Click here to switch to the map view.

The map label for this gene is ycjG [H]

Identifier: 218549001

GI number: 218549001

Start: 1696733

End: 1697857

Strand: Reverse

Name: ycjG [H]

Synonym: EFER_1649

Alternate gene names: 218549001

Gene position: 1697857-1696733 (Counterclockwise)

Preceding gene: 218549003

Following gene: 218548999

Centisome position: 37.0

GC content: 51.38

Gene sequence:

>1125_bases
TTGCGACTGCAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGGTTATCTTCCTATTTACATATAGTTAACGTCAC
CACTATTTTATGCCAACAGTCTATAACACATCAAACGCTATTTACGCCTGATCTTTTGGTATCGCATGAAGGAGAAGAGA
TGAGATCTGTAAAGGTTTATGAAGAAGCCTGGCCATTACATACCCCGTTTGTGATTGCCCGTGGCAGTCGCAGCGAAGCG
CATGTTGTGGTGGTCGAACTGGAGGAGGATGGCGTAAAAGGCGTAGGAGAATGTACGCCATATCTGCGTTATGGCGAAAG
CGATGCTTCGGTACTGGCGCAAATCATGGAGATCGTTCCGCAACTGGAAAAGGGATTAACCCGTGAAGCGCTACAGCAGT
TGTTACCGGCAGGCGCCGCACGCAACGCTGTCGATTGTGCGTTATGGGATCTTCAGGCCCGGCAGCAACAGCAAACACTG
GAAGAGTTACTGGGCATTGAACTAAATTCAACCATTACTACGGCACAGACTGTCGTGATTGGTACTCCCGAGCAGATGGC
CACCAGTGCTGCCGCTTTGTGGAATAAGGGCGCGACGTTACTGAAGGTGAAACTTGATAGTCATTTGATCAGTGAGCGAA
TGGTGGCAATACGTGCAGCAGCACCTGAAGCCACGATCATTGTCGATGCCAATAAGTCCTGGCGCGCTGAGGGACTGGCG
GCACGTTGCCAGTTGCTGGCAGATCTGGGCGTGGCAATGCTTGAACAACCGCTTCCTGCCCAGGACGACGCCGCACTGGA
GAACTTCATTCATCCTCTGCCAATTTGTGCTGATGAGAGTTGCCATACCCGTAGCAACCTTAAAGCGCTGAAAGGGCGTT
ATGAAATGGTCAACATCAAGCTGGATAAAACTGGCGGTTTGACTGAGGCACTGGCACTGGCCACCGAAGCTAAGGCGCAG
GGATTCAGTCTGATGCTAGGTTGTATGCTCTGCACTTCACGTGCGATTAGCGCCGCTTTGCCGCTTGTTCCTCAGGTCAG
TTTTGCTGATCTTGATGGCCCCACCTGGCTGGCAATGGATGTGGAGCCACATCTGCATTTTACGACCGGACAAGTGCATC
TTTAG

Upstream 100 bases:

>100_bases
ACTTTGCGTTTGCCAGCAAACTGACTCAGTGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGGCTGCGCTTTGCT
ACCCGCCTGAGGAATGGAGT

Downstream 100 bases:

>100_bases
GATGCCAGCGTAGTAGGTTGGACATCGCGAACAGGTACTTCTCGCTGGCTTCATCTGATGAGATGGCCGGAAACTCGGCG
GTGATACAGGGTAAATTGAG

Product: L-Ala-D/L-Glu epimerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRLQPGCPGNERFVTWLSSYLHIVNVTTILCQQSITHQTLFTPDLLVSHEGEEMRSVKVYEEAWPLHTPFVIARGSRSEA
HVVVVELEEDGVKGVGECTPYLRYGESDASVLAQIMEIVPQLEKGLTREALQQLLPAGAARNAVDCALWDLQARQQQQTL
EELLGIELNSTITTAQTVVIGTPEQMATSAAALWNKGATLLKVKLDSHLISERMVAIRAAAPEATIIVDANKSWRAEGLA
ARCQLLADLGVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEAKAQ
GFSLMLGCMLCTSRAISAALPLVPQVSFADLDGPTWLAMDVEPHLHFTTGQVHL

Sequences:

>Translated_374_residues
MRLQPGCPGNERFVTWLSSYLHIVNVTTILCQQSITHQTLFTPDLLVSHEGEEMRSVKVYEEAWPLHTPFVIARGSRSEA
HVVVVELEEDGVKGVGECTPYLRYGESDASVLAQIMEIVPQLEKGLTREALQQLLPAGAARNAVDCALWDLQARQQQQTL
EELLGIELNSTITTAQTVVIGTPEQMATSAAALWNKGATLLKVKLDSHLISERMVAIRAAAPEATIIVDANKSWRAEGLA
ARCQLLADLGVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEAKAQ
GFSLMLGCMLCTSRAISAALPLVPQVSFADLDGPTWLAMDVEPHLHFTTGQVHL
>Mature_374_residues
MRLQPGCPGNERFVTWLSSYLHIVNVTTILCQQSITHQTLFTPDLLVSHEGEEMRSVKVYEEAWPLHTPFVIARGSRSEA
HVVVVELEEDGVKGVGECTPYLRYGESDASVLAQIMEIVPQLEKGLTREALQQLLPAGAARNAVDCALWDLQARQQQQTL
EELLGIELNSTITTAQTVVIGTPEQMATSAAALWNKGATLLKVKLDSHLISERMVAIRAAAPEATIIVDANKSWRAEGLA
ARCQLLADLGVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEAKAQ
GFSLMLGCMLCTSRAISAALPLVPQVSFADLDGPTWLAMDVEPHLHFTTGQVHL

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=321, Percent_Identity=86.2928348909657, Blast_Score=560, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 40715; Mature: 40715

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQPGCPGNERFVTWLSSYLHIVNVTTILCQQSITHQTLFTPDLLVSHEGEEMRSVKVY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHEEECCCCHHHHEEHH
EEAWPLHTPFVIARGSRSEAHVVVVELEEDGVKGVGECTPYLRYGESDASVLAQIMEIVP
HHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
QLEKGLTREALQQLLPAGAARNAVDCALWDLQARQQQQTLEELLGIELNSTITTAQTVVI
HHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
GTPEQMATSAAALWNKGATLLKVKLDSHLISERMVAIRAAAPEATIIVDANKSWRAEGLA
ECCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHEECCCCEEEEEECCCCCCHHHHH
ARCQLLADLGVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSNLKALKGRYEMVNIK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEE
LDKTGGLTEALALATEAKAQGFSLMLGCMLCTSRAISAALPLVPQVSFADLDGPTWLAMD
EECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEEE
VEPHLHFTTGQVHL
CCCCEEEECCCEEC
>Mature Secondary Structure
MRLQPGCPGNERFVTWLSSYLHIVNVTTILCQQSITHQTLFTPDLLVSHEGEEMRSVKVY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHEEECCCCHHHHEEHH
EEAWPLHTPFVIARGSRSEAHVVVVELEEDGVKGVGECTPYLRYGESDASVLAQIMEIVP
HHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
QLEKGLTREALQQLLPAGAARNAVDCALWDLQARQQQQTLEELLGIELNSTITTAQTVVI
HHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
GTPEQMATSAAALWNKGATLLKVKLDSHLISERMVAIRAAAPEATIIVDANKSWRAEGLA
ECCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHEECCCCEEEEEECCCCCCHHHHH
ARCQLLADLGVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSNLKALKGRYEMVNIK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEE
LDKTGGLTEALALATEAKAQGFSLMLGCMLCTSRAISAALPLVPQVSFADLDGPTWLAMD
EECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEEE
VEPHLHFTTGQVHL
CCCCEEEECCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]