=514, Percent_Identity=23.3463035019455, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130357, Length=389, Percent_Identity=25.1928020565553, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648257, Length=377, Percent_Identity=25.9946949602122, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648253, Length=554, Percent_Identity=23.2851985559567, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648255, Length=554, Percent_Identity=23.4657039711191, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 69437; Mature: 69305

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDR
CCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHEEECCCCHHHHHHHHH
WLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAE
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
AHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVSADAGARGGKIIPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHH
AISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
HHHHHHCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCEEEEE
LAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARGLDDRPTRLGSPGVPMYGYN
EECCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCC
VQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWG
HHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECC
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAF
CCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKESDSLEDRDVAHSQEKAIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLDQIRQAMEE
HHCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDR
CHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHEEECCCCHHHHHHHHH
WLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAE
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
AHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVSADAGARGGKIIPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHH
AISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
HHHHHHCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCEEEEE
LAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARGLDDRPTRLGSPGVPMYGYN
EECCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCC
VQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWG
HHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECC
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAF
CCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKESDSLEDRDVAHSQEKAIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLDQIRQAMEE
HHCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]