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The map label for this gene is slyD

Identifier: 218691629

GI number: 218691629

Start: 3915327

End: 3915917

Strand: Reverse

Name: slyD

Synonym: ECED1_4010

Alternate gene names: 218691629

Gene position: 3915917-3915327 (Counterclockwise)

Preceding gene: 218691630

Following gene: 218691627

Centisome position: 75.17

GC content: 55.16

Gene sequence:

>591_bases
ATGAAAGTAGCAAAAGACCTGGTGGTCAGCCTGGCCTATCAGGTACGTACAGAAGACGGTGTGTTGGTTGATGAGTCTCC
GGTGAGTGCGCCGCTGGACTACCTGCATGGTCACGGTTCCCTGATCTCTGGCCTGGAAACAGCGCTGGAAGGTCATGAAG
TTGGCGACAAATTTGATGTCGCTGTTGGCGCGAACGACGCTTACGGTCAGTACGACGAAAACCTGGTGCAGCGCGTTCCT
AAAGACGTATTTATGGGCGTTGATGAACTGCAGGTAGGTATGCGTTTCCTGGCTGAAACCGACCAGGGTCCGGTACCGGT
TGAAATCACTGCGGTTGAAGACGATCACGTAGTGGTTGATGGTAACCACATGCTGGCTGGTCAGAACCTGAAATTCAACG
TTGAAGTTGTGGCGATTCGCGAAGCGACCGAAGAAGAACTGGCTCATGGTCACGTTCACGGCGCGCACGATCACCACCAC
GATCACGACCACGACGGTTGCTGCGGTGGTCATGGCCACGATCACGGTCATGAACACGGTGGCGAAGGCTGCTGTGGCGG
TAAAGGCAACGGCGGTTGCGGTTGCCACTAA

Upstream 100 bases:

>100_bases
GACTAGCGATATGCGCCGAGTTTTTTTAAGCTAGTGAGTACACGGCTGCAGAATTCCGCTACAATCTGCGCCACTATTCT
TCCCATGCTCAGGAGATATC

Downstream 100 bases:

>100_bases
TACCGAAAAGGTGACAAAAAAGCGGGGAATCCCCGCTTTTTTTACGCCTCAATAATGTGGCGGTGGCGTTTCTTCAGCCT
GCGACGCGATGTTCGACGGC

Product: FKBP-type peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase; Metallochaperone slyD

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVP
KDVFMGVDELQVGMRFLAETDQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH

Sequences:

>Translated_196_residues
MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVP
KDVFMGVDELQVGMRFLAETDQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH
>Mature_196_residues
MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVP
KDVFMGVDELQVGMRFLAETDQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH

Specific function: Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity

COG id: COG1047

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerases 2

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1789748, Length=196, Percent_Identity=100, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1786211, Length=135, Percent_Identity=32.5925925925926, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SLYD_ECO57 (P0A9L1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85999
- PIR:   H91153
- RefSeq:   NP_289896.1
- RefSeq:   NP_312227.1
- ProteinModelPortal:   P0A9L1
- SMR:   P0A9L1
- MINT:   MINT-1223673
- PRIDE:   P0A9L1
- EnsemblBacteria:   EBESCT00000028636
- EnsemblBacteria:   EBESCT00000059009
- GeneID:   915947
- GeneID:   961279
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4707
- KEGG:   ecs:ECs4200
- GeneTree:   EBGT00050000009174
- HOGENOM:   HBG731200
- OMA:   HGHIHAH
- ProtClustDB:   PRK10737
- BioCyc:   ECOL83334:ECS4200-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001179
- PANTHER:   PTHR10516

Pfam domain/function: PF00254 FKBP_C

EC number: =5.2.1.8

Molecular weight: Translated: 20853; Mature: 20853

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALEGHEVGDKFDV
CCHHHHHHHHHHHHEECCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCEEEE
AVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQGPVPVEITAVEDDHVVVD
EECCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEC
GNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHHDHDHDGCCGGHGHDHGHEHG
CCEEEECCCEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEGCCGGKGNGGCGCH
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALEGHEVGDKFDV
CCHHHHHHHHHHHHEECCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCEEEE
AVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQGPVPVEITAVEDDHVVVD
EECCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEC
GNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHHDHDHDGCCGGHGHDHGHEHG
CCEEEECCCEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEGCCGGKGNGGCGCH
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796