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The map label for this gene is ribH

Identifier: 218688278

GI number: 218688278

Start: 455494

End: 455964

Strand: Direct

Name: ribH

Synonym: ECED1_0438

Alternate gene names: 218688278

Gene position: 455494-455964 (Clockwise)

Preceding gene: 218688277

Following gene: 218688279

Centisome position: 8.74

GC content: 52.02

Gene sequence:

>471_bases
ATGAACATTATTGAAGCTAACGTTGCTACCCCGGACGCTCGCGTCGCCATCACCATCGCGCGTTTCAACAACTTTATCAA
TGACAGCCTGCTGGAAGGTGCAATTGACGCACTGAAACGTATCGGTCAGGTAAAAGATGAAAACATTACCGTTGTTTGGG
TGCCAGGTGCCTATGAGCTGCCGCTGGCGGCGGGTGCACTGGCTAAAACCGGTAAATACGACGCGGTGATTGCGCTGGGT
ACGGTTATTCGTGGTGGCACTGCCCACTTTGAATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGA
CAGCGAAATTCCGGTTGCTTTTGGTGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTG
GCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGA

Upstream 100 bases:

>100_bases
GTGGGGGCATGATCTCCCGGCTCGAAAGGGAAGCAGCGCACGAAATATTATGCTAAAATCCGCCCCCCTGCGGGGCCATA
CTCGAACCCGAAGGAAGAAA

Downstream 100 bases:

>100_bases
AATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGC
AGTTGTCCCAGAACGACATC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQVKDENITVVWVPGAYELPLAAGALAKTGKYDAVIALG
TVIRGGTAHFEYVAGGASNGLAHVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA

Sequences:

>Translated_156_residues
MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQVKDENITVVWVPGAYELPLAAGALAKTGKYDAVIALG
TVIRGGTAHFEYVAGGASNGLAHVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA
>Mature_156_residues
MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQVKDENITVVWVPGAYELPLAAGALAKTGKYDAVIALG
TVIRGGTAHFEYVAGGASNGLAHVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=156, Percent_Identity=100, Blast_Score=308, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6324429, Length=143, Percent_Identity=39.1608391608392, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_CITK8 (A8AK39)

Other databases:

- EMBL:   CP000822
- RefSeq:   YP_001454288.1
- ProteinModelPortal:   A8AK39
- SMR:   A8AK39
- STRING:   A8AK39
- GeneID:   5583467
- GenomeReviews:   CP000822_GR
- KEGG:   cko:CKO_02746
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16157; Mature: 16157

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQVKDENITVVWVPGAYEL
CCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLAHVAQDSEIPVAFGVLTTE
CHHHHHHHCCCCCHHHEEEHHHHCCCCCEEEEEECCCCCCCEECCCCCCCCEEEEHCCHH
SIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQVKDENITVVWVPGAYEL
CCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLAHVAQDSEIPVAFGVLTTE
CHHHHHHHCCCCCHHHEEEHHHHCCCCCEEEEEECCCCCCCEECCCCCCCCEEEEHCCHH
SIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA