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The map label for this gene is lpd

Identifier: 218693582

GI number: 218693582

Start: 128216

End: 129640

Strand: Direct

Name: lpd

Synonym: EC55989_0109

Alternate gene names: 218693582

Gene position: 128216-129640 (Clockwise)

Preceding gene: 218693581

Following gene: 218693584

Centisome position: 2.49

GC content: 52.91

Gene sequence:

>1425_bases
ATGAGTACTGAAATCAAAACTCAGGTCGTGGTACTTGGGGCAGGCCCCGCAGGTTACTCCGCTGCCTTCCGTTGCGCTGA
TTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTA
AAGCACTGCTGCACGTAGCAAAAGTTATCGAAGAAGCCAAAGCGCTGGCTGAACACGGTATCGTCTTCGGCGAACCGAAA
ACCGATATTGACAAGATTCGTACCTGGAAAGAGAAAGTGATCAATCAGCTGACCGGTGGTCTGGCTGGTATGGCGAAAGG
CCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTTACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGTAAAA
CCGTGATCAACTTCGACAACGCGATCATTGCAGCGGGTTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCG
CGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGG
TCTGGAAATGGGCACCGTATACCACGCGCTGGGTTCACAGATTGACGTGGTTGAAATGTTCGACCAGGTTATCCCGGCAG
CTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCC
GTTGAAGCGAAAGAAGACGGTATTTATGTGACGATGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGT
GCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCTGGCGTGGAAGTGGACGACCGTGGTT
TCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAGCCGATGCTG
GCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACACTACTTCGATCCGAAAGTTAT
CCCGTCCATCGCCTATACCGAACCAGAAGTTGCATGGGTAGGTCTGACTGAGAAAGAAGCGAAAGAGAAAGGCATCAGCT
ATGAAACCGCCACCTTCCCGTGGGCTGCTTCTGGTCGTGCTATCGCTTCCGACTGCGCAGACGGTATGACCAAGCTGATT
TTCGACAAAGAATCTCACCGTGTGATCGGTGGTGCGATTGTCGGTACCAACGGCGGCGAGCTGCTGGGTGAAATCGGCCT
GGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGCC
TGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
TTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGAAAGACGACGGGTATGACCGCCGGAG
ATAAATATATAGAGGTCATG

Downstream 100 bases:

>100_bases
TTGTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCC
TGTCGCCTTGCTCCGCGCTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPK
TDIDKIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLI
FDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK

Sequences:

>Translated_474_residues
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPK
TDIDKIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLI
FDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK
>Mature_473_residues
STEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKT
DIDKIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDPR
IWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTAV
EAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLA
HKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIF
DKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=43.6123348017621, Blast_Score=345, Evalue=6e-95,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=28.4463894967177, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI22035672, Length=429, Percent_Identity=29.3706293706294, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI148277071, Length=437, Percent_Identity=27.6887871853547, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI148277065, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI33519430, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI33519428, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI33519426, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=26.7573696145125, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI291045268, Length=432, Percent_Identity=25.2314814814815, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=100, Blast_Score=957, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=27.4089935760171, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=28.3516483516484, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=27.9176201372998, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=448, Percent_Identity=39.5089285714286, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=27.7310924369748, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=27.0642201834862, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=27.0642201834862, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=442, Percent_Identity=26.6968325791855, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=40.7894736842105, Blast_Score=308, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=27.0788912579957, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=27.2151898734177, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=39.6061269146608, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24640549, Length=462, Percent_Identity=29.004329004329, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24640553, Length=462, Percent_Identity=29.004329004329, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640551, Length=462, Percent_Identity=29.004329004329, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=26.3048016701461, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH_ECO57 (P0A9P2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   H85494
- PIR:   H90643
- RefSeq:   NP_285812.1
- RefSeq:   NP_308147.1
- ProteinModelPortal:   P0A9P2
- SMR:   P0A9P2
- EnsemblBacteria:   EBESCT00000024684
- EnsemblBacteria:   EBESCT00000059894
- GeneID:   913660
- GeneID:   956813
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0126
- KEGG:   ecs:ECs0120
- GeneTree:   EBGT00050000009315
- HOGENOM:   HBG515043
- OMA:   MMDGLMA
- ProtClustDB:   PRK06467
- BioCyc:   ECOL83334:ECS0120-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 50689; Mature: 50558

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 445-445 BINDING 54-54 BINDING 117-117 BINDING 205-205 BINDING 238-238 BINDING 313-313 BINDING 321-321

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEEECCCCCCHHHHHHHHCCHHEEEEHHHHCCCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCHHCC
GANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLV
CCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
MGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTA
HCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQ
EEECCCCEEEEECCCCCCCCCHHHCEEEEEEECCCCCCCCCCCCCCCEECCCCEEEECHH
LRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCEECCCCCEEEE
GLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIFDKESHRVIGGAIVGTNGGE
ECCHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVA
CCCEEEEEEEEECCCCCCHHHHHHHHCCHHEEEEHHHHCCCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCHHCC
GANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLV
CCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
MGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTA
HCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQ
EEECCCCEEEEECCCCCCCCCHHHCEEEEEEECCCCCCCCCCCCCCCEECCCCEEEECHH
LRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCEECCCCCEEEE
GLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIFDKESHRVIGGAIVGTNGGE
ECCHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796