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The map label for this gene is proY

Identifier: 218688265

GI number: 218688265

Start: 441972

End: 443345

Strand: Direct

Name: proY

Synonym: ECED1_0425

Alternate gene names: 218688265

Gene position: 441972-443345 (Clockwise)

Preceding gene: 218688264

Following gene: 218688266

Centisome position: 8.48

GC content: 52.26

Gene sequence:

>1374_bases
ATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGACACATACGCTTTATGGCACTGGGTTCAGCAATTGGCAC
TGGGCTGTTTTACGGTTCGGCAGATGCCATCAAAATGGCCGGTCCAAGCGTGTTGTTGGCCTATATTATCGGTGGTATCG
CGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAG
GAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTTATTGTCGCCATCGCCGATGT
GACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGTGTGGTGCTGATCA
TTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAGTTCTGGTTCTCGTTCTTTAAAGTCGCCACTATC
ATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCT
GTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGTATGGTGATGTCGTTGCAAATGGTGATGTTTGCTTACGGTG
GGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATTCCGCGTGCGATTAACTCCGTGCCG
ATGCGTATTCTGGTGTTCTACGTCGGTACGTTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGG
TAGCCCGTTTGTGCTGACGTTCCAGCATATGGGCATTACCTTTGCCGCCAGCATTCTTAACTTTGTTGTGCTGACCGCTT
CGCTGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCAAAA
ATTTTCAGCAAAACCTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTA
TCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTA
TGATCCTGCTGTCGCAAATCGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGT
GGGGTCGCAACCACCATCGGCGGTTTGATTTTCCTGCTCTTTATTATCGGGTTGATTGGTTATCACCCTGATACGCGTAT
CTCGCTGTATGTTGGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAGCGCCGCCACGATCGTCAGCTGG
CTGAAAACCAGTAA

Upstream 100 bases:

>100_bases
TCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTG
TGTTGATGGGTTAGGAATTG

Downstream 100 bases:

>100_bases
TCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCC
CAAATATCCTTCAGATGATG

Product: putative proline-specific permease

Products: Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQ
ENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPK
IFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPG
GVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ

Sequences:

>Translated_457_residues
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQ
ENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPK
IFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPG
GVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ
>Mature_457_residues
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQ
ENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPK
IFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPG
GVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of proline

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family

Homologues:

Organism=Homo sapiens, GI181337167, Length=338, Percent_Identity=24.8520710059172, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI110347453, Length=351, Percent_Identity=24.5014245014245, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI258645169, Length=432, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4507047, Length=364, Percent_Identity=23.6263736263736, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI258614005, Length=430, Percent_Identity=21.8604651162791, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI258614003, Length=430, Percent_Identity=21.8604651162791, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1786602, Length=457, Percent_Identity=100, Blast_Score=912, Evalue=0.0,
Organism=Escherichia coli, GI1786789, Length=452, Percent_Identity=44.9115044247788, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1786302, Length=453, Percent_Identity=43.4878587196468, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI48994972, Length=437, Percent_Identity=45.9954233409611, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1790653, Length=454, Percent_Identity=41.1894273127753, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI87081915, Length=404, Percent_Identity=38.1188118811881, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI87081708, Length=463, Percent_Identity=36.2850971922246, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1788480, Length=406, Percent_Identity=37.192118226601, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789017, Length=431, Percent_Identity=33.6426914153132, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI87081869, Length=235, Percent_Identity=23.4042553191489, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082023, Length=331, Percent_Identity=24.1691842900302, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786694, Length=432, Percent_Identity=23.8425925925926, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=425, Percent_Identity=23.0588235294118, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17531343, Length=360, Percent_Identity=24.4444444444444, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17533459, Length=401, Percent_Identity=22.9426433915212, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540018, Length=465, Percent_Identity=23.4408602150538, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324061, Length=444, Percent_Identity=33.7837837837838, Blast_Score=207, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6320772, Length=404, Percent_Identity=34.6534653465347, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324990, Length=440, Percent_Identity=31.8181818181818, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324924, Length=407, Percent_Identity=31.6953316953317, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322892, Length=475, Percent_Identity=30.3157894736842, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6324059, Length=410, Percent_Identity=33.1707317073171, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319824, Length=432, Percent_Identity=28.0092592592593, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319542, Length=430, Percent_Identity=27.6744186046512, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320717, Length=403, Percent_Identity=28.0397022332506, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321629, Length=479, Percent_Identity=27.1398747390397, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324553, Length=399, Percent_Identity=32.0802005012531, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320251, Length=435, Percent_Identity=27.1264367816092, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319543, Length=399, Percent_Identity=27.8195488721804, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6321053, Length=472, Percent_Identity=26.271186440678, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319608, Length=454, Percent_Identity=24.8898678414097, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6322967, Length=390, Percent_Identity=25.3846153846154, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324981, Length=397, Percent_Identity=25.4408060453401, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320364, Length=511, Percent_Identity=24.853228962818, Blast_Score=109, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24666159, Length=410, Percent_Identity=21.4634146341463, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221512776, Length=410, Percent_Identity=21.4634146341463, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI161077963, Length=336, Percent_Identity=25.8928571428571, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24667468, Length=422, Percent_Identity=22.0379146919431, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROY_ECO57 (P0AAE4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90685
- PIR:   H85535
- RefSeq:   NP_286140.1
- RefSeq:   NP_308479.1
- ProteinModelPortal:   P0AAE4
- EnsemblBacteria:   EBESCT00000024447
- EnsemblBacteria:   EBESCT00000059807
- GeneID:   914554
- GeneID:   957353
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0500
- KEGG:   ecs:ECs0452
- GeneTree:   EBGT00050000009004
- HOGENOM:   HBG492579
- OMA:   LTFQHLG
- ProtClustDB:   PRK10580
- BioCyc:   ECOL83334:ECS0452-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 50216; Mature: 50216

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ed544d0)-; HASH(0x1e7dcda4)-; HASH(0x1e51adf0)-; HASH(0x1c5aa6e0)-; HASH(0x1ee01d38)-; HASH(0x1dab809c)-; HASH(0x1e11c85c)-; HASH(0x1ca2cf58)-; HASH(0x1cdab028)-; HASH(0x1e2ad240)-; HASH(0x1e604538)-; HASH(0x1efd9084)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMR
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
ALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPT
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATIIIMIVAGFGIIIWGIGNGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
PTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHH
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPGGVATTIGGLIFLLFIIGLIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHC
YHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMR
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
ALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVWFPT
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATIIIMIVAGFGIIIWGIGNGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
PTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHH
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPGGVATTIGGLIFLLFIIGLIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHC
YHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796