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The map label for this gene is miaB [H]

Identifier: 218549762

GI number: 218549762

Start: 2505106

End: 2506530

Strand: Direct

Name: miaB [H]

Synonym: EFER_2441

Alternate gene names: 218549762

Gene position: 2505106-2506530 (Clockwise)

Preceding gene: 218549760

Following gene: 218549763

Centisome position: 54.59

GC content: 53.47

Gene sequence:

>1425_bases
ATGACCAAAAAACTCCATATTAAAACCTGGGGCTGTCAGATGAACGAGTACGATTCATCGAAGATGGCCGATCTGCTGGA
TGCCACCCACGGCTATCAACTGACCGACGTGGCGGAAGAAGCGGATGTGCTGCTGCTGAACACCTGCTCAATCCGCGAGA
AGGCTCAGGAAAAAGTCTTCCATCAGTTGGGTCGCTGGAAACTGTTAAAAGAGAAGAATCCAGACCTGATTATCGGCGTC
GGCGGCTGCGTGGCATCGCAAGAAGGCGAGCACATTCGCCAGCGCGCCCACTATGTTGATATTATTTTTGGGCCGCAAAC
GCTGCACCGTCTGCCGGAGATGATCAACTCCGTACGCGGCGACCGCAGCCCGGTTGTAGATATCAGCTTTCCGGAAATCG
AGAAGTTTGACCGTCTGCCGGAACCGCGTGCCGAAGGACCAACCGCGTTTGTCTCCATCATGGAAGGCTGCAATAAATAT
TGCACCTACTGTGTGGTGCCTTACACCCGTGGTGAAGAGGTAAGCCGTCCTTCCGACGATATTCTGTTTGAGATTGCCCA
GCTTGCGGCTCAGGGCGTGCGTGAAGTCAACCTGCTCGGTCAGAACGTGAACGCCTGGCGTGGTGAGAACTACGACGGCA
CCACCGGATCGTTTGCCGATCTGCTGCGTCTGGTTGCGGCGATCGACGGGATCGATCGTATTCGCTTTACCACCAGCCAT
CCGATCGAATTCACCGACGATATCATCGAAGTGTACCGCGACACGCCGGAGCTGGTGAGCTTCCTGCATCTGCCGGTTCA
GAGCGGTTCCGATCGCATTCTAAACCTGATGGGACGTACCCACACGGCGCTGGAGTACAAAGCGATCATCCGTAAATTGC
GTGCGGCGCGTCCTGATATTCAGATCAGTTCTGACTTCATCGTTGGCTTCCCTGGCGAAACCACTGAAGACTTCGAAAAA
ACGATGAAGCTGATTGCCGACGTCAATTTCGACATGAGCTATAGCTTTATCTTCTCCGCTCGTCCAGGTACGCCAGCCGC
TGATATGGTTGATGATGTTCCGGAAGAAGAGAAGAAGCAGCGTCTGTATATTCTGCAAGAGCGCATTAATCAGCAAGCGA
TGGCATGGAGCCGCCGTATGCTCGGCACCACCCAGCGTATTCTGGTAGAAGGTACATCGCGTAAGAGCATCATGGAGCTT
TCCGGTCGTACTGAAAATAACCGCGTGGTTAACTTCGAAGGCACGCCGGATATGATCGGTAAATTCGTCGATGTAGAAAT
TACCGACGTCTACCCGAACTCTCTGCGCGGTAAAGTGGTGCGTACTGAAGATGAAATGGGTCTGCGCGTGGCAGAAACAC
CGGAATCGGTGATTGCCCGTACCCGCAAAGAAAATGACCTGGGCGTGGGTTATTATCAGCCGTAA

Upstream 100 bases:

>100_bases
TACCCCCAAGGGCTGATAACGCTCATACTGGTCACAACGGCAGCAAAGCATTACACTATGCGCCCTGCATTCCTGGCTAC
TATTTCGCAAGAGCAAGTCG

Downstream 100 bases:

>100_bases
TTCTCAGGCCCACCGTTCCGGTGGGCCTTGTTTTTTCTCTCTTTATCCCCATCTTTATCGCAATGCTTGCGCCTTTTTCC
TGTGGCGTGAATAATTTCCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGV
GGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEK
TMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMEL
SGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP

Sequences:

>Translated_474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGV
GGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEK
TMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMEL
SGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
>Mature_473_residues
TKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVG
GCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
TYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP
IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKT
MKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELS
GRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=37.4220374220374, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=37.4476987447699, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=30.4824561403509, Blast_Score=180, Evalue=3e-45,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=100, Blast_Score=984, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=385, Percent_Identity=28.3116883116883, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=33.1168831168831, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=345, Percent_Identity=28.1159420289855, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=37.1824480369515, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI19922432, Length=493, Percent_Identity=28.6004056795132, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53664; Mature: 53532

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEHHHHCCCCEEEEECCHHHHHHHHHHH
HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
HHHCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCC
DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEECCC
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHH
RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHC
KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
CEEEEEEEECCCCCCCCCEEEEHHHCCCEEECCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEHHHHCCCCEEEEECCHHHHHHHHHHH
HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
HHHCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCC
DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEECCC
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHH
RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHC
KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
CEEEEEEEECCCCCCCCCEEEEHHHCCCEEECCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA