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The map label for this gene is ilvG [H]

Identifier: 218692048

GI number: 218692048

Start: 4387362

End: 4389008

Strand: Direct

Name: ilvG [H]

Synonym: ECED1_4454

Alternate gene names: 218692048

Gene position: 4387362-4389008 (Clockwise)

Preceding gene: 218692047

Following gene: 218692049

Centisome position: 84.22

GC content: 54.04

Gene sequence:

>1647_bases
ATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAAT
TATGCCGGTTTACGATGCATTGTATGACGGTGGCGTGGAACACTTGCTGTGCCGACACGAGCAAGGTGCAGCAATGGCGG
CTATCGGTTATGCCCGTGCTACCGGCAAAACTGGCGTTTGCATCGCCACGTCTGGTCCAGGTGCAACCAACCTGATAACC
GGGCTTGCGGACGCGCTGTTAGATTCCATCCCCGTTGTTGCTATCACCGGTCAGGTATCCGCACCGTTTATCGGCACTGA
CGCATTTCAGGAGGTGGATATTCTGGGGTTGTCGTTAGCCTGCACCAAGCACAGTTTCCTGGTGCAATCACTGGAAGAGT
TACCGCGTATCATGGCCGAAGCGTTTGACGTCGCCAGCTCTGGTCGTCCTGGTCCGGTTTTGGTCGATATTCCAAAAGAT
ATCCAGTTAGCCAGCGGCGATCTGGAACCGTGGTTCACCACTGTTGAAAACGAAGTGACTTTCTCACATGCCGAAGTCGA
GCAAGCGCGTCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGCATGGCGCAGGCAGTTT
CGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGTTGAAAGGGCTGGGCGCAGTAGAAGCAGAT
TATCCGTACTATCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCGAACTTCGCAGTGCAGGAGTGCGATCTACTGAT
CGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGCGTTATCCATATGG
ATATCGATCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAGGGTGATTTAAATGCTCTGTTACCAGCA
TTACAGCAGCCGTTAAATATCGATGACTGGCAGCAACACTGCGCGCAACTGCGTGATGAACATGCCTGGCGTTACGACCA
TCCTGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAATCTGCGGATTGCATCGTGACCACAG
ATGTGGGGCAGCACCAGATGTGGGCTGCCCAGCACATCGTCCACACTCGCCCGGAAAACTTCATCACCTCCAGCGGCTTA
GGCACCATGGGTTTCGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACTGTCGTCTGTATCTCCGG
TGACGGCTCTTTCATGATGAATGTGCAAGAACTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCG
ATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATACAGCGAAACTACCCTTACCGAT
AACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCATAAAGACCAGGTTGAAGCGGC
ACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGG
TGCCGCCTGGTGCCAGTAATTCTGAAATGTTGGAGAAATTATCATGA

Upstream 100 bases:

>100_bases
GTCCGGGGGTTTTTTTTGACCTTAAAAACATAACTGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGA
TTCAGGACGGGGAACTAACT

Downstream 100 bases:

>100_bases
TGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCGGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGT
TTCCACGTCTGCTCAATGAA

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIATSGPGATNLIT
GLADALLDSIPVVAITGQVSAPFIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
IQLASGDLEPWFTTVENEVTFSHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALREFLAATKMPATCTLKGLGAVEAD
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPA
LQQPLNIDDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPENFITSSGL
GTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
NPDFLMLASAFGIPGQHITHKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS

Sequences:

>Translated_548_residues
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIATSGPGATNLIT
GLADALLDSIPVVAITGQVSAPFIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
IQLASGDLEPWFTTVENEVTFSHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALREFLAATKMPATCTLKGLGAVEAD
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPA
LQQPLNIDDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPENFITSSGL
GTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
NPDFLMLASAFGIPGQHITHKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS
>Mature_548_residues
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIATSGPGATNLIT
GLADALLDSIPVVAITGQVSAPFIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
IQLASGDLEPWFTTVENEVTFSHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALREFLAATKMPATCTLKGLGAVEAD
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPA
LQQPLNIDDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPENFITSSGL
GTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
NPDFLMLASAFGIPGQHITHKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.3816254416961, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI21361361, Length=499, Percent_Identity=24.8496993987976, Blast_Score=130, Evalue=4e-30,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=46.9202898550725, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=44.2273534635879, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=30.2325581395349, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI1787096, Length=541, Percent_Identity=30.4990757855823, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788716, Length=552, Percent_Identity=27.8985507246377, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=27.1604938271605, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=27.1604938271605, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=571, Percent_Identity=23.6427320490368, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=581, Percent_Identity=43.2013769363167, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=22.9038854805726, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19922626, Length=559, Percent_Identity=25.7602862254025, Blast_Score=171, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59157; Mature: 59157

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEEHHHHHC
TGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDILGLSLA
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCCHHHHHHHEEEEEH
CTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKDIQLASGDLEPWFTTVENEVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEECCCCCCCHHHHHCCCEE
FSHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALREFLAATKMPATCTLKGLGAVEAD
ECHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEM
CCHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
NKLRQAHVALQGDLNALLPALQQPLNIDDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQ
HHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LSDRKSADCIVTTDVGQHQMWAAQHIVHTRPENFITSSGLGTMGFGLPAAVGAQVARPND
HCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHCCEEECCCC
TVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
EEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
NPDFLMLASAFGIPGQHITHKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASN
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
SEMLEKLS
HHHHHHCC
>Mature Secondary Structure
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEEHHHHHC
TGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDILGLSLA
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCCHHHHHHHEEEEEH
CTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKDIQLASGDLEPWFTTVENEVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEECCCCCCCHHHHHCCCEE
FSHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALREFLAATKMPATCTLKGLGAVEAD
ECHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEM
CCHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
NKLRQAHVALQGDLNALLPALQQPLNIDDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQ
HHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LSDRKSADCIVTTDVGQHQMWAAQHIVHTRPENFITSSGLGTMGFGLPAAVGAQVARPND
HCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHCCEEECCCC
TVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
EEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
NPDFLMLASAFGIPGQHITHKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASN
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
SEMLEKLS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]