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The map label for this gene is emrB [H]

Identifier: 218689328

GI number: 218689328

Start: 1520816

End: 1522330

Strand: Direct

Name: emrB [H]

Synonym: ECED1_1549

Alternate gene names: 218689328

Gene position: 1520816-1522330 (Clockwise)

Preceding gene: 218689327

Following gene: 218689329

Centisome position: 29.19

GC content: 41.72

Gene sequence:

>1515_bases
ATGATGTCTATGCGTAAACATATTGCTTTCGCCAGTATGTGTATGGGATTATTTATTGCTCAGCTTGATATTCAAATTGT
ATCGTCATCCCTAAATGAAATAGGTGGTGGTCTGTCCGCAGGTAAAGACGAAATGGCCTGGCTTCAGACTAGCTATTTAA
TTGCTGAGATTATTGTTATTCCGCTCTCTGGGTGGCTGTCACGGGTATTTTCCACTCGTTGGCTTTTTACTCTATCCGCG
GGAATATTTACTTTAATGAGTATCGCGTGTGGACTTGCATGGAATATTCAGATCATGATTTTGTTTCGTGCTTTACAGGG
AGCTGCTGGCGCATCCATGATCCCTTTAGTTTTCACGACGGCGTTTATTTATTACCAGGGTAAAGAGTTGGGGTTGGCTG
CTGCGGTAGTCAGTGCGCTGGCCTCACTGTCACCGACGCTAGGGCCAACTCTGGGGGGCTGGATCACTGATAACCTCGAC
TGGCGATGGCTATTTTATATCAATATATTACCTGGCATATATTTAGTTTTGAGTATTCCTTTTCTGGTTAATTTTGATAA
ACCTGATCTCTCTTTACTGAAAGTTGCTGATTATCCGAGTATTATCCTGTTGGCCATGACACTGGGATGTCTGGAATATA
CCCTTGAAGAAGGAGCGCGCTGGGGATGGCTGGATGATAATACTATTTTGCTGACATCAGTTCTGGCATTGGTAAGTTTT
ATTTTATTTGCTGCGCGTACACTTACAATATCCAATCCGATAATGGATCTTAATGCTTTTAAGGATAAAAATTTCACACT
GGGATGTTTTTTCTCTTTTTCTGGAGGGGTTGGAATATTCTCTACTGTATATTTAATACCTGTGTTTTTGGGGCAGGTTC
GCGGATTAAATGCCGAAGAGATAGGTTTTGCCGTTTGCACAACAGGTATGTTCCAGTTATTTTCTGTTCCCTTTTATTTC
TGGTTAAGTAAAAAAATAAATCTGCGATGGCTGTTGATGGCGGGGCTGGGTGGATTTGTGTTTTCAATGTATCTTTTTAC
CCCTATAACCCACGAATGGGGATGGCAGGAGTTACTGTTTCCACAGGCAATTAGAGGGATTTCGCAGCAGTTTGCGATGG
CCCCCATCGTAACATTAACGCTGGGGGGAATTCCAAAAGAACGGCTGAAGTTAGCATCTGGTGTATTTAATTTAACACGT
AATCTTGGGGGCGCAATTGGTATTGCCCTGTGTGGCTCTATTCTCAATAATCGAACTAATTTTCATTTTTCGAGAATGAG
TGAAAAAATGGTTTCTGTTCCCCATACGGTGAATGACTTCATTTCCAGAAGTGCTTTGTTTTTTAATCGCAGTGGAAGTG
ACCAGACATCGGAAATACTGGCTTCAACCAAATTATTGAGTCAATTGATGCTGAGGGAGGCGCAAACTATGGCATTTTCG
GATACCTTTCTGCTAATCAGTGGTCTGTTATTTATCGCATTTTTGTTGGTTCCTGCTATGAATAAATCATCCTGA

Upstream 100 bases:

>100_bases
TTGTTCAGCGTGTCCCGGTACGGATTGCATTTGACGACGCAAAGGAATTAAAGCAACTTCTTCGCCCCGGACTGTCCGTC
ACCGTCAGTGTTGATGAGAG

Downstream 100 bases:

>100_bases
TCGGAGCCTGTGCTGAATTAAACTCGGGCTAAACTGTTCGATACTGCTAACAGGCTAGTATAAAATGCCCCGCCTGCGCA
GATACTCACAGGTATGAAGT

Product: putative drug resistance transporter EmrB/QacA subfamily

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSA
GIFTLMSIACGLAWNIQIMILFRALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
ILFAARTLTISNPIMDLNAFKDKNFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGMFQLFSVPFYF
WLSKKINLRWLLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTR
NLGGAIGIALCGSILNNRTNFHFSRMSEKMVSVPHTVNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
DTFLLISGLLFIAFLLVPAMNKSS

Sequences:

>Translated_504_residues
MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSA
GIFTLMSIACGLAWNIQIMILFRALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
ILFAARTLTISNPIMDLNAFKDKNFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGMFQLFSVPFYF
WLSKKINLRWLLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTR
NLGGAIGIALCGSILNNRTNFHFSRMSEKMVSVPHTVNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
DTFLLISGLLFIAFLLVPAMNKSS
>Mature_504_residues
MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSA
GIFTLMSIACGLAWNIQIMILFRALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
ILFAARTLTISNPIMDLNAFKDKNFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGMFQLFSVPFYF
WLSKKINLRWLLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTR
NLGGAIGIALCGSILNNRTNFHFSRMSEKMVSVPHTVNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
DTFLLISGLLFIAFLLVPAMNKSS

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=493, Percent_Identity=28.1947261663286, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1788710, Length=433, Percent_Identity=29.0993071593533, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1790195, Length=452, Percent_Identity=25.6637168141593, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081983, Length=423, Percent_Identity=24.5862884160757, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=25.5474452554745, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=194, Percent_Identity=24.2268041237113, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55538; Mature: 55538

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
PLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILFRALQGAAGASMIPLVFTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLDWRWLFYINILPGIYLVLSIP
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHHHHHHH
FLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
HHEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ILFAARTLTISNPIMDLNAFKDKNFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEE
HHHHHHHHEECCCCEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHH
IGFAVCTTGMFQLFSVPFYFWLSKKINLRWLLMAGLGGFVFSMYLFTPITHEWGWQELLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
PQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGGAIGIALCGSILNNRTN
HHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
FHFSRMSEKMVSVPHTVNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
CHHHHHHHHHHCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DTFLLISGLLFIAFLLVPAMNKSS
HHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
PLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILFRALQGAAGASMIPLVFTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLDWRWLFYINILPGIYLVLSIP
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHHHHHHH
FLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
HHEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ILFAARTLTISNPIMDLNAFKDKNFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEE
HHHHHHHHEECCCCEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHH
IGFAVCTTGMFQLFSVPFYFWLSKKINLRWLLMAGLGGFVFSMYLFTPITHEWGWQELLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
PQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGGAIGIALCGSILNNRTN
HHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
FHFSRMSEKMVSVPHTVNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
CHHHHHHHHHHCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DTFLLISGLLFIAFLLVPAMNKSS
HHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]