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The map label for this gene is lsrG

Identifier: 218548914

GI number: 218548914

Start: 1591117

End: 1591407

Strand: Reverse

Name: lsrG

Synonym: EFER_1559

Alternate gene names: 218548914

Gene position: 1591407-1591117 (Counterclockwise)

Preceding gene: 218548915

Following gene: 218548912

Centisome position: 34.68

GC content: 46.39

Gene sequence:

>291_bases
ATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCT
GGGCTCGGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACG
AAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCAAAACTGGAATCT
TTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGATTGATGCCGTGA

Upstream 100 bases:

>100_bases
AAGCCGTACAGGGGGTGGTACACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTG
CGGATTTAAGGAGAAGAATT

Downstream 100 bases:

>100_bases
GGCGAATTTATCCGGTGCGCCGTAAAACCCCGTCCTTCAGGGCGGGGATATAAGGCGCGGTTTTTCACCTAACCAGGTGT
TTGCTGTTGTTCAATGTATT

Product: autoinducer-2 (AI-2) modifying protein LsrG

Products: NA

Alternate protein names: AI-2-degrading protein lsrG

Number of amino acids: Translated: 96; Mature: 96

Protein sequence:

>96_residues
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLES
LMTGPRKKRLFNGLMP

Sequences:

>Translated_96_residues
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLES
LMTGPRKKRLFNGLMP
>Mature_96_residues
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLES
LMTGPRKKRLFNGLMP

Specific function: Involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle. Catalyzes the cleavage of phosphorylated 4,5- dihydroxy-2,3-pentanedione (P-DPD) to 2-phosphoglycolic acid (PG) and another

COG id: COG1359

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lsrG family

Homologues:

Organism=Escherichia coli, GI1787797, Length=96, Percent_Identity=100, Blast_Score=202, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSRG_ECO57 (P64462)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85723
- PIR:   E90894
- RefSeq:   NP_287636.1
- RefSeq:   NP_310152.1
- ProteinModelPortal:   P64462
- SMR:   P64462
- EnsemblBacteria:   EBESCT00000026347
- EnsemblBacteria:   EBESCT00000056354
- GeneID:   917322
- GeneID:   960648
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2187
- KEGG:   ecs:ECs2125
- GeneTree:   EBGT00050000011886
- HOGENOM:   HBG693987
- OMA:   KKTPHYL
- ProtClustDB:   PRK10486
- BioCyc:   ECOL83334:ECS2125-MONOMER
- GO:   GO:0005737
- InterPro:   IPR007138
- InterPro:   IPR011008

Pfam domain/function: PF03992 ABM; SSF54909 Dimer_A_B_barrel

EC number: NA

Molecular weight: Translated: 11255; Mature: 11255

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFYIYEAYKDED
CEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCC
AVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP
CEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFYIYEAYKDED
CEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCC
AVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP
CEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796