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The map label for this gene is yafL [H]

Identifier: 218688120

GI number: 218688120

Start: 280504

End: 281262

Strand: Direct

Name: yafL [H]

Synonym: ECED1_0260

Alternate gene names: 218688120

Gene position: 280504-281262 (Clockwise)

Preceding gene: 218688118

Following gene: 218688122

Centisome position: 5.38

GC content: 52.17

Gene sequence:

>759_bases
ATGTCCTTCATGTCTTCGTTCTTGCACGGCAGATTTCTGCATCCAGGCGTGTTTTCGTTATGCGTTTTGCTCCCTTTACT
CGCCAGCGCCACCACATCACATATCTCTTTTAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAAC
AGTACCAAACCCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCAAAAGCAGAAGGGCGCTGCGCCAGCAT
AATCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCGCCGCTCAAGGCGAGTGACAGACGCTGGCAGGCGTTGGT
GGAGAATAATCATTTCTTAAGCAGCGACCATCTACATAACATCACCGAAGTGGCGATTCACCGCCTGGAGCAGCAGCTAG
GTAAGCCTTATATCTGGGGGGGCACGCGGCCTGATAAAGGCTTCGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAG
ATCCTTGAGGCTAAGCTCCCGCGCACGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCT
GCGCCGGGGAGATTTGCTGTTTTTCCATATCCACAGTCGTGAGATAGCCGACCATATGGGCGTATATCTTGGAGACGGTC
AGTTTATCGAGTCACCGCGCACCGGGGAAACCATTCGGGTAAGTCGGTTAGCCGAACCTTTCTGGCAGGACCATTTTTTA
GGCGCGCGACGGATTTTGACGGAAGAGACGATTTTGTAG

Upstream 100 bases:

>100_bases
CTAAGATTATGAAACCGCAATAGTAATATGCAAATAAGCAATTAATAATCCCGTTTACTCCTCCGGTCTTTTAAAATTCT
CACCTTCATTCAGCTGGTTT

Downstream 100 bases:

>100_bases
GCCGGATAAGGCGTTCACGCCGCATCCGGCGGTTGTGCGCCGGTGTCTGATGCGACGCTACGCGTCTTATCATGCCTACA
AATCTGTGCATTTTTTGCGA

Product: putative exported hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSFMSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQASYIVEGNAKSRRALRQH
NREQIKQHPEWFPAPLKASDRRWQALVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNK
ILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFL
GARRILTEETIL

Sequences:

>Translated_252_residues
MSFMSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQASYIVEGNAKSRRALRQH
NREQIKQHPEWFPAPLKASDRRWQALVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNK
ILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFL
GARRILTEETIL
>Mature_251_residues
SFMSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQASYIVEGNAKSRRALRQHN
REQIKQHPEWFPAPLKASDRRWQALVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKI
LEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFLG
ARRILTEETIL

Specific function: Unknown

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=241, Percent_Identity=92.5311203319502, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1787944, Length=134, Percent_Identity=52.2388059701493, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1788501, Length=114, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 29321; Mature: 29190

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFMSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQNRARLLKQYQTHLK
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALVENNHFLSSDHLHN
HHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
ITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRAT
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHE
IVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFL
EEECCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHHHHHHH
GARRILTEETIL
HHHHHHHHHCCC
>Mature Secondary Structure 
SFMSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQNRARLLKQYQTHLK
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALVENNHFLSSDHLHN
HHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
ITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRAT
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHE
IVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFL
EEECCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHHHHHHH
GARRILTEETIL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]