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The map label for this gene is gadW [H]

Identifier: 218691799

GI number: 218691799

Start: 4100374

End: 4101102

Strand: Reverse

Name: gadW [H]

Synonym: ECED1_4193

Alternate gene names: 218691799

Gene position: 4101102-4100374 (Counterclockwise)

Preceding gene: 218691800

Following gene: 218691794

Centisome position: 78.72

GC content: 41.02

Gene sequence:

>729_bases
ATGGCTCATGTCTGCTCGGTGATCCTCGTTCGTCGTTCATTCGATATTCATCATGAACAGCAAAAAATATCGTTGCATAA
CGAGAGTATCCTGCTGCTGGATAAAAATTTGGCAGACGATTTTGCGTTTTGTTCACTGGATACGCGACAGCTGGATATCG
AAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGATTGCATAGCAAAGACCGTTTG
TTAATTAACCAGTCACCCCCCATACAGCTGGTGACGGCGATTTTTGATAGTTTCAATGACCCCCGGGTCAATTCGCCGAT
ACTGAGCAAAATGCTCTATCTTTCCTGTTTATCAATGTTTTCTCATAAGAAAGAACTGATCCCCTTACTTTTCAATAGTA
TCAGTACTGTTTCAGGAAAAGTTGAACGCCTTATTAGCTTTGATATCGCTAAACGTTGGTATCTACGCGATATCGCAGAA
AGAATGTACACCAGCGAGAGTCTCATCAAAAAAAAGTTGCAGGATGAAAATACCTGTTTCAGTAAAATATTACTCGCCTC
CAGGATGTCGATGGCCAGACGATTACTCGAGTTACGTCAAATACCTCTGCATACTATTGCGGAAAAATGTGGCTATAGCA
GTACGTCATACTTTATAAATACATTTAGACAATATTATGGTGTAACGCCACATCAGTTTTCGCAACATTCGCCGGGTACC
TTTTCCTGA

Upstream 100 bases:

>100_bases
TACCAAATATGGCAGTTTTTGCACACAGAAACAGTCTGGCGTCATTTCATTAGTATACTGACATTGAAATAATCGCAGTA
ATGAAATATAAGGGATAGTC

Downstream 100 bases:

>100_bases
CATATTTCGCATTTGAATATTGGTCAGGATCTCACGTCTGCTTCATGTGAAACTCTTTCCTGATGATTTCTGCCGGGCTA
CCGGCTAGTTCTCTTTCGCA

Product: DNA-binding transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFCSLDTRQLDIEELTVCHYLQNIRQLPRNLGLHSKDRL
LINQSPPIQLVTAIFDSFNDPRVNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFSQHSPGT
FS

Sequences:

>Translated_242_residues
MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFCSLDTRQLDIEELTVCHYLQNIRQLPRNLGLHSKDRL
LINQSPPIQLVTAIFDSFNDPRVNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFSQHSPGT
FS
>Mature_241_residues
AHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFCSLDTRQLDIEELTVCHYLQNIRQLPRNLGLHSKDRLL
INQSPPIQLVTAIFDSFNDPRVNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAER
MYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFSQHSPGTF
S

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789932, Length=242, Percent_Identity=94.2148760330578, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1786776, Length=241, Percent_Identity=34.8547717842324, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787776, Length=237, Percent_Identity=34.5991561181435, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1789933, Length=209, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790557, Length=136, Percent_Identity=37.5, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1786778, Length=243, Percent_Identity=30.8641975308642, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 28085; Mature: 27954

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFCSLDTRQLDIEELTVCH
CHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHH
YLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPRVNSPILSKMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFSQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHCCCCCCCC
FS
CC
>Mature Secondary Structure 
AHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFCSLDTRQLDIEELTVCH
HHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHH
YLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPRVNSPILSKMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFSQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHCCCCCCCC
FS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]