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The map label for this gene is rutB [H]

Identifier: 218688976

GI number: 218688976

Start: 1168629

End: 1169321

Strand: Reverse

Name: rutB [H]

Synonym: ECED1_1167

Alternate gene names: 218688976

Gene position: 1169321-1168629 (Counterclockwise)

Preceding gene: 218688977

Following gene: 218688975

Centisome position: 22.45

GC content: 55.41

Gene sequence:

>693_bases
ATGACGACCTTAACCGCACGACCGGAAGCCATTACCTTCGATCCGCAGCAAACTGCGCTGATCGTGGTGGATATGCAAAA
CGCTTATGCCACGCCAGGCGGCTACTTAGATCTCGCCGGGTTTGATGTCTCAACCACTCGCCCGGTCATTGCCAACATTC
AAACCGCCGTGACCGCAGCGCGAGCGGCAGGGATGTTGATCATCTGGTTTCAAAATGGCTGGGATGAACAGTATGTCGAG
GCTGGCGGCCCCGGCTCACCGAATTTTCATAAATCGAACGCCCTGAAAACCATGCGTAAGCAGCCGCAGCTGCAGGGGAA
ATTGCTGGCGAAAGGCTCCTGGGATTATCAACTGGTGGATGAACTGGTGCCGCAGCCTGGCGATATTGTGCTGCCGAAGC
CGCGCTACAGCGGTTTCTTCAATACGCCGCTGGACAGCATTTTGCGCAGCCGTGGAATACGCCATCTGGTTTTCACCGGT
ATCGCTACCAACGTCTGCGTCGAATCGACGCTACGCGACGGCTTTTTTCTGGAGTATTTCGGCGTGGTGCTGGAAGACGC
AACACACCAGGCGGGGCCGGAATTTGCACAGAAAGCCGCGTTGTTCAATATCGAAACCTTTTTTGGCTGGGTCAGCGACG
TCGAAACGTTCTGCGACGCGCTTTCTTCCACGTCCTTTGCTCGTATCGCTTAA

Upstream 100 bases:

>100_bases
TCGATGATTTTCTGTCGGGAATCGAAAACTTCGGCGAGCGCATTCAACCACTGATGCAGTGCCGCGCCCATCTCCCTGCG
CTGACTCAGGAGGTGGCATG

Downstream 100 bases:

>100_bases
GGAGTTTAACGATGCCAAAATCCGTAATTATTCCTGCTGGCAGCAGCGCACCGCTGGCCCCCTTCGTTCCCGGCACGCTG
GCTGATGGCGTGGTGTATGT

Product: enzyme of the alternative pyrimidine degradation pathway

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVE
AGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSSTSFARIA

Sequences:

>Translated_230_residues
MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVE
AGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSSTSFARIA
>Mature_229_residues
TTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEA
GGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGI
ATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSSTSFARIA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=230, Percent_Identity=98.2608695652174, Blast_Score=469, Evalue=1e-134,
Organism=Escherichia coli, GI87081992, Length=211, Percent_Identity=28.9099526066351, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 25233; Mature: 25102

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAA
CCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVD
HHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYF
HHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHHH
GVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSSTSFARIA
HHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAA
CCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVD
HHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYF
HHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHHH
GVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSSTSFARIA
HHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA