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The map label for this gene is pcaK [H]

Identifier: 218549912

GI number: 218549912

Start: 2667093

End: 2668568

Strand: Direct

Name: pcaK [H]

Synonym: EFER_2597

Alternate gene names: 218549912

Gene position: 2667093-2668568 (Clockwise)

Preceding gene: 218549911

Following gene: 218549913

Centisome position: 58.12

GC content: 49.46

Gene sequence:

>1476_bases
TTGAAGTTCCGCTCAGCGGGAAACAGGGGGCATTACCCGCCGAAAAGCGCGCCCAATTACTGTTTAACGCAGCACAGGCT
TACCTGTATCCGTCGGTATAAGGGGAAGATGATGGCACAATCAGATTTGCTCGACGTGCGAGAATTAATCAATCGCAATC
CTCTTAGCCGCTACCAAAAGCTGGTGATCTTATTAGGCTTTGTTGTGATCGCACTGGATGGCTTTGATATTGCGATTATG
GGATTTATCGCCCCGGTACTTAAGCAGGAATGGGGGGCATCTAATCATGAACTTGGATTTGTCATTAGCGCCGCTCTGAT
TGGGCTGGCATTGGGTGCCATGTTTTCCGGACCGCTCGCCGACTGGCTGGGACGCAAAAAAATCATCATTAATAGCGTGT
TCTTTTTTGGTATCTGGACCGTAGCAACCGCCTTTTCACAAAATATAGAGCAGATGATTTTCTTTCGTTTTATGACCGGA
TTGGGGCTTGGCGCTGCAATGCCTAATATGAGTACGCTGGTCTCAGAATATGCCCCTGAACGTAAGCGCTCTTTTATTAT
TACGATTATTTTCTGCGGTTTTACTTTTGGTGCAGCAGCTGGTGGTTTTGCCGCTTCATGGCTAATCCCACAATTTGGCT
GGCATTCGCTGATGGCTCTTGGCGGTATTTTACCCGTCCTGTTTGCTCCGGTTCTGATTTGGCTCCTGCCGGAATCAGTA
AGATTTCTGGTTATCAAACGCGCGCCACCTGTTCGCATTCGCATGATCTTACAGCGTCTATACCCGGGGAAAATTGCCGA
TAACGTCGAGTTTATTTTGCCTACACAACCGCTGGATAAAAGTGCCATGCGGATTGTGCTTTCACCGCAATATCGCTTTG
GCTCCCTGATGCTTTGGCTGGTCTATTTTATGGGACTGTTTCTGGTCTATATACTTGGTAGCTGGTTACCAACGCTGGTA
AAAGAGGTGGGAATGACTGTAAGTCAGGCTGCAGTGATAACAGCCCTTTATCAGGCAGGCGGTACGATAGGTTCGCTGTT
TGCCGGTTGGCTTATGGATAAGATTAATCCCCATCGGGCGCTGGGTGTTATTTACGGCGTTGGGGGGCTTATCACCATGT
CGATGGGCTATTTTTCCACCAGCTTTACACTCCTTTGTCTGCTTGCCTTCATCTGCGGGGCTTGTCTGAATGGTGCCAAC
ACCGGGATGAACGCAATGTCAGCACGTTTTTATCCTACACAAGCGCGGGCGACAGGCTCCAGTTGGATGCATGGTATCGG
GCGAATTGGTGCCATCCTCAGCGCCTTTGCCGGGGCGGAGATGATGGCAATGGAGTTGAGTTTCGAAGCGGTGTTCCTGC
TTTTGGGGATCCCGGCTGCAATTACCGTAGTGGCGATATCAGCAAAAGGTATGTTTGCTTCATCGAGTACTCACGAGTCA
ACACCTGTCGCTTCAGCGCTTAGAGAACCTTCCTGA

Upstream 100 bases:

>100_bases
ATTATTCGTCAGGCGCGTACAGCACGCCAGATCCCAGGTGAAGGAGGGCTGAATTTGCACGGCCTGATGGCCGCATTACC
AGCCGATCTCCCGGTATCCG

Downstream 100 bases:

>100_bases
AATACTCCATACAGCAAATCTTGAGCGCTAAAGTCAGATTACTGGCTTTAGCGCACAAATCATTAATCGAATTAATATTT
GTTAATTATCGCTCTGTTTA

Product: 4-hydroxybenzoate transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKFRSAGNRGHYPPKSAPNYCLTQHRLTCIRRYKGKMMAQSDLLDVRELINRNPLSRYQKLVILLGFVVIALDGFDIAIM
GFIAPVLKQEWGASNHELGFVISAALIGLALGAMFSGPLADWLGRKKIIINSVFFFGIWTVATAFSQNIEQMIFFRFMTG
LGLGAAMPNMSTLVSEYAPERKRSFIITIIFCGFTFGAAAGGFAASWLIPQFGWHSLMALGGILPVLFAPVLIWLLPESV
RFLVIKRAPPVRIRMILQRLYPGKIADNVEFILPTQPLDKSAMRIVLSPQYRFGSLMLWLVYFMGLFLVYILGSWLPTLV
KEVGMTVSQAAVITALYQAGGTIGSLFAGWLMDKINPHRALGVIYGVGGLITMSMGYFSTSFTLLCLLAFICGACLNGAN
TGMNAMSARFYPTQARATGSSWMHGIGRIGAILSAFAGAEMMAMELSFEAVFLLLGIPAAITVVAISAKGMFASSSTHES
TPVASALREPS

Sequences:

>Translated_491_residues
MKFRSAGNRGHYPPKSAPNYCLTQHRLTCIRRYKGKMMAQSDLLDVRELINRNPLSRYQKLVILLGFVVIALDGFDIAIM
GFIAPVLKQEWGASNHELGFVISAALIGLALGAMFSGPLADWLGRKKIIINSVFFFGIWTVATAFSQNIEQMIFFRFMTG
LGLGAAMPNMSTLVSEYAPERKRSFIITIIFCGFTFGAAAGGFAASWLIPQFGWHSLMALGGILPVLFAPVLIWLLPESV
RFLVIKRAPPVRIRMILQRLYPGKIADNVEFILPTQPLDKSAMRIVLSPQYRFGSLMLWLVYFMGLFLVYILGSWLPTLV
KEVGMTVSQAAVITALYQAGGTIGSLFAGWLMDKINPHRALGVIYGVGGLITMSMGYFSTSFTLLCLLAFICGACLNGAN
TGMNAMSARFYPTQARATGSSWMHGIGRIGAILSAFAGAEMMAMELSFEAVFLLLGIPAAITVVAISAKGMFASSSTHES
TPVASALREPS
>Mature_491_residues
MKFRSAGNRGHYPPKSAPNYCLTQHRLTCIRRYKGKMMAQSDLLDVRELINRNPLSRYQKLVILLGFVVIALDGFDIAIM
GFIAPVLKQEWGASNHELGFVISAALIGLALGAMFSGPLADWLGRKKIIINSVFFFGIWTVATAFSQNIEQMIFFRFMTG
LGLGAAMPNMSTLVSEYAPERKRSFIITIIFCGFTFGAAAGGFAASWLIPQFGWHSLMALGGILPVLFAPVLIWLLPESV
RFLVIKRAPPVRIRMILQRLYPGKIADNVEFILPTQPLDKSAMRIVLSPQYRFGSLMLWLVYFMGLFLVYILGSWLPTLV
KEVGMTVSQAAVITALYQAGGTIGSLFAGWLMDKINPHRALGVIYGVGGLITMSMGYFSTSFTLLCLLAFICGACLNGAN
TGMNAMSARFYPTQARATGSSWMHGIGRIGAILSAFAGAEMMAMELSFEAVFLLLGIPAAITVVAISAKGMFASSSTHES
TPVASALREPS

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=473, Percent_Identity=23.0443974630021, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI7662270, Length=308, Percent_Identity=23.0519480519481, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI166064021, Length=361, Percent_Identity=24.6537396121884, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI154350196, Length=290, Percent_Identity=26.551724137931, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI87081723, Length=390, Percent_Identity=33.5897435897436, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI87082404, Length=446, Percent_Identity=24.4394618834081, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87082231, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786229, Length=378, Percent_Identity=23.8095238095238, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788074, Length=403, Percent_Identity=27.7915632754342, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082159, Length=391, Percent_Identity=22.5063938618926, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789207, Length=358, Percent_Identity=24.3016759776536, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87082320, Length=309, Percent_Identity=20.7119741100324, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=456, Percent_Identity=25.219298245614, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24640198, Length=458, Percent_Identity=22.9257641921397, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24640196, Length=458, Percent_Identity=22.9257641921397, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24640200, Length=458, Percent_Identity=22.9257641921397, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19922874, Length=447, Percent_Identity=22.1476510067114, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53530; Mature: 53530

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFRSAGNRGHYPPKSAPNYCLTQHRLTCIRRYKGKMMAQSDLLDVRELINRNPLSRYQK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LVILLGFVVIALDGFDIAIMGFIAPVLKQEWGASNHELGFVISAALIGLALGAMFSGPLA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHH
DWLGRKKIIINSVFFFGIWTVATAFSQNIEQMIFFRFMTGLGLGAAMPNMSTLVSEYAPE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCH
RKRSFIITIIFCGFTFGAAAGGFAASWLIPQFGWHSLMALGGILPVLFAPVLIWLLPESV
HHHHEEEEHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
RFLVIKRAPPVRIRMILQRLYPGKIADNVEFILPTQPLDKSAMRIVLSPQYRFGSLMLWL
EEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHEEEEECCCHHHHHHHHHH
VYFMGLFLVYILGSWLPTLVKEVGMTVSQAAVITALYQAGGTIGSLFAGWLMDKINPHRA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
LGVIYGVGGLITMSMGYFSTSFTLLCLLAFICGACLNGANTGMNAMSARFYPTQARATGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHCCCCH
SWMHGIGRIGAILSAFAGAEMMAMELSFEAVFLLLGIPAAITVVAISAKGMFASSSTHES
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCC
TPVASALREPS
CHHHHHHCCCC
>Mature Secondary Structure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CHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]