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The map label for this gene is gntU [H]

Identifier: 218550701

GI number: 218550701

Start: 3481300

End: 3482640

Strand: Reverse

Name: gntU [H]

Synonym: EFER_3412

Alternate gene names: 218550701

Gene position: 3482640-3481300 (Counterclockwise)

Preceding gene: 218550702

Following gene: 218550699

Centisome position: 75.9

GC content: 54.96

Gene sequence:

>1341_bases
GTGACTACATTAACGCTTGTTTTAACAGCAGTAGGGTCTGTTTTACTGCTGCTGTTTTTAGTCATGAAGGCGCGTATGCA
CGCTTTCCTGGCTTTGATGGTGGTGTCTATGGGGGCTGGCCTTTTTTCCGGTATGCCGCTCGATAAAATCGCAGCGACAA
TGGAAAAAGGGATGGGAGGCACCCTCGGCTTCCTGGCGGTGGTTGTCGCCCTGGGAGCCATGTTTGGCAAGATCTTACAT
GAAACCGGCGCGGTCGATCAGATTGCCGTCAAAATGCTCAAATCCTTCGGTCACAATCGCGCGCATTATGCCATTGGCCT
TGCGGGCCTGGTTTGTGCGCTACCACTGTTCTTTGAAGTGGCGATTGTCCTGCTGATTAGCGTTGCTTTCTCAATGGCGC
GCCATACCGGCACGAACCTGGTGAAGCTGGTTATCCCGTTATTCGCGGGCGTGGCGGCAGCAGCGGCGTTCTTACTACCG
GGGCCAGCGCCAATGCTGCTGGCATCGCAGATGAATGCTGACTTTGGCTGGATGATCCTGATTGGCCTGTGTGCGGCAAT
CCCGGGAATGATTATTGCCGGGCCGCTGTGGGGTAATTTCATCAGCCGCTACGTGGAGCTGCATATTCCTGACGATATTA
GCGAACCGCATCTCGGCGAAGGCAAAATGCCGTCCTTCGGATTCAGCCTGTCGCTGATCCTGTTGCCACTGGTGCTGGTG
GGGCTGAAAACCATTGCCGCGCGTTTTGTGCCAGAAGGCTCTACCGCTTACGAATGGTTCGAGTTTATTGGTCATCCGTT
TACCGCGATTCTGGTTGCTTGTCTGGTAGCAATTTATGGCCTGGCGATGCGTCGGGGTATGCCAAAAGACAAAGTGATGG
AGATTTGTGGTCACGCGCTGCAACCGGCGGGGATCATTCTGCTGGTGATTGGTGCAGGCGGCGTGTTCAAACAGGTGCTG
GTTGACTCTGGCGTAGGTCCGGCACTGGGCGAAGCGTTAACCGGCATGGGCCTGCCGATTGCCATCACCTGCTTTGTGCT
GGCTGCGGCGGTGCGTATCATTCAGGGTTCTGCCACCGTTGCCTGTTTAACGGCGGTGGGACTGGTGATGCCGGTTATTG
AACAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCCGGTGGTTCGATTGTTGTCAGCCACGTT
AACGACGCTGGTTTCTGGTTGTTCGGTAAATTTACCGGCGCGACCGAAGCCGAAACGCTGAAAACCTGGACCATGATGGA
AACCATCCTCGGCACTGTTGGTGCCATCGTCGGGATGATTGCATTCCAGCTGTTGAGTTAA

Upstream 100 bases:

>100_bases
GGGTGCGGACGAAACCGATGTACTGGTGGTGGATATCGATCAACCGCTGGAAGGTGTTGTGGCAAGCACCATTGAGGTTA
TTAAAAAAGGCAAATAAGTA

Downstream 100 bases:

>100_bases
GTTTGTTCGCCCGGTAGTTGTGACGCTACCGGGCTCTTTTCGAAAAACTCTCCTCGTTACCCCTTCATCCACATTCGAAT
GCCGTCAAGGAACATCTGGG

Product: low affinity gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease; Gnt-I system [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILH
ETGAVDQIAVKMLKSFGHNRAHYAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLLP
GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLV
GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRRGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVL
VDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHV
NDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS

Sequences:

>Translated_446_residues
MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILH
ETGAVDQIAVKMLKSFGHNRAHYAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLLP
GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLV
GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRRGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVL
VDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHV
NDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS
>Mature_445_residues
TTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHE
TGAVDQIAVKMLKSFGHNRAHYAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLLPG
PAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLVG
LKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRRGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLV
DSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVN
DAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS

Specific function: Part of the gluconate utilization system Gnt-I; low- affinity intake of gluconate [H]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994939, Length=446, Percent_Identity=99.3273542600897, Blast_Score=869, Evalue=0.0,
Organism=Escherichia coli, GI1790716, Length=430, Percent_Identity=37.2093023255814, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI48994935, Length=434, Percent_Identity=36.6359447004608, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI2367370, Length=429, Percent_Identity=34.032634032634, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1789097, Length=450, Percent_Identity=32.8888888888889, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788707, Length=429, Percent_Identity=35.1981351981352, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1790776, Length=431, Percent_Identity=31.0904872389791, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 46486; Mature: 46355

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCC
TLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHNRAHYAIGLAGLVCALPLFFEV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLLPGPAPMLLASQMNADFGWMIL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHH
IGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLV
HHHHHHCCHHHEECHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRRGMPKDKVMEICGHAL
HHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
QPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATV
CCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHH
ACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVNDAGFWLFGKFTGATEAETL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCHHHHH
KTWTMMETILGTVGAIVGMIAFQLLS
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]