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The map label for this gene is nlpE [H]

Identifier: 218688068

GI number: 218688068

Start: 220043

End: 220753

Strand: Direct

Name: nlpE [H]

Synonym: ECED1_0199

Alternate gene names: 218688068

Gene position: 220043-220753 (Clockwise)

Preceding gene: 218688067

Following gene: 218688076

Centisome position: 4.22

GC content: 52.04

Gene sequence:

>711_bases
ATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGATGTAATAATCGGGCCGAAGT
CGATACGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGTGGCGTGCTGCCGTGTGCTGATT
GCGAAGGAATCGAAACCTCTCTGTTCCTCGAAAAAGACGGAACCTGGGTGATGAATGAACGTTATCTGGGGGCTCGTGAA
GAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGATAGCAAAGGTGAAAA
GTCATATTATCGCGCGAAAGGAGATGCGCTGGAGATGCTCGATCGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACA
CGCTGGAACCGGCACAATCCAGCTTACCCATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCG
ACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTGGCGAATAACGCAGAGCTGGAGCGTGGCTACCTGGCTGCGCG
CGGTAACAGTGAAAAACCAGTGTTACTGTCAGTTGAAGGTCACTTTACGCTGGAGGCTAATCCCGATACCGGTGCGCCGA
CTAAAGTATTAGCGCCCGATACGGCAGGTAAATTTTACCCAAACCAGGATTGCAGTAGTTTGGGGCTGTAA

Upstream 100 bases:

>100_bases
GCATCAAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCG
GGAATAAAAAGAAGGAATGG

Downstream 100 bases:

>100_bases
CCCGTCTTGAGACAGAAACAAATGAAATATTGCCTCATGCGCTGCGCTTATCAGGCCTAAAGCATACAATGCAATATTTT
GAATTAGCACGATTTTGTAG

Product: lipoprotein involved with copper homeostasis and adhesion

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGARE
EPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNQDCSSLGL

Sequences:

>Translated_236_residues
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGARE
EPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNQDCSSLGL
>Mature_236_residues
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGARE
EPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNQDCSSLGL

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=235, Percent_Identity=98.2978723404255, Blast_Score=480, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 25817; Mature: 25817

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00283 SOYBEAN_KUNITZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCCCCCCCCCCEE
LFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEML
EEEECCCCEEECCCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCCHHHHCCCHHHHHH
DREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELE
HCCCCCCCCCCCEEECCCCCCCCCCCHHHCEEEEEEECCCHHHHHCCCCEEEEECCCCCC
RGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNQDCSSLGL
CCEEEECCCCCCCEEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCCCCCCCCCCEE
LFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEML
EEEECCCCEEECCCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCCHHHHCCCHHHHHH
DREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELE
HCCCCCCCCCCCEEECCCCCCCCCCCHHHCEEEEEEECCCHHHHHCCCCEEEEECCCCCC
RGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNQDCSSLGL
CCEEEECCCCCCCEEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]