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The map label for this gene is priC [H]

Identifier: 218549865

GI number: 218549865

Start: 2613839

End: 2614366

Strand: Direct

Name: priC [H]

Synonym: EFER_2550

Alternate gene names: 218549865

Gene position: 2613839-2614366 (Clockwise)

Preceding gene: 218549858

Following gene: 218549866

Centisome position: 56.96

GC content: 59.28

Gene sequence:

>528_bases
GTGAAAACCGCCCTGCTGCTGGAAAAACTGGAAGGTCAGCTCGCCACGCTGCGCCAGCGTTGTGCCCCGGTGGCGCAGTT
CGCCACGCTAAGCGCCCGTTTCGACAGGCGCCTTTTTCAAACCCGTGCGACAACTCTACAGGCGTGCCTGGAGGAAGCGG
GAGATAATTTGTCCGCGCTTCGCCATGCAGTTGAGCAACAGCAACTGCCGCAAGTGGCCTGGCTGGCGGAACATCTGGCA
GCACAACTGGAAGCCATAGCGCGTGAAGCCGCCGCCTGGTCATTGCGCGAGTGGGACAGTGCGCCACCGAAAATTGCCCG
CTGGCAGCGTAAACGTATTCAGCATCAGGATTTTGAGCGGCGACTTCGTGAGATGGTTGCCGAACGTAGAGCCCGTCTGG
CGCAAGCGGCCGATCTCGTGGAACAACAAACGCTGCATCGTGAAGTGGAAGCCTATGAAGCGCGCCTTGCGCGGTGTCGC
CATGCGCTGGAAAAAATCGAAAACAGGTTAGCGCGTTTAACCCGCTAA

Upstream 100 bases:

>100_bases
AGCCAATGATGATTAAAATGATTCGTTGCATATTATGTGTAGTCAGCTTACAGAGTGGCTACTTTAGCATAACAATTATC
ATTTTCATTGAGGTCTTATC

Downstream 100 bases:

>100_bases
CAATGGAGAGAATATGTCACTGGAAAATGCCCCTGACGATGTCAAACTGGCCGTCGATTTAATTGTCCTGCTGGAAGAAA
ATCAGATCCCTGCCAGTACC

Product: primosomal replication protein N''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRRLFQTRATTLQACLEEAGDNLSALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREAAAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLAQAADLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR

Sequences:

>Translated_175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRRLFQTRATTLQACLEEAGDNLSALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREAAAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLAQAADLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR
>Mature_175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRRLFQTRATTLQACLEEAGDNLSALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREAAAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLAQAADLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR

Specific function: PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase [H]

COG id: COG3923

COG function: function code L; Primosomal replication protein N''

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786673, Length=175, Percent_Identity=95.4285714285714, Blast_Score=327, Evalue=3e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010890 [H]

Pfam domain/function: PF07445 priB_priC [H]

EC number: NA

Molecular weight: Translated: 20307; Mature: 20307

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRRLFQTRATTLQACLEEAGDNLSAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAPPKIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLREMVAERRARLAQAADLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRRLFQTRATTLQACLEEAGDNLSAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAPPKIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLREMVAERRARLAQAADLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1856227; 9278503 [H]