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The map label for this gene is rutA [H]

Identifier: 218688977

GI number: 218688977

Start: 1169321

End: 1170469

Strand: Reverse

Name: rutA [H]

Synonym: ECED1_1168

Alternate gene names: 218688977

Gene position: 1170469-1169321 (Counterclockwise)

Preceding gene: 218688979

Following gene: 218688976

Centisome position: 22.47

GC content: 55.0

Gene sequence:

>1149_bases
ATGCAGGATGCAGCGCCCCGTCTGACTTTCACTTTACGAGACGAAGAGAGGTTGATGATGAAAATTGGCGTATTCGTACC
TATTGGCAACAACGGCTGGCTCATTTCGACCCACGCGCCGCAGTACATGCCGACCTTTGAACTGAATAAAGCCATTGTGC
AAAAAGCGGAGCACTACCATTTCGATTTCGCCCTGTCGATGATCAAACTGCGTGGCTTTGGCGGCAAAACTGAGTTCTGG
GATCACAACCTTGAGTCGTTCACCTTGATGGCGGGGCTGGCAGCCGTCACCTCACGCATTCAGATATACGCGACAGCTGC
CACCTTAACGTTACCTCCGGCAATCGTCGCCCGTATGGCCGCAACCATCGACTCCATCTCTGGCGGGCGTTTTGGCGTCA
ACCTCGTGACTGGCTGGCAGAAGCCCGAGTATGAGCAGATGGGGATCTGGCCTGGCGATGACTATTTCTCCCGTCGTTAC
GACTATCTCACCGAATATGTTCAGGTGCTGCGCGACCTGTGGGGCTCGGGGAAAAGCGATTTTAAAGGCGATTTTTTCAC
TATGGATGATTGTCGCGTCAGTCCGCAACCGAGTGTCCCCATGAAAGTGATCTGCGCCGGGCAAAGCGACGCTGGCATGG
CGTTCTCCGCCCGGTATGCCGATTTTAACTTCTGTTTCGGCAAAGGCGTAAATACACCCACGGCTTTCGCCCCGACCGCT
GCGCGGATGAAACAGGCCGCAGAGCAAACCGGACGCGACGTTGGCTCTTATGTGTTGTTTATGGTGATTGCCGACGAAAC
CGACGATGCCGCTCGTGCAAAATGGGAACACTACAAAGCGGGCGCGGATGAAGAGGCGTTAAGCTGGCTAACCGAACAAA
GTCAGAAAGATACCCGCTCCGATACTGACACCAACGTCCGTCAGATGGCCGATCCCACTTCGGCGGTAAACATCAATATG
GGGACGTTAGTCGGTTCTTACGCCAGTGTCGCGCGCATGTTAGATGAAGTCGCAAGCGTGCCTGGTGCCGAAGGCGTGCT
GTTGACCTTCGATGATTTTCTGTCGGGAATCGAAAACTTCGGCGAGCGCATTCAACCACTGATGCAGTGCCGCGCCCATC
TCCCTGCGCTGACTCAGGAGGTGGCATGA

Upstream 100 bases:

>100_bases
TTTTTTCAGGCATTTTAGACAACTTAACATATTGATAACATTCACTTTTTAAAAACTGGCATCCGCTTTGCAAACAAGCC
ATTACGCGGCTGAAAAGGAT

Downstream 100 bases:

>100_bases
TGACGACCTTAACCGCACGACCGGAAGCCATTACCTTCGATCCGCAGCAAACTGCGCTGATCGTGGTGGATATGCAAAAC
GCTTATGCCACGCCAGGCGG

Product: alternative pyrimidine degradation pathway monooxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIKLRGFGGKTEFW
DHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRY
DYLTEYVQVLRDLWGSGKSDFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFAPTA
ARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSDTDTNVRQMADPTSAVNINM
GTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIENFGERIQPLMQCRAHLPALTQEVA

Sequences:

>Translated_382_residues
MQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIKLRGFGGKTEFW
DHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRY
DYLTEYVQVLRDLWGSGKSDFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFAPTA
ARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSDTDTNVRQMADPTSAVNINM
GTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIENFGERIQPLMQCRAHLPALTQEVA
>Mature_382_residues
MQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIKLRGFGGKTEFW
DHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRY
DYLTEYVQVLRDLWGSGKSDFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFAPTA
ARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSDTDTNVRQMADPTSAVNINM
GTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIENFGERIQPLMQCRAHLPALTQEVA

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787247, Length=382, Percent_Identity=98.9528795811518, Blast_Score=790, Evalue=0.0,
Organism=Escherichia coli, GI1787166, Length=305, Percent_Identity=24.2622950819672, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: NA

Molecular weight: Translated: 42277; Mature: 42277

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYH
CCCCCCCEEEEECCHHHEEEEEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
FDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMA
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEEECCHHHHHHHH
ATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKSD
HHHHCCCCCEEEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFAPTA
CCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHH
ARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRS
HHHHHHHHHHCCHHHCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
DTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIENF
CCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
GERIQPLMQCRAHLPALTQEVA
HHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure
MQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYH
CCCCCCCEEEEECCHHHEEEEEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
FDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMA
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEEECCHHHHHHHH
ATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKSD
HHHHCCCCCEEEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFAPTA
CCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHH
ARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRS
HHHHHHHHHHCCHHHCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
DTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIENF
CCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
GERIQPLMQCRAHLPALTQEVA
HHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA