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The map label for this gene is thiL [H]

Identifier: 218688280

GI number: 218688280

Start: 456481

End: 457458

Strand: Direct

Name: thiL [H]

Synonym: ECED1_0440

Alternate gene names: 218688280

Gene position: 456481-457458 (Clockwise)

Preceding gene: 218688279

Following gene: 218688281

Centisome position: 8.76

GC content: 54.29

Gene sequence:

>978_bases
ATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCAT
CGGCGACGATTGCGCTCTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGCAACC
ACTTCCTCCCTGATATCGATCCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGG
GCCGATCCAGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTTGACGAAGCGTGGCTTGAGTCCTTCAGCGACAGTTT
GTTTGATCTTCTCAATTATTACGATATGCAACTCATTGGCGGCGATACCACGCGTGGGCCATTATCAATGACGTTGGGTA
TCCACGGTTTTGTTCCGATGGGACGAGCCTTAACGCGCTCTGGGGCGAAACCGGGTGACTGGATCTATGTGACCGGTACA
CCGGGCGATAGCGCCGCCGGACTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGAT
CAAACGTCATCTCCGTCCATCTCCGCGTATTTTACAGGGACAGGCACTGCGCGATCTGGCAAATTCAGCTATCGATCTCT
CTGACGGTCTGATTTCCGATCTCGGGCATATCGTGAAAGCCAGCGACTGCGGCGCACGTATTGACCTGGCATTGCTGCCG
TTTTCTGATGCGCTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTT
GTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCG
GGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTCACGTTAGACTGGAAAGGATATGAC
CATTTTGCCACGCCATAA

Upstream 100 bases:

>100_bases
TGATTCGCCCTAACAAAAAGTAATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTT
TACCTGCTGAGGCATAACGT

Downstream 100 bases:

>100_bases
AGATGTCGCGAAAAGTCGCCTGAAAATGAGTAATCCGTGGCATCTGCTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGA
TCGTTCCTGGCACGATGGGG

Product: thiamine monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMG
ADPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLP
FSDALSRHVEPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYD
HFATP

Sequences:

>Translated_325_residues
MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMG
ADPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLP
FSDALSRHVEPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYD
HFATP
>Mature_324_residues
ACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGA
DPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGTP
GDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLPF
SDALSRHVEPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDH
FATP

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=325, Percent_Identity=100, Blast_Score=655, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 35071; Mature: 34940

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDID
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCEEEEECCEEEECCCCCCCCC
PADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIG
HHHHHHHHHHCCHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEC
GDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGTPGDSAAGLAILQNRLQVADA
CCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC
KDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLP
CCHHHHHHHHCCCCCCEECCHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCEEEEEEEC
FSDALSRHVEPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADI
CHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEECCHHCCC
EGLCFIRDGEPVTLDWKGYDHFATP
CEEEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
ACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDID
CCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCEEEEECCEEEECCCCCCCCC
PADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLESFSDSLFDLLNYYDMQLIG
HHHHHHHHHHCCHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEC
GDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGTPGDSAAGLAILQNRLQVADA
CCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC
KDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLISDLGHIVKASDCGARIDLALLP
CCHHHHHHHHCCCCCCEECCHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCEEEEEEEC
FSDALSRHVEPEQALRWALSGGEDYELCFTVPELNRGALDVALGHLGVPFTCIGQMTADI
CHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEECCHHCCC
EGLCFIRDGEPVTLDWKGYDHFATP
CEEEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]