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The map label for this gene is rbsB [H]

Identifier: 218549835

GI number: 218549835

Start: 2584866

End: 2585801

Strand: Direct

Name: rbsB [H]

Synonym: EFER_2518

Alternate gene names: 218549835

Gene position: 2584866-2585801 (Clockwise)

Preceding gene: 218549834

Following gene: 218549836

Centisome position: 56.33

GC content: 43.59

Gene sequence:

>936_bases
ATGAAATTACGTTTAACACTGTTAACCGCTGCGACCCTGACTGCTCTTTCTTTTTCTGCCCATGCGGTAGAAAAAGGAAC
CATTATGATTATGGTCAATTCACTGGATAATCCTTATTACGCCTCGGAAGCGAAAGGTGCCAGTGAAAAAGCCCAGCAAC
TGGGATATAAAACGACCATTCTTTCTCATGGTGAAGATGTCAAAAAACAGAATGAACTGATCGATACTGCAATTGGTAAA
AAAGTTCAGGGTATTATTTTAGATAATGCTGACTCTACTGCCAGTGTTGCAGCCATTGAAAAAGCAAAAAAAGCAGGTAT
TCCCGTTATATTAATTAATCGCGAAATACCTGTGGATGATGTTGCACTGGTGCAAATTACCCATAATAACTTCCAGGCTG
GCTCGGAAGTTGCCAACGTATTTGTCGAGAAAATGGGAGAGAAAGGAAAATATGCCGAACTCACCTGTAATCTTGCTGAT
AATAATTGCGTAACTCGCTCGAAATCTTTCCACCAGGTCATTGACCAGTTCCCTGAAATGGTCAGTGTGGCGAAGCAAGA
TGCCAAAGGCACGTTAATTGATGGCAAACGAATAATGGACAGTATTCTGCAAGCCCATCCCGATGTTAAAGGGGTGATTT
GTGGTAATGGTCCGGTTGCACTGGGAGCTATCGCAGCATTGAAAGCAGCCAATCGCAATGACGTTATTGTTGTCGGCATT
GATGGCAGTAACGATGAACGCGATGCCGTGAAAGCCGAATCATTGCAGGCTACCGTGATGTTACAAGCACAAGCCATTGC
CGCGCAGGGTGTTACCGATCTGGATAATTACCTGCAAAAAGGAGTTAAACCAGCCAAACAGCGGGTGATGTTCCGCGGCA
TTCTGATAACCAAAGATAACGCAGATAAAGTCCAGGATTTCAATATCAAATCCTGA

Upstream 100 bases:

>100_bases
ACAAGACTAACCAACGGAAGGAGAATACCGGCCCATAAATAATTCAGGCTTTACGTTGTTTCACCGTACCAGACAATAAC
AACACAGAGAGAACATTGTC

Downstream 100 bases:

>100_bases
TCCCAGGTCGTTGCGCCCTCACAATGAGGGCGCAGAGGAGAATATTATGGATAAAAAAGTCTGGCTGGCGCTGGCAATTC
TGAGCCTGGGTGGATGTCGC

Product: ABC transporter sugar-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEAKGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGK
KVQGIILDNADSTASVAAIEKAKKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANVFVEKMGEKGKYAELTCNLAD
NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGPVALGAIAALKAANRNDVIVVGI
DGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDNYLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS

Sequences:

>Translated_311_residues
MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEAKGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGK
KVQGIILDNADSTASVAAIEKAKKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANVFVEKMGEKGKYAELTCNLAD
NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGPVALGAIAALKAANRNDVIVVGI
DGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDNYLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS
>Mature_311_residues
MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEAKGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGK
KVQGIILDNADSTASVAAIEKAKKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANVFVEKMGEKGKYAELTCNLAD
NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGPVALGAIAALKAANRNDVIVVGI
DGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDNYLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=281, Percent_Identity=34.1637010676157, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790526, Length=272, Percent_Identity=25.3676470588235, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788898, Length=250, Percent_Identity=26.8, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790674, Length=257, Percent_Identity=25.2918287937743, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33301; Mature: 33301

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEAKGASEKAQQLGYKTTI
CEEEEEEHHHHHHHHHHHHHHEECCCEEEEEEECCCCCEECCCCCCCHHHHHHCCCCEEE
LSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKAKKAGIPVILINREIPVDD
ECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
VALVQITHNNFQAGSEVANVFVEKMGEKGKYAELTCNLADNNCVTRSKSFHQVIDQFPEM
EEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGPVALGAIAALKAANRNDVIVVGI
HHHHHHCCCCEEECCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEE
DGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDNYLQKGVKPAKQRVMFRGILITKDN
CCCCCCHHHHHHHHHEEEEEEEEHHHHHCCCHHHHHHHHHCCCHHHHHHHHEEEEEECCC
ADKVQDFNIKS
CCCCCCCCCCC
>Mature Secondary Structure
MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEAKGASEKAQQLGYKTTI
CEEEEEEHHHHHHHHHHHHHHEECCCEEEEEEECCCCCEECCCCCCCHHHHHHCCCCEEE
LSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKAKKAGIPVILINREIPVDD
ECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
VALVQITHNNFQAGSEVANVFVEKMGEKGKYAELTCNLADNNCVTRSKSFHQVIDQFPEM
EEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGPVALGAIAALKAANRNDVIVVGI
HHHHHHCCCCEEECCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEE
DGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDNYLQKGVKPAKQRVMFRGILITKDN
CCCCCCHHHHHHHHHEEEEEEEEHHHHHCCCHHHHHHHHHCCCHHHHHHHHEEEEEECCC
ADKVQDFNIKS
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]