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The map label for this gene is yfiE [H]

Identifier: 218690693

GI number: 218690693

Start: 2971289

End: 2972170

Strand: Reverse

Name: yfiE [H]

Synonym: ECED1_3008

Alternate gene names: 218690693

Gene position: 2972170-2971289 (Counterclockwise)

Preceding gene: 218690695

Following gene: 218690691

Centisome position: 57.05

GC content: 49.43

Gene sequence:

>882_bases
ATGGATCTGCGCCGTTTTATTACGCTTAAAACCGTGGTGGAAGAGGGTTCCTTTTTGCGAGCTTCGCAAAAATTGTGCTG
CACACAATCGACGGTGACTTTTCATATTCAGCAGCTTGAACAGGAGTTCTCGGTACAGTTGTTTGAGAAAATTGGTCGAC
GAATGTGCCTTACCCGCGAAGGAAAAAAGCTGCTGCCGCACATCTATGAACTTACCCGAGTGATGGATACGCTTCGTGAG
GCTGCTAAAAAAGAGTCGGATCCGGACGGGGAACTACGCGTTATTTCAGGCGAAACGTTGCTCTCTTACCGAATGCCGCA
GGTGTTGCAGCGTTTTCGGCAACGCGCTCCAAAAGTGCGTTTATCGTTGCAGTCGCTGAACTGTTACGTGATCCGTGATG
CGCTACTGAATGATGAGGCCGATGTCGGGGTTTTTTATCGCGTAGGGAATGATGATGCTCTGAACCGACGAGAGTTGGGT
GAACAACCGCTGGTGCTGGTGGCTTCACCGCAAATTGCAGATGTCGATTTTACTGAGCCGGGAAGACATAACGCCTGTAG
CTTTATTATCAACGAACCACAATGTGTCTTCCGGCAGATATTTGAGAGCACGCTGTGCCAGCGGCGGATCACGGTGGAAA
ACACCATTGAGCTTATAAGTATCGAAAGCATCAAGCGTTGTGTCGCGGCAAATATCGGAGTCAGTTACCTGCCGCGTTTT
GCGGTGGTAAAAGAGTTGGAGTGCGGAGAATTAATTGAATTGCCTTTTGGCGAACAGTCGCAAACCATTACGGCCATGTG
CGCTCACCATGCCGGAAAAGCGGTTAGCCCGGCGATGCACACTTTTATTCAGTGTGTTGAAGAGTGTTTTTTGCCGGGAT
AA

Upstream 100 bases:

>100_bases
GTTATCTCCTCTGTTTTGGTAGACAGTACCCGACACGGAACATTTAAAAAAATGAATGTTTTTACATTCTAAATTCGATA
TTTTCGATAGGTTTGGGGTT

Downstream 100 bases:

>100_bases
AAACAATGCCGGGCTTAAACCCGGCATTGAGATTACTCTTCTGTCGTTTGCGGTGGCACGCCCGTTCCGCTTGGTTTACG
GCGCTTACCAATATTTTTAG

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVISGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLCQRRITVENTIELISIESIKRCVAANIGVSYLPRF
AVVKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEECFLPG

Sequences:

>Translated_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVISGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLCQRRITVENTIELISIESIKRCVAANIGVSYLPRF
AVVKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEECFLPG
>Mature_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVISGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLCQRRITVENTIELISIESIKRCVAANIGVSYLPRF
AVVKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEECFLPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=293, Percent_Identity=97.6109215017065, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1788481, Length=303, Percent_Identity=25.4125412541254, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787806, Length=288, Percent_Identity=24.3055555555556, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786401, Length=313, Percent_Identity=26.8370607028754, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=23.321554770318, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=30, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=284, Percent_Identity=25.3521126760563, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788887, Length=300, Percent_Identity=24, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788296, Length=137, Percent_Identity=34.3065693430657, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787879, Length=299, Percent_Identity=25.0836120401338, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI87082024, Length=258, Percent_Identity=25.968992248062, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33299; Mature: 33299

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEE
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQPLVLVASPQIADVDFTEP
EEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEEEEECCCCCCCCCCCC
GRHNACSFIINEPQCVFRQIFESTLCQRRITVENTIELISIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AVVKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEECFLPG
HHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEE
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQPLVLVASPQIADVDFTEP
EEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEEEEECCCCCCCCCCCC
GRHNACSFIINEPQCVFRQIFESTLCQRRITVENTIELISIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AVVKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEECFLPG
HHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]