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The map label for this gene is sucB [H]

Identifier: 218692338

GI number: 218692338

Start: 4729840

End: 4730994

Strand: Direct

Name: sucB [H]

Synonym: ECED1_4771

Alternate gene names: 218692338

Gene position: 4729840-4730994 (Clockwise)

Preceding gene: 218692337

Following gene: 218692339

Centisome position: 90.79

GC content: 53.85

Gene sequence:

>1155_bases
ATGATCGAAATTACTGTCCCTGTATTACCGGAATCTGTCACGGAAGGCACGCTGACAACCTGGTGTAAACAAGAAGGCGA
GCACGTAAAACGCGATGATGTGATTGCCGAGCTGGAAACCGATAAAGTCATTCTGGAAATACCGGCCCCGCACGATGGCG
TGTTAAGCAATATTATCGTCAGCGAAGGTAGCACGGTCACGTCCGCGCAACTGTTGGCGCATCTTAAGCCGCAAGCTGTC
ATAGAAGAAACCGTCACACCAGTCACCGAAACCCTGGCGATGCCTTCCGCACGGCTGGAGGCACAGCGTAGTGGTGTCGA
ACTTGCTGACGTTGCGGGGAGCGGGAGAAATGGACGTATCCTGAAAGAGGATGTACAGCGCGTTACTCCTGCACCAGTCA
TTCAGCCTGAACGGGTGGCGGAGATCGCGCCAGCTAAACCCTTGACACCGGGTGCTCGTCAGGAACGTCGTGAGCCGATG
TCGCGCTTACGTCAGCGGATTGCTGAGCGTCTGCTGGCTTCCCAACAAAATAACGCCATTCTTACCACATTCAACGAAGT
AAACATGCAGAGCGTGATGGATTTACGCGCCCGCTGGAAAGATCGTTTTGCCGAGAAGCACGGCGTGAAGCTGGGCTTTA
TGTCCTTCTTTGTTAAGGCCGTTACCCGGGCGCTGGAGCGCTTCCCTGTAGTGAATGCCAGCGTTGATGGCAACGAGATT
ATCTGGCGTGATTATTGCGATATCGGCATTGCAGTGAGCAGCAACCGCGGCCTGGTAGTGCCTGTGTTACGCAATGCGCA
ATCACTCTCTTTGGTGGAAATTGAACGGCAAATTGCCGAATACGCCACACAGGCACGCAATGGCAAATTGCCGCTGGAGG
CGTTACAGGGCGGCACGTTCTCTATCACCAACGGCGGTACTTTTGGTTCGATGATGTCAACGCCAATCATTAACCCGCCG
CAGTCGGCCATTCTTGGGATGCATGCCATCACGCCGCGCCCGGTCGCAGAAAACGGCCAGGTTGTTATTCGTCCCATGAT
GTACCTTGCTCTCAGCTATGACCATCGCATTATCGATGGTCAGGAAGCCGTGCAGACATTGGTGGCAATACGTGAGCTAC
TGGAATCACCGGAACAACTGTTGCTGGATCTTTAA

Upstream 100 bases:

>100_bases
ATGGGCGTGTACATAAACAACAAATCGACGAATTTCTTGCTGCTGCGTTTGCAGATATTCAGCCATGAAAGGCGCACAAA
AATAATAAGGAAAACATTTT

Downstream 100 bases:

>100_bases
GGAGGGCGCCGATGAGTACAATTTTTGATGTGGCAGTTATGGGGGGGGGCCAGGTGGTTACGTAGCGGCACTGCGGGCTG
CGCAGAATGGGCTTTCCGTA

Product: dihydrolipoamide succinyltransferase (E2 component)

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAV
IEETVTPVTETLAMPSARLEAQRSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPM
SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFVKAVTRALERFPVVNASVDGNEI
IWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPP
QSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL

Sequences:

>Translated_384_residues
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAV
IEETVTPVTETLAMPSARLEAQRSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPM
SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFVKAVTRALERFPVVNASVDGNEI
IWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPP
QSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL
>Mature_384_residues
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAV
IEETVTPVTETLAMPSARLEAQRSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPM
SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFVKAVTRALERFPVVNASVDGNEI
IWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPP
QSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=391, Percent_Identity=43.7340153452685, Blast_Score=308, Evalue=4e-84,
Organism=Homo sapiens, GI110671329, Length=421, Percent_Identity=28.2660332541568, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=28.7703016241299, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI203098753, Length=457, Percent_Identity=26.4770240700219, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI203098816, Length=457, Percent_Identity=26.2582056892779, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.1614906832298, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786946, Length=402, Percent_Identity=50.2487562189055, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786305, Length=406, Percent_Identity=29.064039408867, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=403, Percent_Identity=41.1910669975186, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=443, Percent_Identity=27.765237020316, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=25.2358490566038, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=30.4918032786885, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=390, Percent_Identity=43.0769230769231, Blast_Score=322, Evalue=8e-89,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=29.3986636971047, Blast_Score=152, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24645909, Length=234, Percent_Identity=53.8461538461538, Blast_Score=267, Evalue=8e-72,
Organism=Drosophila melanogaster, GI18859875, Length=417, Percent_Identity=26.8585131894484, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=32.0175438596491, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=32.0175438596491, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42179; Mature: 42179

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIV
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHEEECCCEEEEECCCCCCHHHHHHHH
SEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQRSGVELADVAGSGRNGRI
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEECCCCCCCCCC
LKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPMSRLRQRIAERLLASQQNNAI
HHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFVKAVTRALERFPVVNASVDGNEI
EEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEE
IWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATQARNGKLPLEALQGGTF
EEEECCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEE
SITNGGTFGSMMSTPIINPPQSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDG
EEECCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHCCCCEECH
QEAVQTLVAIRELLESPEQLLLDL
HHHHHHHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIV
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHEEECCCEEEEECCCCCCHHHHHHHH
SEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQRSGVELADVAGSGRNGRI
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEECCCCCCCCCC
LKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPMSRLRQRIAERLLASQQNNAI
HHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFVKAVTRALERFPVVNASVDGNEI
EEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEE
IWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATQARNGKLPLEALQGGTF
EEEECCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEE
SITNGGTFGSMMSTPIINPPQSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDG
EEECCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHCCCCEECH
QEAVQTLVAIRELLESPEQLLLDL
HHHHHHHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]