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The map label for this gene is ycdT [H]

Identifier: 218549243

GI number: 218549243

Start: 1949886

End: 1951277

Strand: Reverse

Name: ycdT [H]

Synonym: EFER_1903

Alternate gene names: 218549243

Gene position: 1951277-1949886 (Counterclockwise)

Preceding gene: 218549248

Following gene: 218549242

Centisome position: 42.52

GC content: 37.72

Gene sequence:

>1392_bases
ATGGATATTGTCATCTTATGGAACATTAGAAATATGAAAAAGGAATACGTCAGGATAGGCAGCACGGTTATATTCAGCTT
AATATTGGGCTTTGCTTTTATTTTAGCTAATAGCTGGTTTTTTCAACACCCCGTAGAATGGGGAGTGCCACAATCGACTT
ATTTGATTGTTATGATTGCTCTGTTCTTTATTGATACCATTGCATTTATTTTTATGCAGTTGTATTTCATCTATAATAGA
CAGGAGTTTTCAAACTGTATTCTTAGTCTGGCTTTCTTAAGCTGCCTTATCTATTTTGTCCAGACGGTTATTACTGTGCA
ACAACCAGTAGATAGTCATGCCGACATATCCATTATCACCAATGATGTTGCTATTTACTATCTTTTTCGTCAGATTAATT
TATGTTTACTAATTGTATTGGCATTGATTTGCAAAGTTTTTGAAAATCGAAAGCAACGTGCTAATAGATTGAAAAAAACA
ATGTTAATCATTAGCTTGACATGTTTATTTATTGTGCCGTTTGTTTCGCATGTAGTCAGTAGCCATCATGAAATATTGAG
TCTGGACCTCGTTGAATATGTCAGTGCGTATAAGAAAGTTGATCTGGATACTATTCATATTACAGTGATTATTGTTATGT
GGTTAGCCTTGCTTTTGGCAAATTTATATTATAATCGTTTTAGATATGATATCTGGAATGGGGTGTCAGTTATTGCTTTT
TCAGCAGTGTTATACAATATGGCAATTTTGTTTTCGAGCGGAAACAGTGCTTTTGTTTGGTATGTCAGTCGTTCAGTTGA
GATTTTTAGTAAACTCACGGTAATGTCGATTTTTATGTGTCATATTTTTCATGCCTTGAGAGTAACCAAAGATATCGCCC
ACCGGGATCCATTAACCAATATCTTTAACCGCAGCCATTTTTTCAATGAACTGAAAGGTCAGGTCAATTCTGGTGATAAT
AAACCGTTTTGCGTCATGATTATGGATATTGACCATTTCAAAAGCGTTAATGACACGTGGGGGCACCCGGAAGGAGATAA
AGTGATTAAGGCCGTAGTGGATATTATCGGTAAAAGTATCCGCCCGGACGATGTGTTAGCCCGTGTTGGCGGCGAAGAGT
TTGGCGTTATGCTGACTCATCTTGAGCGTAAAGAAGGTGAAGAATTAGCTGAGAGAATACGCAAAAATGTTGAGCAATTA
ACAGACAACAATCCGCAGTATGCAATAGCACAAAAAGTGACGATCAGTATTGGCGTAGTGGTGACGCAAGGAGATGTGCT
TCATCCCAATGATATTTACCGGCTGGCCGATAATGCCTTGTATAATGCTAAAGCAACCGGAAGAAATAAAGTCATCGTGA
CAGATGGCCGCCTGCCTGATGCTTACGCATAA

Upstream 100 bases:

>100_bases
GAATATTAAATAATTTCCATTTTAATTATATCTGGCTTTATGAATTGGCTATGAGAAAATCATATTGACATAATGTGATT
CTTATATTTCATTATATAAA

Downstream 100 bases:

>100_bases
CAGTTCCTTTCATGTTGCATATTTGACCATCCTGCAGGATGGTTTTTTTTGCCTGTAAGTATGTGTTATGCGCACTTAGC
CACACATTTTGAATGGATAA

Product: diguanylate cyclase, GGDEF protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MDIVILWNIRNMKKEYVRIGSTVIFSLILGFAFILANSWFFQHPVEWGVPQSTYLIVMIALFFIDTIAFIFMQLYFIYNR
QEFSNCILSLAFLSCLIYFVQTVITVQQPVDSHADISIITNDVAIYYLFRQINLCLLIVLALICKVFENRKQRANRLKKT
MLIISLTCLFIVPFVSHVVSSHHEILSLDLVEYVSAYKKVDLDTIHITVIIVMWLALLLANLYYNRFRYDIWNGVSVIAF
SAVLYNMAILFSSGNSAFVWYVSRSVEIFSKLTVMSIFMCHIFHALRVTKDIAHRDPLTNIFNRSHFFNELKGQVNSGDN
KPFCVMIMDIDHFKSVNDTWGHPEGDKVIKAVVDIIGKSIRPDDVLARVGGEEFGVMLTHLERKEGEELAERIRKNVEQL
TDNNPQYAIAQKVTISIGVVVTQGDVLHPNDIYRLADNALYNAKATGRNKVIVTDGRLPDAYA

Sequences:

>Translated_463_residues
MDIVILWNIRNMKKEYVRIGSTVIFSLILGFAFILANSWFFQHPVEWGVPQSTYLIVMIALFFIDTIAFIFMQLYFIYNR
QEFSNCILSLAFLSCLIYFVQTVITVQQPVDSHADISIITNDVAIYYLFRQINLCLLIVLALICKVFENRKQRANRLKKT
MLIISLTCLFIVPFVSHVVSSHHEILSLDLVEYVSAYKKVDLDTIHITVIIVMWLALLLANLYYNRFRYDIWNGVSVIAF
SAVLYNMAILFSSGNSAFVWYVSRSVEIFSKLTVMSIFMCHIFHALRVTKDIAHRDPLTNIFNRSHFFNELKGQVNSGDN
KPFCVMIMDIDHFKSVNDTWGHPEGDKVIKAVVDIIGKSIRPDDVLARVGGEEFGVMLTHLERKEGEELAERIRKNVEQL
TDNNPQYAIAQKVTISIGVVVTQGDVLHPNDIYRLADNALYNAKATGRNKVIVTDGRLPDAYA
>Mature_463_residues
MDIVILWNIRNMKKEYVRIGSTVIFSLILGFAFILANSWFFQHPVEWGVPQSTYLIVMIALFFIDTIAFIFMQLYFIYNR
QEFSNCILSLAFLSCLIYFVQTVITVQQPVDSHADISIITNDVAIYYLFRQINLCLLIVLALICKVFENRKQRANRLKKT
MLIISLTCLFIVPFVSHVVSSHHEILSLDLVEYVSAYKKVDLDTIHITVIIVMWLALLLANLYYNRFRYDIWNGVSVIAF
SAVLYNMAILFSSGNSAFVWYVSRSVEIFSKLTVMSIFMCHIFHALRVTKDIAHRDPLTNIFNRSHFFNELKGQVNSGDN
KPFCVMIMDIDHFKSVNDTWGHPEGDKVIKAVVDIIGKSIRPDDVLARVGGEEFGVMLTHLERKEGEELAERIRKNVEQL
TDNNPQYAIAQKVTISIGVVVTQGDVLHPNDIYRLADNALYNAKATGRNKVIVTDGRLPDAYA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=444, Percent_Identity=65.0900900900901, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1788085, Length=443, Percent_Identity=35.8916478555305, Blast_Score=291, Evalue=8e-80,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=37.0588235294118, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=33.7349397590361, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=35.5828220858896, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787802, Length=198, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=213, Percent_Identity=28.6384976525822, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=30.4347826086957, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=230, Percent_Identity=28.695652173913, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=31.5151515151515, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=29.8136645962733, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=166, Percent_Identity=28.3132530120482, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 53036; Mature: 53036

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIVILWNIRNMKKEYVRIGSTVIFSLILGFAFILANSWFFQHPVEWGVPQSTYLIVMIA
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHH
LFFIDTIAFIFMQLYFIYNRQEFSNCILSLAFLSCLIYFVQTVITVQQPVDSHADISIIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
NDVAIYYLFRQINLCLLIVLALICKVFENRKQRANRLKKTMLIISLTCLFIVPFVSHVVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHHEILSLDLVEYVSAYKKVDLDTIHITVIIVMWLALLLANLYYNRFRYDIWNGVSVIAF
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SAVLYNMAILFSSGNSAFVWYVSRSVEIFSKLTVMSIFMCHIFHALRVTKDIAHRDPLTN
HHHHHHHHHEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IFNRSHFFNELKGQVNSGDNKPFCVMIMDIDHFKSVNDTWGHPEGDKVIKAVVDIIGKSI
HHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
RPDDVLARVGGEEFGVMLTHLERKEGEELAERIRKNVEQLTDNNPQYAIAQKVTISIGVV
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEE
VTQGDVLHPNDIYRLADNALYNAKATGRNKVIVTDGRLPDAYA
EECCCEECCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MDIVILWNIRNMKKEYVRIGSTVIFSLILGFAFILANSWFFQHPVEWGVPQSTYLIVMIA
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHH
LFFIDTIAFIFMQLYFIYNRQEFSNCILSLAFLSCLIYFVQTVITVQQPVDSHADISIIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
NDVAIYYLFRQINLCLLIVLALICKVFENRKQRANRLKKTMLIISLTCLFIVPFVSHVVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHHEILSLDLVEYVSAYKKVDLDTIHITVIIVMWLALLLANLYYNRFRYDIWNGVSVIAF
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SAVLYNMAILFSSGNSAFVWYVSRSVEIFSKLTVMSIFMCHIFHALRVTKDIAHRDPLTN
HHHHHHHHHEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IFNRSHFFNELKGQVNSGDNKPFCVMIMDIDHFKSVNDTWGHPEGDKVIKAVVDIIGKSI
HHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
RPDDVLARVGGEEFGVMLTHLERKEGEELAERIRKNVEQLTDNNPQYAIAQKVTISIGVV
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEE
VTQGDVLHPNDIYRLADNALYNAKATGRNKVIVTDGRLPDAYA
EECCCEECCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]