Click here to switch to the map view.

The map label for this gene is recE [H]

Identifier: 218688899

GI number: 218688899

Start: 1105316

End: 1107388

Strand: Reverse

Name: recE [H]

Synonym: ECED1_1090

Alternate gene names: 218688899

Gene position: 1107388-1105316 (Counterclockwise)

Preceding gene: 218688900

Following gene: 218688898

Centisome position: 21.26

GC content: 50.8

Gene sequence:

>2073_bases
ATGAGCGCTGATAAACAGACTTTTGCACTACACTGCGAAGCAAAAAACGATAAAGTCAGAAAACGCCTTGGCATCAAAGG
CGGTTTTTTCTGGACCGAGGCCAGAAAACTTTCTGTCGCAGTTTCCCGCTGCATTGCAGCCATGGACGATGCAGGCTACG
ACGAGGATGATTTCAAAAAACCCGTTCGCGTAAATTTCCCCGTCGTGAATGACCTTCCACCGGAAGGCGTGTTTGATACT
GAATTCTGCAACCGCTATGAAAAAGGCGGTAACGATGGCATCACCATGATGGCTATCCCCTTCGATGACAACATCAACGG
TGAAGATGCCACAACTGCTGGCGATGACAACGATAACCTGGACGGAACTATTCCGGATGATGTGGAGAAAAGCGAATCCC
CGGACAGCGACGATGACTGTTCTGAGTGTGAAATTCCCGTCGCCACTCTGAGCCTTACTCATCGCTTCCTTCACCTCTTC
TTATTCAGCAAAGATGAAGATGGAAAATACCGGCATCATGCCACACCAGAACAACGCAATAACGTGATCCGTATGGAGAT
GGACACAGAGGACAGTTACCTTCAGAGCCTGCTTACTGCTGTGCGCGCCGCGCATCATGAACTGGATAAACTGACGAACT
ATCACCTTAGTCGCCTGGCTGAATCTGTAGGGAAAGCATTCCCCCACTCTGCAAATCATCGCATCAGCCCGGCTGAATTC
GACAAGTTCATCTCCACCTGGATGAAAACAGACTATGTGGATCAGGGATTGCTGGCAAAAGAATGGCAGAAAGGAAATTA
TGTTACAGGCATCACTCGTACGCCTTCCGGTGCTAACGCTGGCGGCGGAAATCTTACCGATCGTGGCGAAGGCTTTACCC
ACAATCAGGCATCACTGGCGCGAGACATTGCCACTGGCGTTCTGGCCCGTTCAATGGATGTGGATATTTATAACCTGCAC
CCAGCACACGCAAAACGCGTTGAAGAAATCGTGTCAGAGAATAAGCCGCCCTTTTCTGTTTTCCGTGACAAATTCATCGC
CATGCCCGGTGGGCTGGATTATTCCCGCGCCATTGTGGTGGCTTCAGTGAAAGAAGCACCAATCGGCATTGAGGCTATCC
CGGCGCGCGTGACTGAATATCTCAACAAAGTGTTGACCGAAACCGATCACTCTAACCCGGATCCGGAAATCGTGGAAATC
GCCTGCGGTCGCTCATCAGCCCCAATGCCGCAGCGCGGAACAGCAGAAGAGATACATGACGATGAAGAAAAGCAGCAAAC
ATCGGACGCAATGTCTAATGAACAGGCAGCGCCTGAATCAGTGGAAGAAATTCCAGTTAAACATAATGCGGACACGCAAT
CACTGGAAAATGTCTCATCTGTAGAAACGAAATACCAGGAACTGAGGGAGGAACTCAATAAAGCCAGGGAAAACATCCCC
CCAAAAAATCCAGTCGATGCCGACAAATTACTGGCTGCCTCGCGTGGAGAATTCGTTGAAGGCATCAGTAACCCTGCTGA
TCCGAAGTGGGTGAAGGGGATCCAGACTCGCGACACTGAGGACCAGAATCAGTCCAAAGTGGAACAAATTGCCCCAGAAG
CGGGACAAAACAGCCCGGATACGCAACAAAACGGGCCAGAAGAGCAACAGCCAGGGCCAGTAGCGCAACCGGAGCTGGAA
AAAAACTGCCGCGTCTGTGGTCAGACTGGCGGGGGTAACTGCCCTGACTGTAGTGCGGTAATGGGCGATAGCACTTACAC
AGAAACTTTTGGAGAAAATGACGCCGCTGATGGAGAAGACTCTGCACAAACTGAGGAGAAGATCATTCAGGAAAACTCTG
TTGATGCCGCTCAGGAGGGCGAAACCGTTGTTCAGAACGAGCCAGGCAGTGATACGTCCGGCGATGACGCCAATTCTGAG
CCAGTAACTCTCGACTGGAAAAGACAGCTCGTGATTGCCGCCGTCTATGGTTTGTGCGCCAACCCCGCATGTATAGCCAC
AGCGCCAGCAATCCCTGATATCGCCATCATGATTGCCAACAGGCTTGAAAATTTCGGAGGTGATAAATCATGA

Upstream 100 bases:

>100_bases
ATCCGGCAGCGCAGGCTACCGGATTTCTCCCTGCGTCACCGTATTCGGAGAAATCAGACAAAGGGCCGCTAATTCTAATC
CAGCCAGAGGTTTAAATACA

Downstream 100 bases:

>100_bases
ATGCCTGGCTTATCCCCGATCGCATTGAAGAGCAGTCATGGGCACGACACTACCAGCAAATTGCCCGTGAAGAAACTGAA
GCTGAGCTGGCAGACGACCT

Product: Exodeoxyribonuclease VIII (putative partial) from phage origin

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MSADKQTFALHCEAKNDKVRKRLGIKGGFFWTEARKLSVAVSRCIAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDT
EFCNRYEKGGNDGITMMAIPFDDNINGEDATTAGDDNDNLDGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLF
LFSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRAAHHELDKLTNYHLSRLAESVGKAFPHSANHRISPAEF
DKFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPSGANAGGGNLTDRGEGFTHNQASLARDIATGVLARSMDVDIYNLH
PAHAKRVEEIVSENKPPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLNKVLTETDHSNPDPEIVEI
ACGRSSAPMPQRGTAEEIHDDEEKQQTSDAMSNEQAAPESVEEIPVKHNADTQSLENVSSVETKYQELREELNKARENIP
PKNPVDADKLLAASRGEFVEGISNPADPKWVKGIQTRDTEDQNQSKVEQIAPEAGQNSPDTQQNGPEEQQPGPVAQPELE
KNCRVCGQTGGGNCPDCSAVMGDSTYTETFGENDAADGEDSAQTEEKIIQENSVDAAQEGETVVQNEPGSDTSGDDANSE
PVTLDWKRQLVIAAVYGLCANPACIATAPAIPDIAIMIANRLENFGGDKS

Sequences:

>Translated_690_residues
MSADKQTFALHCEAKNDKVRKRLGIKGGFFWTEARKLSVAVSRCIAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDT
EFCNRYEKGGNDGITMMAIPFDDNINGEDATTAGDDNDNLDGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLF
LFSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRAAHHELDKLTNYHLSRLAESVGKAFPHSANHRISPAEF
DKFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPSGANAGGGNLTDRGEGFTHNQASLARDIATGVLARSMDVDIYNLH
PAHAKRVEEIVSENKPPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLNKVLTETDHSNPDPEIVEI
ACGRSSAPMPQRGTAEEIHDDEEKQQTSDAMSNEQAAPESVEEIPVKHNADTQSLENVSSVETKYQELREELNKARENIP
PKNPVDADKLLAASRGEFVEGISNPADPKWVKGIQTRDTEDQNQSKVEQIAPEAGQNSPDTQQNGPEEQQPGPVAQPELE
KNCRVCGQTGGGNCPDCSAVMGDSTYTETFGENDAADGEDSAQTEEKIIQENSVDAAQEGETVVQNEPGSDTSGDDANSE
PVTLDWKRQLVIAAVYGLCANPACIATAPAIPDIAIMIANRLENFGGDKS
>Mature_689_residues
SADKQTFALHCEAKNDKVRKRLGIKGGFFWTEARKLSVAVSRCIAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDTE
FCNRYEKGGNDGITMMAIPFDDNINGEDATTAGDDNDNLDGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLFL
FSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRAAHHELDKLTNYHLSRLAESVGKAFPHSANHRISPAEFD
KFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPSGANAGGGNLTDRGEGFTHNQASLARDIATGVLARSMDVDIYNLHP
AHAKRVEEIVSENKPPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLNKVLTETDHSNPDPEIVEIA
CGRSSAPMPQRGTAEEIHDDEEKQQTSDAMSNEQAAPESVEEIPVKHNADTQSLENVSSVETKYQELREELNKARENIPP
KNPVDADKLLAASRGEFVEGISNPADPKWVKGIQTRDTEDQNQSKVEQIAPEAGQNSPDTQQNGPEEQQPGPVAQPELEK
NCRVCGQTGGGNCPDCSAVMGDSTYTETFGENDAADGEDSAQTEEKIIQENSVDAAQEGETVVQNEPGSDTSGDDANSEP
VTLDWKRQLVIAAVYGLCANPACIATAPAIPDIAIMIANRLENFGGDKS

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=551, Percent_Identity=52.2686025408348, Blast_Score=513, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 75432; Mature: 75301

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADKQTFALHCEAKNDKVRKRLGIKGGFFWTEARKLSVAVSRCIAAMDDAGYDEDDFKK
CCCCCCEEEEEEECCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
PVRVNFPVVNDLPPEGVFDTEFCNRYEKGGNDGITMMAIPFDDNINGEDATTAGDDNDNL
CEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
DGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLFLFSKDEDGKYRHHATPEQRN
CCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC
NVIRMEMDTEDSYLQSLLTAVRAAHHELDKLTNYHLSRLAESVGKAFPHSANHRISPAEF
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
DKFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPSGANAGGGNLTDRGEGFTHNQASLA
HHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
RDIATGVLARSMDVDIYNLHPAHAKRVEEIVSENKPPFSVFRDKFIAMPGGLDYSRAIVV
HHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEE
ASVKEAPIGIEAIPARVTEYLNKVLTETDHSNPDPEIVEIACGRSSAPMPQRGTAEEIHD
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHCC
DEEKQQTSDAMSNEQAAPESVEEIPVKHNADTQSLENVSSVETKYQELREELNKARENIP
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKNPVDADKLLAASRGEFVEGISNPADPKWVKGIQTRDTEDQNQSKVEQIAPEAGQNSPD
CCCCCCHHHHHHHHCCHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
TQQNGPEEQQPGPVAQPELEKNCRVCGQTGGGNCPDCSAVMGDSTYTETFGENDAADGED
CCCCCCCCCCCCCCCCCCHHCCCCEECCCCCCCCCCCHHHHCCCHHHHHCCCCCCCCCCC
SAQTEEKIIQENSVDAAQEGETVVQNEPGSDTSGDDANSEPVTLDWKRQLVIAAVYGLCA
HHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHC
NPACIATAPAIPDIAIMIANRLENFGGDKS
CCCCEEECCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SADKQTFALHCEAKNDKVRKRLGIKGGFFWTEARKLSVAVSRCIAAMDDAGYDEDDFKK
CCCCCEEEEEEECCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
PVRVNFPVVNDLPPEGVFDTEFCNRYEKGGNDGITMMAIPFDDNINGEDATTAGDDNDNL
CEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
DGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLFLFSKDEDGKYRHHATPEQRN
CCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC
NVIRMEMDTEDSYLQSLLTAVRAAHHELDKLTNYHLSRLAESVGKAFPHSANHRISPAEF
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
DKFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPSGANAGGGNLTDRGEGFTHNQASLA
HHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
RDIATGVLARSMDVDIYNLHPAHAKRVEEIVSENKPPFSVFRDKFIAMPGGLDYSRAIVV
HHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEE
ASVKEAPIGIEAIPARVTEYLNKVLTETDHSNPDPEIVEIACGRSSAPMPQRGTAEEIHD
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHCC
DEEKQQTSDAMSNEQAAPESVEEIPVKHNADTQSLENVSSVETKYQELREELNKARENIP
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKNPVDADKLLAASRGEFVEGISNPADPKWVKGIQTRDTEDQNQSKVEQIAPEAGQNSPD
CCCCCCHHHHHHHHCCHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
TQQNGPEEQQPGPVAQPELEKNCRVCGQTGGGNCPDCSAVMGDSTYTETFGENDAADGED
CCCCCCCCCCCCCCCCCCHHCCCCEECCCCCCCCCCCHHHHCCCHHHHHCCCCCCCCCCC
SAQTEEKIIQENSVDAAQEGETVVQNEPGSDTSGDDANSEPVTLDWKRQLVIAAVYGLCA
HHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHC
NPACIATAPAIPDIAIMIANRLENFGGDKS
CCCCEEECCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]