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The map label for this gene is ribF

Identifier: 218687899

GI number: 218687899

Start: 22646

End: 23587

Strand: Direct

Name: ribF

Synonym: ECED1_0022

Alternate gene names: 218687899

Gene position: 22646-23587 (Clockwise)

Preceding gene: 218687898

Following gene: 218687900

Centisome position: 0.43

GC content: 52.76

Gene sequence:

>942_bases
ATGAAGCTGATACGCGGCATACATAATCTCAGCCAGGCCCCGCAAGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGG
CGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGC
TTTTTGAACCTCAACCACTGGAACTGTTTGCTACTGATAAAGCCCCGGCACGGCTCACCCGGCTGCGGGAAAAACTGCGT
TATCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTTGACAGGCGTTTTGCGGCGTTAACCGCGCAAAACTT
CATCAGTGATCTTCTGGTGAAGCACTTGCGGGTAAAATTTCTTGCCGTAGGTGACGATTTCCGCTTTGGCGCTGGTCGTG
AAGGCGATTTCTTGTTATTACAGAAAGCGGGCATGGAATACGGCTTCGATATCACCAGCACGCAAACTTTTTGCGAAGGT
GGCGTGCGTATCAGCAGCACCGCCGTGCGTCAGGCGCTTGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCA
CCCGTTTGCTATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGC
TACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGTTGGGCCTTGGCGAAAAGCCGTTACCCGGCGTT
GCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAACTGGAAGTGCATTTGTTAGATGTTGCAATGGACCT
TTATGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCATCGCTGGACGAACTGAAAG
CGCAGATTGCGCGTGATGAATTAACCGCCCGAGAATTTTTTGGGCTAACAAAACCGGCTTAA

Upstream 100 bases:

>100_bases
ATGAAAAGCCACTTCTTTCTGATTTCGGTACTCAATCGCCGGTTAACCTTGACCGCTGTACAAGGTATACTCGGACGATT
TTCACTGTTTTGAGCCAGAC

Downstream 100 bases:

>100_bases
GCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAAC
AGGGTTCCCGATGCGTGGCG

Product: bifunctional riboflavin kinase/FMN adenylyltransferase

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGRKRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLR
YLAECGVDYVLCVRFDRRFAALTAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQVSPVKGVYAVEVLGLGEKPLPGV
ANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVLRKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA

Sequences:

>Translated_313_residues
MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGRKRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLR
YLAECGVDYVLCVRFDRRFAALTAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQVSPVKGVYAVEVLGLGEKPLPGV
ANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVLRKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA
>Mature_313_residues
MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGRKRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLR
YLAECGVDYVLCVRFDRRFAALTAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQVSPVKGVYAVEVLGLGEKPLPGV
ANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVLRKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family

Homologues:

Organism=Escherichia coli, GI1786208, Length=313, Percent_Identity=100, Blast_Score=631, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBF_ECO57 (P0AG42)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85483
- PIR:   D90632
- RefSeq:   NP_285719.1
- RefSeq:   NP_308055.1
- ProteinModelPortal:   P0AG42
- SMR:   P0AG42
- MINT:   MINT-1256494
- EnsemblBacteria:   EBESCT00000024961
- EnsemblBacteria:   EBESCT00000056654
- GeneID:   913423
- GeneID:   956690
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0029
- KEGG:   ecs:ECs0028
- GeneTree:   EBGT00050000010293
- HOGENOM:   HBG465096
- OMA:   TFDPHPA
- ProtClustDB:   PRK05627
- BioCyc:   ECOL83334:ECS0028-MONOMER
- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729
- Gene3D:   G3DSA:2.40.30.30
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR22749
- PIRSF:   PIRSF004491
- SMART:   SM00904
- TIGRFAMs:   TIGR00083

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase; SSF82114 Riboflavin_kinase

EC number: =2.7.1.26; =2.7.7.2

Molecular weight: Translated: 34735; Mature: 34735

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGRKRNLPVMVMLFEPQPL
CHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
ELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAALTAQNFISDLLVKHLRVKF
EEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEE
LAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEGGVRISSTAVRQALADDNLAL
EEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCEEHHHHHHHHHCCCCHHH
AESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQVSPVKGVYAVEVLGLGEKPLPGV
HHHHCCCCEEECCEEEECHHHCCCCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCC
ANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVLRKKIRNEQRFASLDELKAQIARDE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTAREFFGLTKPA
HHHHHHCCCCCCC
>Mature Secondary Structure
MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGRKRNLPVMVMLFEPQPL
CHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
ELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAALTAQNFISDLLVKHLRVKF
EEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEE
LAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEGGVRISSTAVRQALADDNLAL
EEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCEEHHHHHHHHHCCCCHHH
AESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQVSPVKGVYAVEVLGLGEKPLPGV
HHHHCCCCEEECCEEEECHHHCCCCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCC
ANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVLRKKIRNEQRFASLDELKAQIARDE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTAREFFGLTKPA
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796