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The map label for this gene is prpR [H]

Identifier: 218549976

GI number: 218549976

Start: 2737467

End: 2739053

Strand: Direct

Name: prpR [H]

Synonym: EFER_2661

Alternate gene names: 218549976

Gene position: 2737467-2739053 (Clockwise)

Preceding gene: 218549971

Following gene: 218549978

Centisome position: 59.66

GC content: 55.45

Gene sequence:

>1587_bases
ATGGCACATCCACCACGGCTGAATGACGACAAACCGGTTATCTGGACGGTATCTGTAACGCGCCTGTTCGAGCTGTTTCG
CGATATCAGTCTCGAGTTTGATCACCTGGCAAACATTACCCCTATCCAGCTTGGCTTTGAAAAAGCGGTGGCCTACATCC
GCAAAAAACTGGCCAGCGAACGCTGCGACGCCATCATTGCCGCTGGCTCTAACGGCGCGTACCTGAAAAGCCGCCTGTCG
GTACCGGTAATTCTGATAAAACCGAGCGGCTACGATGTGTTACAGGCACTGGCAAAAGCCGGAAAACTCACCTCTTCTAT
CGGCGTTGTCACCTATCAGGAAACCATTCCGGCATTGGTAGCGTTTCAAAAAACCTTTAGTTTGCGCCTCGACCAACGTA
GCTACATCACCGAAGAAGACGCCCGCGGGCAAATCAATGAGTTGAAAGCCAACGGCACCGAAGCGGTGGTCGGCGCAGGG
CTGATTACCGATCTGGCGGAAGAAGCCGGAATGACCGGAATTTTTATCTATTCTGCCGCCACCGTACGCCAGGCGTTCAG
CGATGCGCTGGATATGACGCGCATGTCTCTACGGCATAACGCGCACGATGCTACCCGCAATGCCCTGCGTACTCGTTACG
TGCTGGGCGATATGCTCGGTCAATCACCACAGATGGAACAGGTGCGGCAGACTATTTTGCTGTATGCCCGTTCCAGTGCG
GCGGTGTTGATTGAAGGGGAAACGGGGACGGGCAAAGAACTGGCGGCCCAGGCGATTCATCGGGAATACTTTGCCCGCCA
CGATGCACGACGGGGCAAAAAGTCACATCCGTTTGTTGCCGTCAACTGCGGGGCGATTGCCGAATCGCTGCTGGAGGCGG
AACTGTTTGGCTATGAGGAAGGGGCGTTTACCGGCTCGCGACGCGGAGGACGCGCCGGGCTGTTTGAAATCGCCCACGGT
GGCACGCTGTTTCTTGACGAGATAGGCGAAATGCCGCTGCCGTTGCAGACCCGGCTGCTGCGGGTGCTGGAAGAAAAAGA
GGTCACCCGCGTCGGCGGGCATCAGCCTGTTCCGGTGGATGTGCGGGTTATTAGCGCCACTCACTGCAATCTCGAAGAGG
ATATGCAGCAAGGGCGTTTTCGCCGTGATCTGTTTTATCGACTGAGTATTTTGCGTCTGCAATTGCCGCCGCTACGCGAG
CGGGTGGCGGATATTCTGCCGCTGGCGGAAAGCTTTTTGAAAGTGTCACTGGCGGCACTTACTGCGCCGTTTTCTTCCGC
GTTACGTCAGGGATTACAGGCAAGTGAAACTGTGCTGTTGCGCTACAACTGGCCGGGAAATATTCGTGAACTGCGCAATA
TGATGGAACGACTGGCGCTATTTTTAAGTGTGGAACCGACGCCGGATTTAACGCCGCAATTTTTGCAGTTGCTACTGCCG
GAACTGGCGCGCGAGTCGGCGGAAACTCCCGGTCCATGCTTACTGACGCCACAACAGGCACTGGAGAAATTTAATGGCGA
TAAAACAGCCGCGGCGAATTATTTAGGTATCAGCCGAACGACATTCTGGCGGCGGCTGAAAAACTGA

Upstream 100 bases:

>100_bases
TTTCAGTTAACGTTTCAGGCAATGTTTCACTGCATTTCATTGCAACAATTATGAAACAAGACTAAACCCAATATTCGGTT
TCTTAACTTTGCGGTGCGCT

Downstream 100 bases:

>100_bases
AATGCCGAATCCAGCGATAACGCTTTATCCGGCATAAATTTCAATAGATGATCACCGATAAACTTACGCATCGGGCATGT
TTACATGGGGTGCAGAATTA

Product: DNA-binding transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASERCDAIIAAGSNGAYLKSRLS
VPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFSLRLDQRSYITEEDARGQINELKANGTEAVVGAG
LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNAHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
AVLIEGETGTGKELAAQAIHREYFARHDARRGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHG
GTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGRFRRDLFYRLSILRLQLPPLRE
RVADILPLAESFLKVSLAALTAPFSSALRQGLQASETVLLRYNWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
ELARESAETPGPCLLTPQQALEKFNGDKTAAANYLGISRTTFWRRLKN

Sequences:

>Translated_528_residues
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASERCDAIIAAGSNGAYLKSRLS
VPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFSLRLDQRSYITEEDARGQINELKANGTEAVVGAG
LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNAHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
AVLIEGETGTGKELAAQAIHREYFARHDARRGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHG
GTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGRFRRDLFYRLSILRLQLPPLRE
RVADILPLAESFLKVSLAALTAPFSSALRQGLQASETVLLRYNWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
ELARESAETPGPCLLTPQQALEKFNGDKTAAANYLGISRTTFWRRLKN
>Mature_527_residues
AHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASERCDAIIAAGSNGAYLKSRLSV
PVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFSLRLDQRSYITEEDARGQINELKANGTEAVVGAGL
ITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNAHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSAA
VLIEGETGTGKELAAQAIHREYFARHDARRGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGG
TLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGRFRRDLFYRLSILRLQLPPLRER
VADILPLAESFLKVSLAALTAPFSSALRQGLQASETVLLRYNWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLPE
LARESAETPGPCLLTPQQALEKFNGDKTAAANYLGISRTTFWRRLKN

Specific function: Involved in the transcriptional regulation of the propionate catabolism operon [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1786524, Length=528, Percent_Identity=97.3484848484848, Blast_Score=1051, Evalue=0.0,
Organism=Escherichia coli, GI1790299, Length=345, Percent_Identity=43.4782608695652, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788550, Length=338, Percent_Identity=43.7869822485207, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1790437, Length=327, Percent_Identity=42.2018348623853, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082117, Length=355, Percent_Identity=42.8169014084507, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=38.855421686747, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1788905, Length=381, Percent_Identity=37.7952755905512, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789087, Length=332, Percent_Identity=38.855421686747, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI87082152, Length=246, Percent_Identity=45.5284552845528, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87081872, Length=241, Percent_Identity=43.9834024896266, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787583, Length=237, Percent_Identity=45.1476793248945, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI87081858, Length=307, Percent_Identity=32.2475570032573, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=35.5648535564854, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR012704
- InterPro:   IPR010524 [H]

Pfam domain/function: PF02954 HTH_8; PF06506 PrpR_N; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58555; Mature: 58424

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASE
CCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALV
HCCEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCHHHCCEEEEHHHHHHHH
AFQKTFSLRLDQRSYITEEDARGQINELKANGTEAVVGAGLITDLAEEAGMTGIFIYSAA
HHHHHHHHEECCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHH
TVRQAFSDALDMTRMSLRHNAHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
AVLIEGETGTGKELAAQAIHREYFARHDARRGKKSHPFVAVNCGAIAESLLEAELFGYEE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVD
CCCCCCCCCCCCCEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VRVISATHCNLEEDMQQGRFRRDLFYRLSILRLQLPPLRERVADILPLAESFLKVSLAAL
EEEEEEECCCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAPFSSALRQGLQASETVLLRYNWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
ELARESAETPGPCLLTPQQALEKFNGDKTAAANYLGISRTTFWRRLKN
HHHHHCCCCCCCEEECHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
AHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVAYIRKKLASE
CCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALV
HCCEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCHHHCCEEEEHHHHHHHH
AFQKTFSLRLDQRSYITEEDARGQINELKANGTEAVVGAGLITDLAEEAGMTGIFIYSAA
HHHHHHHHEECCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHH
TVRQAFSDALDMTRMSLRHNAHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
AVLIEGETGTGKELAAQAIHREYFARHDARRGKKSHPFVAVNCGAIAESLLEAELFGYEE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVD
CCCCCCCCCCCCCEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VRVISATHCNLEEDMQQGRFRRDLFYRLSILRLQLPPLRERVADILPLAESFLKVSLAAL
EEEEEEECCCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAPFSSALRQGLQASETVLLRYNWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
ELARESAETPGPCLLTPQQALEKFNGDKTAAANYLGISRTTFWRRLKN
HHHHHCCCCCCCEEECHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]