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The map label for this gene is btuB [H]

Identifier: 229598867

GI number: 229598867

Start: 4624246

End: 4626084

Strand: Direct

Name: btuB [H]

Synonym: ECED1_4675

Alternate gene names: 229598867

Gene position: 4624246-4626084 (Clockwise)

Preceding gene: 218692254

Following gene: 218692257

Centisome position: 88.76

GC content: 47.8

Gene sequence:

>1839_bases
ATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATAC
TCTCGTCGTTACTGCTAACCGTTTTCAACAGCCTATCAATACTGTTCTGGCTCCGACGGATATTGTCACGCGTGAGGATA
TCGAACGCTGGCAATCCAGAACCGTTCTGGATGTGATGCGCCGCTTACCTGGTGTTGATGTCGCACAGTATGGCGGCATG
GGACAGAGTTCTTCTCTTTTTATCCGGGGAACCGAATCGAAACACGTTCTGGTGTTAGTTGATGGAATTCCAGTAGCACG
CCCTGGTATATCGAATAGCTCAGACATTGACCAGATCCCAGTCTCGCTGGTCCAGCGTATTGAATATATTCGTGGCCCAC
GCTCGGCTGTTTATGGCTCTGGTGCAATTGGTGGTGTTGTCAACATCATCACTATGACGAATAAAGAGCGTTCGCAAATT
AACTCCAGTATCGGTTCGAAAGGCTACCAACAGTACGATGGCACATACCGCCAGCGTTTTGGAGATACGGTGGTGACTGC
TGCAGGGGCTTATCAGACGACCAAAGGTTTCAATGTTCAGCCACACTCAAGTTATAGCGGAGATGGCGATCGCGATGGTT
ATCGTAACAAGCTGTTCTGGGGGGGAATTGAGCACAAATTTAACGATCAATTTGATGGTTACTTCCGTGGCTACGGTTAT
GCCAGTAATGCCGATTACGACCAAGGGAACTGGGGATATGATGGCGGCAATGATGAGCATCAAAATTACACACAGTCATG
GGATACTGGATTGCGTTTTAATTCGGGAGTCTACTCTTCGCAACTGGTGGTAAATTATCAGCGCATTAAGGACTACAACT
ACAGCAGTCTGACTGGGCGTTACGCATCAGGTTATACACTTGATGATATGGAGCAACGTTACATCCAGTGGGGTAACAAT
GTCATTGTTGGGCATGGTGCTATCAGCGCAGGAGCAGACTGGAAACAGGAAAAACTGATGTCTTTTGGCGCTAATGGCAC
GGATAACTACAAGCGTAATAACACTGGGCTTTATCTAACCGGGCAGCAGCAAATTGATAACATCACACTAGAAGCCTCTG
GTCGTGAGGATCACGATGGCCAGCTAGGTTGGCATGGCACTTGGCAAACGGCAGCAGGGTGGCAGTTTGTTGATGGTTAC
AAAGTAACGCTTTCCTATGGAACTGGTTTCCTTGCTCCTTCGCTTGGTCAACAATTTGGCGCAGAGCGGTTTGGTATTGC
TTCTAACCCTAACTTGAAACCAGAAGAATCTAAGCAATGGGAGGCAGGATTAGAAGGCTTGACAGGTCCTGTTGACTGGC
GATTGTCGACCTATCGCTACAAAATTCAGAACCTGATCGACTACAACAACAATACCTATTTTAACGTTAAATCCGCGACC
ATTAAGGGCCTGGAATGGACGGGTAATTTCACAACTGGTCCTATCGATCATCGTTTAACGTTGCAGTATGTAGACCCCCG
TGATGATGAAACTGATCAACAACTCTATCGCCGTGCAAAACAGCAGGTAAAATATGAGTTGAGCGGTGAGGTTTACGAGT
TGGGGTGGGATGTCACGTATCAGTACATTGGTGAACGATACGATTACGACTATGACAGTACCCGTAAGGTGAAAATGGGG
GGACTGAGCGTGTGGGATATCGGACTTTCATATCCGGTCACCTCTCACCTGACAGTTCGTGGTAAAATAGCCAACCTGTT
CGACAAAGATTACGAGACAGTCTATGGCTACCAAACTGCAGGACGGGAATATACCTTGTCTGGCAGCTACACCTTCTGA

Upstream 100 bases:

>100_bases
CAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCT
TCTATTGTGGATGCTTTACA

Downstream 100 bases:

>100_bases
ACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTGCCGG
ATCTCCATTACATTTATGCT

Product: vitamin B12/cobalamin outer membrane transporter

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPINTVLAPTDIVTREDIERWQSRTVLDVMRRLPGVDVAQYGGM
GQSSSLFIRGTESKHVLVLVDGIPVARPGISNSSDIDQIPVSLVQRIEYIRGPRSAVYGSGAIGGVVNIITMTNKERSQI
NSSIGSKGYQQYDGTYRQRFGDTVVTAAGAYQTTKGFNVQPHSSYSGDGDRDGYRNKLFWGGIEHKFNDQFDGYFRGYGY
ASNADYDQGNWGYDGGNDEHQNYTQSWDTGLRFNSGVYSSQLVVNYQRIKDYNYSSLTGRYASGYTLDDMEQRYIQWGNN
VIVGHGAISAGADWKQEKLMSFGANGTDNYKRNNTGLYLTGQQQIDNITLEASGREDHDGQLGWHGTWQTAAGWQFVDGY
KVTLSYGTGFLAPSLGQQFGAERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSTYRYKIQNLIDYNNNTYFNVKSAT
IKGLEWTGNFTTGPIDHRLTLQYVDPRDDETDQQLYRRAKQQVKYELSGEVYELGWDVTYQYIGERYDYDYDSTRKVKMG
GLSVWDIGLSYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF

Sequences:

>Translated_612_residues
MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPINTVLAPTDIVTREDIERWQSRTVLDVMRRLPGVDVAQYGGM
GQSSSLFIRGTESKHVLVLVDGIPVARPGISNSSDIDQIPVSLVQRIEYIRGPRSAVYGSGAIGGVVNIITMTNKERSQI
NSSIGSKGYQQYDGTYRQRFGDTVVTAAGAYQTTKGFNVQPHSSYSGDGDRDGYRNKLFWGGIEHKFNDQFDGYFRGYGY
ASNADYDQGNWGYDGGNDEHQNYTQSWDTGLRFNSGVYSSQLVVNYQRIKDYNYSSLTGRYASGYTLDDMEQRYIQWGNN
VIVGHGAISAGADWKQEKLMSFGANGTDNYKRNNTGLYLTGQQQIDNITLEASGREDHDGQLGWHGTWQTAAGWQFVDGY
KVTLSYGTGFLAPSLGQQFGAERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSTYRYKIQNLIDYNNNTYFNVKSAT
IKGLEWTGNFTTGPIDHRLTLQYVDPRDDETDQQLYRRAKQQVKYELSGEVYELGWDVTYQYIGERYDYDYDSTRKVKMG
GLSVWDIGLSYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF
>Mature_612_residues
MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPINTVLAPTDIVTREDIERWQSRTVLDVMRRLPGVDVAQYGGM
GQSSSLFIRGTESKHVLVLVDGIPVARPGISNSSDIDQIPVSLVQRIEYIRGPRSAVYGSGAIGGVVNIITMTNKERSQI
NSSIGSKGYQQYDGTYRQRFGDTVVTAAGAYQTTKGFNVQPHSSYSGDGDRDGYRNKLFWGGIEHKFNDQFDGYFRGYGY
ASNADYDQGNWGYDGGNDEHQNYTQSWDTGLRFNSGVYSSQLVVNYQRIKDYNYSSLTGRYASGYTLDDMEQRYIQWGNN
VIVGHGAISAGADWKQEKLMSFGANGTDNYKRNNTGLYLTGQQQIDNITLEASGREDHDGQLGWHGTWQTAAGWQFVDGY
KVTLSYGTGFLAPSLGQQFGAERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSTYRYKIQNLIDYNNNTYFNVKSAT
IKGLEWTGNFTTGPIDHRLTLQYVDPRDDETDQQLYRRAKQQVKYELSGEVYELGWDVTYQYIGERYDYDYDSTRKVKMG
GLSVWDIGLSYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=622, Percent_Identity=56.7524115755627, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1788478, Length=687, Percent_Identity=24.8908296943231, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786798, Length=712, Percent_Identity=24.1573033707865, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68741; Mature: 68741

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00430 TONB_DEPENDENT_REC_1 ; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPINTVLAPTDIVTREDIERWQSR
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHCC
TVLDVMRRLPGVDVAQYGGMGQSSSLFIRGTESKHVLVLVDGIPVARPGISNSSDIDQIP
CHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHH
VSLVQRIEYIRGPRSAVYGSGAIGGVVNIITMTNKERSQINSSIGSKGYQQYDGTYRQRF
HHHHHHHHHHCCCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHCCHHHHHC
GDTVVTAAGAYQTTKGFNVQPHSSYSGDGDRDGYRNKLFWGGIEHKFNDQFDGYFRGYGY
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEECCCC
ASNADYDQGNWGYDGGNDEHQNYTQSWDTGLRFNSGVYSSQLVVNYQRIKDYNYSSLTGR
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCCCCCEEEEEEHHHCCCCCCCCCCC
YASGYTLDDMEQRYIQWGNNVIVGHGAISAGADWKQEKLMSFGANGTDNYKRNNTGLYLT
CCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEE
GQQQIDNITLEASGREDHDGQLGWHGTWQTAAGWQFVDGYKVTLSYGTGFLAPSLGQQFG
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCHHHHHHC
AERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSTYRYKIQNLIDYNNNTYFNVKSAT
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHEEEHHHHCCCCCEEEEEECCE
IKGLEWTGNFTTGPIDHRLTLQYVDPRDDETDQQLYRRAKQQVKYELSGEVYELGWDVTY
EEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEEEECCCEEEECCCEEE
QYIGERYDYDYDSTRKVKMGGLSVWDIGLSYPVTSHLTVRGKIANLFDKDYETVYGYQTA
HHHHHHCCCCCCCCCEEEECCEEEEECCCCCCCCCEEEEEEHHHHHHCCCCHHEEEEECC
GREYTLSGSYTF
CCEEEECCCCCC
>Mature Secondary Structure
MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPINTVLAPTDIVTREDIERWQSR
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHCC
TVLDVMRRLPGVDVAQYGGMGQSSSLFIRGTESKHVLVLVDGIPVARPGISNSSDIDQIP
CHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHH
VSLVQRIEYIRGPRSAVYGSGAIGGVVNIITMTNKERSQINSSIGSKGYQQYDGTYRQRF
HHHHHHHHHHCCCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHCCHHHHHC
GDTVVTAAGAYQTTKGFNVQPHSSYSGDGDRDGYRNKLFWGGIEHKFNDQFDGYFRGYGY
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEECCCC
ASNADYDQGNWGYDGGNDEHQNYTQSWDTGLRFNSGVYSSQLVVNYQRIKDYNYSSLTGR
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCCCCCEEEEEEHHHCCCCCCCCCCC
YASGYTLDDMEQRYIQWGNNVIVGHGAISAGADWKQEKLMSFGANGTDNYKRNNTGLYLT
CCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEE
GQQQIDNITLEASGREDHDGQLGWHGTWQTAAGWQFVDGYKVTLSYGTGFLAPSLGQQFG
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCHHHHHHC
AERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSTYRYKIQNLIDYNNNTYFNVKSAT
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHEEEHHHHCCCCCEEEEEECCE
IKGLEWTGNFTTGPIDHRLTLQYVDPRDDETDQQLYRRAKQQVKYELSGEVYELGWDVTY
EEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEEEECCCEEEECCCEEE
QYIGERYDYDYDSTRKVKMGGLSVWDIGLSYPVTSHLTVRGKIANLFDKDYETVYGYQTA
HHHHHHCCCCCCCCCEEEECCEEEEECCCCCCCCCEEEEEEHHHHHHCCCCHHEEEEECC
GREYTLSGSYTF
CCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA