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The map label for this gene is cusS [H]

Identifier: 218688396

GI number: 218688396

Start: 583513

End: 584955

Strand: Reverse

Name: cusS [H]

Synonym: ECED1_0562

Alternate gene names: 218688396

Gene position: 584955-583513 (Counterclockwise)

Preceding gene: 218688397

Following gene: 218688395

Centisome position: 11.23

GC content: 51.98

Gene sequence:

>1443_bases
ATGGTCAGTAAGCCATTTCAGCGCCCGTTTTCGCTGGCAACCCGCCTGACCTTTTTTATCAGCCTCGCCACCATCGCGGC
GTTTTTCGCCTTTGCATGGATCATGATCCACTCGGTAAAAGTGCATTTTGCCGAGCAGGATATTAATGATTTAAAGGAGA
TTAGCGCCACCCTTGAACGGGTACTTAATCACCCTGACGAAACGCAAGCCCGACGCTTAATGACGCTGGAAGATATCGTC
AGTGGTTATTCCAATGTGTTGATCTCCCTGGCAAATAGCCACGGTAAAACGGTGTATCACTCTCCAGGTGCGCCGGATAT
CCGCGAGTTTACGCGTGACGCCATACCCGATAAAGACGCGCAGGCTGGCGAGGTGTATCTCCTTTCCGGCCCAACGATGA
TGATGCCAGGGCACGGTCACGGGCATATGGAACACAGCAACTGGCGGATGATTAACTTGCCGGTTGGCCCGCTGGTTGAC
GGCAAACCGATTTATACGCTCTACATCGCGCTTTCGATCGATTTTCATCTTCATTACATCAATGATTTGATGAATAAACT
TATTATGACCGCATCGGTAATCAGCATCCTGATCGTCTTTATCGTGCTGTTGGCGGTACATAAAGGTCACGCGCCGATCC
GCAGCGTCAGCCGTCAAATCCAGAATATCACCTCGAAAGATCTCGACGTTCGCCTTGACCCGCAGACCGTGCCCATTGAG
CTGGAACAGCTGGTACTGTCGTTCAACCATATGATCGAGCGTATTGAGGATGTCTTTACCCGCCAGTCCAATTTCTCAGC
GGATATCGCCCACGAAATTCGCACACCTATTACTAATCTCATTACGCAAACGGAAATCGCCCTCAGCCAGTCGCGCAGCC
AGAAGGAGCTGGAAGATGTGCTCTACTCTAATCTCGAAGAGCTGACGCGAATGGCGAAAATGGTCAGCGATATGCTGTTT
CTCGCTCAGGCCGATAACAACCAGCTAATCCCCGAAAAGAAAATGCTCAACCTGGCGGATGAAGTCGGCAAAGTGTTCGA
TTTTTTCGAGGCGTTAGCAGAAGATCGCGGCGTGGAGCTGCGGTTTGTTGGCGACGAGTGTCAGGTTGCGGGCGATCCGC
TGATGCTGCGTCGGGCGTTAAGCAACCTGCTTTCTAATGCCCTGCGTTATACGCCAACCGGAGAGACAATTGTCGTGCGC
TGCCAGACGGTCGATCACCTGGTGCAAGTTACCGTCGAAAACCCCGGTACGCCCATTGCGCCCGAACACTTACCGCGATT
GTTTGACCGTTTCTACCGCGTTGATCCTTCCCGCCAGCGAAAAGGCGAAGGTAGCGGCATTGGCCTGGCGATAGTGAAAT
CGATTGTTGTCGCGCATAAAGGCACAGTTGCAGTAACGTCAGATGTGCGGGGGACAAGGTTTGTTATCATATTACCCGCT
TAA

Upstream 100 bases:

>100_bases
GATGTGGCGGTGAAGCGGCTGCGCGGCAAAATCGACAACGACTTTGAGCCAAAGTTGATTCAGACCGTGCGCGGCGTGGG
TTACATGCTAGAGGTGCCGG

Downstream 100 bases:

>100_bases
TCCTTCAGCAAACGGCAACTTTTATAACCAGTGTAAAAATAACATGCCGCAATAGTAATACCTATTAATGATAAAAAGCT
GTCACAATTCATAAAAAACC

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLANSHGKTVYHSPGAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVR
CQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDVRGTRFVIILPA

Sequences:

>Translated_480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLANSHGKTVYHSPGAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVR
CQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDVRGTRFVIILPA
>Mature_480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLANSHGKTVYHSPGAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVR
CQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDVRGTRFVIILPA

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=480, Percent_Identity=98.5416666666667, Blast_Score=972, Evalue=0.0,
Organism=Escherichia coli, GI1788279, Length=296, Percent_Identity=33.445945945946, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786600, Length=263, Percent_Identity=33.8403041825095, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=30.6273062730627, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790346, Length=332, Percent_Identity=30.1204819277108, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.5819935691318, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789403, Length=282, Percent_Identity=31.2056737588652, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=27.4261603375527, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790551, Length=298, Percent_Identity=28.1879194630872, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789808, Length=283, Percent_Identity=27.5618374558304, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.5423728813559, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081816, Length=251, Percent_Identity=27.0916334661355, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=25.9090909090909, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=28.695652173913, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1787374, Length=347, Percent_Identity=23.9193083573487, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=292, Percent_Identity=25.6849315068493, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI48994928, Length=240, Percent_Identity=25.8333333333333, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87082128, Length=296, Percent_Identity=24.3243243243243, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=25.6198347107438, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53790; Mature: 53790

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSPGAPDIREFTRDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCCC
QAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEHHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVRCQTVDHLVQVTVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDVRGTRFVIILPA
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC
>Mature Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSPGAPDIREFTRDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCCC
QAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEHHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVRCQTVDHLVQVTVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDVRGTRFVIILPA
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]