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The map label for this gene is nac [H]

Identifier: 218549293

GI number: 218549293

Start: 2003216

End: 2004133

Strand: Reverse

Name: nac [H]

Synonym: EFER_1955

Alternate gene names: 218549293

Gene position: 2004133-2003216 (Counterclockwise)

Preceding gene: 218549295

Following gene: 218549292

Centisome position: 43.68

GC content: 48.58

Gene sequence:

>918_bases
ATGAATTTCAGACGCCTGAAATACTTCGTCAAAATCGTCGATATCGGTAGCCTGACTCAGGCTGCAGAAGTCCTGCATAT
TGCCCAGCCTGCGCTCAGCCAGCAGGTTGCGACGCTTGAAGGTGAGTTGGATCAGCAACTGCTCATCCGTACTAAACGCG
GAGTAACGCCAACGGAAGCCGGGAAAATTCTCTATGCTCACGCCAGAACGATATTACGCCAGTGCGAACAAGCGCAACTG
GCAGTACATAATGCCTGTCAGAGTTTAAGCGGTCAGGTGTCAATTGGCCTTGCGCCTGGTACAGCGGCATCTTCAATGAC
AATGCCATTGTTACAGGCTGTACGTACTGAACTGCCCGATGTGTTGGTTTATTTACATGAAAATAGTGGTGCGGCACTTA
ATGATAAGTTGCTCAATGGCGAGCTGGATATGGCTGTAATGTACGAACGCTCTCCCATTGACGGCATTCATTGCCAGCCG
TTACTTAAAGAAGATCTTTATTTAGTCGGTACACAAGATTGCCCTGGGCAAAACGTTGATTTAATCACTGTTGCTCAAAT
GAACCTGTTTTTGCCCGGCGATTTTAGTGCTGTTCGCTTGCGGGTTAATGAGGCGTTTTCATTACGTCGCCTGACAGCAA
AAGTGATCGGTGAAATTGAATCCATCGCCACATTGACGGCAGCCATCGCCAGTGGAATGGGGAGCACGGTATTGCCTGAA
TCAGCTGCGCGAGCTTTATGTAGTGCGGCAAATGGCTGGATGGCGCGGATCACTACACCTTCTATGAATCTTTCGTTATC
GCTCAATATGGCAGCTAAAGGATCCCTGACACCGCAGGCTCAGGCCGTGAAAGATATTCTGCTATCGCTTATTACTTGCC
CGGCTCTGGATAAACATGAATTGCAAATGGTGAGCTAA

Upstream 100 bases:

>100_bases
GCATGGGATCCTTCGTAATGAACAAATTGGCAAGTATCTTGCAAAATCCATTCAGATAAAAAGCCAGCTAATAACTGGCA
TTCTGAACCGGAGGCCATAA

Downstream 100 bases:

>100_bases
CTGTTATTCCGCTTTGGAATAAGATGCAGGTTTTTATTATTTGTTCAGCCGGACATCAGACTTTAACAATAGCGTAATGT
CTGATGTATCCGGAGCGAAC

Product: nitrogen assimilation transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELDQQLLIRTKRGVTPTEAGKILYAHARTILRQCEQAQL
AVHNACQSLSGQVSIGLAPGTAASSMTMPLLQAVRTELPDVLVYLHENSGAALNDKLLNGELDMAVMYERSPIDGIHCQP
LLKEDLYLVGTQDCPGQNVDLITVAQMNLFLPGDFSAVRLRVNEAFSLRRLTAKVIGEIESIATLTAAIASGMGSTVLPE
SAARALCSAANGWMARITTPSMNLSLSLNMAAKGSLTPQAQAVKDILLSLITCPALDKHELQMVS

Sequences:

>Translated_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELDQQLLIRTKRGVTPTEAGKILYAHARTILRQCEQAQL
AVHNACQSLSGQVSIGLAPGTAASSMTMPLLQAVRTELPDVLVYLHENSGAALNDKLLNGELDMAVMYERSPIDGIHCQP
LLKEDLYLVGTQDCPGQNVDLITVAQMNLFLPGDFSAVRLRVNEAFSLRRLTAKVIGEIESIATLTAAIASGMGSTVLPE
SAARALCSAANGWMARITTPSMNLSLSLNMAAKGSLTPQAQAVKDILLSLITCPALDKHELQMVS
>Mature_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELDQQLLIRTKRGVTPTEAGKILYAHARTILRQCEQAQL
AVHNACQSLSGQVSIGLAPGTAASSMTMPLLQAVRTELPDVLVYLHENSGAALNDKLLNGELDMAVMYERSPIDGIHCQP
LLKEDLYLVGTQDCPGQNVDLITVAQMNLFLPGDFSAVRLRVNEAFSLRRLTAKVIGEIESIATLTAAIASGMGSTVLPE
SAARALCSAANGWMARITTPSMNLSLSLNMAAKGSLTPQAQAVKDILLSLITCPALDKHELQMVS

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=305, Percent_Identity=79.0163934426229, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI157672245, Length=244, Percent_Identity=28.6885245901639, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790399, Length=242, Percent_Identity=28.9256198347107, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788887, Length=190, Percent_Identity=34.7368421052632, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=28.1746031746032, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787601, Length=156, Percent_Identity=32.6923076923077, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788748, Length=292, Percent_Identity=25.6849315068493, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=29.6, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI145693105, Length=292, Percent_Identity=24.6575342465753, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082132, Length=254, Percent_Identity=24.8031496062992, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367136, Length=145, Percent_Identity=29.6551724137931, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789506, Length=177, Percent_Identity=23.1638418079096, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786202, Length=236, Percent_Identity=23.3050847457627, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI145693193, Length=163, Percent_Identity=26.9938650306748, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32759; Mature: 32759

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELDQQLLIRTKRGVTPTEA
CCHHHEEHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
GKILYAHARTILRQCEQAQLAVHNACQSLSGQVSIGLAPGTAASSMTMPLLQAVRTELPD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCE
VLVYLHENSGAALNDKLLNGELDMAVMYERSPIDGIHCQPLLKEDLYLVGTQDCPGQNVD
EEEEEECCCCCEECHHHCCCCCCEEEEEECCCCCCCCCCHHHHCCEEEEECCCCCCCCCC
LITVAQMNLFLPGDFSAVRLRVNEAFSLRRLTAKVIGEIESIATLTAAIASGMGSTVLPE
EEEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SAARALCSAANGWMARITTPSMNLSLSLNMAAKGSLTPQAQAVKDILLSLITCPALDKHE
HHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCH
LQMVS
HEECC
>Mature Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELDQQLLIRTKRGVTPTEA
CCHHHEEHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
GKILYAHARTILRQCEQAQLAVHNACQSLSGQVSIGLAPGTAASSMTMPLLQAVRTELPD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCE
VLVYLHENSGAALNDKLLNGELDMAVMYERSPIDGIHCQPLLKEDLYLVGTQDCPGQNVD
EEEEEECCCCCEECHHHCCCCCCEEEEEECCCCCCCCCCHHHHCCEEEEECCCCCCCCCC
LITVAQMNLFLPGDFSAVRLRVNEAFSLRRLTAKVIGEIESIATLTAAIASGMGSTVLPE
EEEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SAARALCSAANGWMARITTPSMNLSLSLNMAAKGSLTPQAQAVKDILLSLITCPALDKHE
HHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCH
LQMVS
HEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]