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The map label for this gene is gadX [H]

Identifier: 218691800

GI number: 218691800

Start: 4101471

End: 4102295

Strand: Reverse

Name: gadX [H]

Synonym: ECED1_4194

Alternate gene names: 218691800

Gene position: 4102295-4101471 (Counterclockwise)

Preceding gene: 218691801

Following gene: 218691799

Centisome position: 78.75

GC content: 41.33

Gene sequence:

>825_bases
ATGCAACCACTACATGGTAATTGTCTAATTGCGTACGCAAGACATAAATATATTCTCACCATGGTTAATGGTGAATATCG
CTATTTTAATGGCGGTGATCTGGTTTTTGCGGATGCAAGCCAAATTCAAGTGGATAAGTGTGTTGAGAATTTCGTATTAG
TTTCAAGAGACACGATTTCATTATTTCTCCCGATGCTCAAGGAGGAGGCATTAAAGCTTCATGTACATAAAAAAGTTCCT
TCATTACTCGTTCATCACTGTACCAGAGATATTCCTGTTTTTCAGGAAGTTGCGCAACTATCGCAGAATAAGAATCTTCG
CTATGCAGAAATGCTACGTAAAAGGGCATTAATCTTTGCGTTGTTATCTGTTTTTCTTGAGGATACGCAGTTTATACCGT
TACTTCTGAACGTGTTGCAACCGAATATGCGAACACGAGTTTGTACGGTCATCAATAATAATATCGCCCATGAGTGGACA
TTAGCCCGAATTGCTAGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTC
ACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTCAATTAAGCGAGTTGCAG
TGTCCTGTGGATATCACAGCGTGTCGTATTTCATTTACGTTTTTCGAAATTATTATGGGATGACGCCCACAGAGTATCAG
GAGCGATCGGCGCAGGAATTACCGAACTGTGGCCCGGCGGCAAGTATGGCTGCGCAAGGGATTTTTTACGGCACTGACCG
TTCTGCGGAAGGAATAAGATTATAG

Upstream 100 bases:

>100_bases
TTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAGTATGTT
TATGTAATATTAAGTCAACT

Downstream 100 bases:

>100_bases
AGTTTTACTCAGACATAAAAAAAACCCGGCATAGGGGACCGGGAAGAGGATAGTCTGCCGTCTCCAGACTAATAAACCGT
TATAACACTCCCTGTTGGCA

Product: DNA-binding transcriptional regulator GadX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTISLFLPMLKEEALKLHVHKKVP
SLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQELPNCGPAASMAAQGIFYGTDRSAEGIRL

Sequences:

>Translated_274_residues
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTISLFLPMLKEEALKLHVHKKVP
SLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQELPNCGPAASMAAQGIFYGTDRSAEGIRL
>Mature_274_residues
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTISLFLPMLKEEALKLHVHKKVP
SLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQELPNCGPAASMAAQGIFYGTDRSAEGIRL

Specific function: Positively regulates the expression of about fifteen genes involved in acid resistance such as gadA, gadB and gadC. Depending on the conditions (growth phase and medium), can repress gadW. Negatively regulates perA expression in acidic conditions and posi

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789933, Length=274, Percent_Identity=92.3357664233577, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1790557, Length=223, Percent_Identity=36.7713004484305, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786778, Length=194, Percent_Identity=39.6907216494845, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787776, Length=240, Percent_Identity=31.25, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786776, Length=215, Percent_Identity=31.1627906976744, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789932, Length=127, Percent_Identity=38.5826771653543, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 31317; Mature: 31317

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTIS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECCCHHHHHHHHHHHEEECCHHHH
LFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQELPNCGPAASMAAQGIFYGTDRSAEGIRL
HHHHHHCCCCCCHHHHHHCCEEECCCCCCCCCCC
>Mature Secondary Structure
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTIS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECCCHHHHHHHHHHHEEECCHHHH
LFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQELPNCGPAASMAAQGIFYGTDRSAEGIRL
HHHHHHCCCCCCHHHHHHCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA