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The map label for this gene is malS [H]

Identifier: 218691860

GI number: 218691860

Start: 4178632

End: 4180662

Strand: Direct

Name: malS [H]

Synonym: ECED1_4259

Alternate gene names: 218691860

Gene position: 4178632-4180662 (Clockwise)

Preceding gene: 218691858

Following gene: 218691861

Centisome position: 80.21

GC content: 52.44

Gene sequence:

>2031_bases
ATGAAACTCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGATTCCCTGC
CTTTAGCGAACAGGGGACGGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAATGCTAAATTTTG
ACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCT
CAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAACTAGACACCCGCTCCGGTACGCCAACATTGATGATTTCCAT
CCAGAACGCCGTCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACACTGGATGTCA
GCGCCACTTTTGCGGAGGGAGCCGCCGTCCGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAACG
TTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCA
TAACGCCACGGTTTACTTTGTGCTGACAGATCGTTTCGAAAACGGCGATCCCAATAATGACCAGAGTTACGGACGTCATA
AAGACGGTATGGCGGAAATTGGCACTTTTCACGGCGGCGATTTACGCGGCCTGACCAACAAACTGGATTATCTCCAGCAG
TTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGTGATTT
CCCGCATTATGCCTATCATGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAGTAAAGCCGATCTAC
GGACGCTGGTTGATAGCGCGCATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACG
CTGGCGGATATGCAGGAGTATCAGTTTGGCGCGTTATATCTTTCTGGTGACGAGCTGAAAAAAACGCTGGGTGAACGCTG
GAGCGACTGGAAACCTGCTGCCGGGCAAACCTGGCATAGCTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGG
ATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCG
CTGGCCTTTTTGCCGGATATCAAAACCGAATCAACTAGCGCTTCTGGTCTGCCAGTGTTCTATAAAAACAAAACGGATAC
CCACGCTAAAGACATCGACGGCTTTACCCCTCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGA
TTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCTGCA
CTTCGCGAATGGAAAAAAGCCAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCA
CGGCGTGATGCAAAGTGACTACTATTGCTACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAG
CAGTCGATTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGATTTCAACGTGTTGAGCTAC
CTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTATTATTAGCGCCAGGCGCGGT
ACAAATCTTTTATGGCGATGAATCCTCGCGTCCGTTCGGTCCTACCGGTTCTGATCCGCTGCAAGGTACGCGTTCGGATA
TGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTCGCGCACTGGCAAAAAATCAGCCAGTTCCGCGCCCGCCAT
CCCGCAATTGGCGCGGGCAAACAAACGACACTTTCGCTGAAGCAGGGCTACGGCTTTGTTCGTGAGCATGGCGACGATAA
AGTGCTGGTCATCTGGGCTGGGCAACAGTGA

Upstream 100 bases:

>100_bases
CCTTGCTTCTCATCCTGTGGACTTACCGCTCAGGGATGAGTTTTGTCTGGCTTATCGCTGGCAAACTGTCTGAAATCGCA
GCAATAAGGACTCATCCGCC

Downstream 100 bases:

>100_bases
CTTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGAAGCCGCCAACCAGGATAAAATGTCAGCCCTAATCAGCATTGCAGGA
TAAAGCACCGCTCACTCTTC

Product: periplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLMLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPNNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGSKADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTSASGLPVFYKNKTDTHAKDIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYCYGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSY
LSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARH
PAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ

Sequences:

>Translated_676_residues
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLMLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPNNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGSKADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTSASGLPVFYKNKTDTHAKDIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYCYGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSY
LSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARH
PAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ
>Mature_676_residues
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLMLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPNNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGSKADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTSASGLPVFYKNKTDTHAKDIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYCYGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSY
LSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARH
PAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=676, Percent_Identity=97.0414201183432, Blast_Score=1367, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=521, Percent_Identity=24.5681381957774, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 75759; Mature: 75759

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLMLNFDQQCWQP
CCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHCCC
ADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRE
HHHHHHHHHEEECCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHH
CPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQITLQPAATSNGLLLLERAETDT
CCCCCCCEEEEECCCHHHCCCHHHHHHCCEEEEEECCEEEEEECCCCCCEEEEEECCCCC
SAPFDWHNATVYFVLTDRFENGDPNNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGSKADLRTLVDSA
CCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCCCHHHHHHHHHHH
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCC
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTSASGLPVF
HHHEECCCCCCCCHHHHCCCCEECCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCEEE
YKNKTDTHAKDIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
ECCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYCYGFDAMINFDYQEQAAKAVDCLAQ
HHHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHH
MDTTWQQMAEKLQDFNVLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFG
HCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCCHH
REHGDDKVLVIWAGQQ
HCCCCCEEEEEECCCC
>Mature Secondary Structure
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLMLNFDQQCWQP
CCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHCCC
ADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRE
HHHHHHHHHEEECCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHH
CPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQITLQPAATSNGLLLLERAETDT
CCCCCCCEEEEECCCHHHCCCHHHHHHCCEEEEEECCEEEEEECCCCCCEEEEEECCCCC
SAPFDWHNATVYFVLTDRFENGDPNNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGSKADLRTLVDSA
CCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCCCHHHHHHHHHHH
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCC
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTSASGLPVF
HHHEECCCCCCCCHHHHCCCCEECCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCEEE
YKNKTDTHAKDIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
ECCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYCYGFDAMINFDYQEQAAKAVDCLAQ
HHHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHH
MDTTWQQMAEKLQDFNVLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFG
HCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCCHH
REHGDDKVLVIWAGQQ
HCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]