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The map label for this gene is dapF [H]

Identifier: 218692085

GI number: 218692085

Start: 4430665

End: 4431489

Strand: Direct

Name: dapF [H]

Synonym: ECED1_4494

Alternate gene names: 218692085

Gene position: 4430665-4431489 (Clockwise)

Preceding gene: 218692084

Following gene: 218692086

Centisome position: 85.05

GC content: 55.03

Gene sequence:

>825_bases
ATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACC
GGAGCTGATTCGTCGCCTGGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATC
CTGAACTGGATTTTCACTATCGCATTTTCAATACTGATGGCAGTGAAGTGGCGCAGTGCGGCAACGGAGCACGCTGCTTT
GCCCGTTTTGTGCGTCTGAAAGGGCTGACCAATAAGCGCGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTTAC
CGTCACCGATGATGATCTGGTTCGCGTAAACATGGGCGAGCCCAACTTCGAACCTTCCGCTGTGCCGTTTCGCGCTAACA
AAGTGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGTGGCGTGGTGTCGATGGGAAATCCGCACTGC
GTGATTCAGGTCGATGATGTCGATACCGCGGCGGTAGAAACGCTTGGTCCTGTTCTGGAAAGCCACGAGCGTTTTCCGGA
GCGCGCCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAA
CCCAGGCCTGCGGCAGCGGCGCATGTGCGGCGGTGGCGGTAGGGATTCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTG
GAACTCCCCGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGT
CTACGACGGATTTATTCATCTATGA

Upstream 100 bases:

>100_bases
ATCCACGGTGCCGGATAAAAACGACCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTAC
CCGCGTGATTGGAGTAAATG

Downstream 100 bases:

>100_bases
AGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGCGGTTGTCGATTATCTGATTAAAAATCCT
GAGTTTTTTATCCGCAATGC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHYRIFNTDGSEVAQCGNGARCF
ARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKVEKTYIMRAAEQTILCGVVSMGNPHC
VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL

Sequences:

>Translated_274_residues
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHYRIFNTDGSEVAQCGNGARCF
ARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKVEKTYIMRAAEQTILCGVVSMGNPHC
VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL
>Mature_274_residues
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHYRIFNTDGSEVAQCGNGARCF
ARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKVEKTYIMRAAEQTILCGVVSMGNPHC
VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=99.2700729927007, Blast_Score=562, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30267; Mature: 30267

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEE
RIFNTDGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGE
EEEECCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCC
PNFEPSAVPFRANKVEKTYIMRAAEQTILCGVVSMGNPHCVIQVDDVDTAAVETLGPVLE
CCCCCCCCCEECCCHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
SHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
HHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEHEEE
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL
ECCCCEEEEEEECCCCEEEEECCEEEEECCEEEC
>Mature Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEE
RIFNTDGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGE
EEEECCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCC
PNFEPSAVPFRANKVEKTYIMRAAEQTILCGVVSMGNPHCVIQVDDVDTAAVETLGPVLE
CCCCCCCCCEECCCHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
SHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
HHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEHEEE
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL
ECCCCEEEEEEECCCCEEEEECCEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA