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The map label for this gene is benA [H]

Identifier: 218548839

GI number: 218548839

Start: 1518558

End: 1519940

Strand: Direct

Name: benA [H]

Synonym: EFER_1482

Alternate gene names: 218548839

Gene position: 1518558-1519940 (Clockwise)

Preceding gene: 218548838

Following gene: 218548840

Centisome position: 33.09

GC content: 46.42

Gene sequence:

>1383_bases
ATGCAAAAAACTCTCTCAACATTAAAAGATAAAATCAGCAATGCATTAATTGTTGATCGTGAAAATCATATTTATCGTTG
CCATCGGTCTATATTTACCGATCAGCAATTGTTTGATTTTGAAATGAAGCATATTTTTGAAGGTAACTGGGTATTCCTCG
CCCATGAAAGCCAAATTCCCGAAGCAGGGGATTATTATACTTTAACGCTGGGACGTCAGCCGGTCATTATTACCCGCGAT
AAAAAAGGTGAGCTTCATGCCTTAATCAATAGTTGTGCTCACCGTGGCGCGATGTTATGTCGACGCAAGCGGGGAAATAA
AAACTCATTTACCTGTCCATTTCATGGCTGGACATTTAGTAATAACGGCAAATTATTAAAAGCAAAAGATGAAAGTACCG
GCGCGTATCCTCCAACATTTAAACATGAAGGTTCCCACGATTTACAGAAATTGCCCCGTTTCCAGTCGTATCGCGGATTT
CTGTTCGGTAGCCTGAATGACGATGTTATGTCGCTGGAAGAGTATCTCGGTGAAACCTGCAAAATCATTGATTTGATCGT
TGATCAAGCTCCGGAAGGGCTGGAAGTTCTCAATGGATCGTCGAGCTATGTTTATGAAGGGAACTGGAAGCTCGGTGCGG
AAAACGGTGCTGACGGTTACCACGTTAGCGTGGTGCACTGGAACTACTCGTCAACGATGTCACGGCGAAATTATGAAGCG
GAAGGTACCCGCGCTGTAGATGCCAATGGCTGGTCAAAAAGTATTGGTGGCGGATATGGCTTTGATAACGGTCACATGTT
GCTCTGGACTCGTACGTTAAACCCGGAAGTGCGTCCGGTTTATGACCATCGGGAACGTTTACAGGCAGAATTTGGCGAAC
TGCGTGCTGACCAAATGGTGAACGAAACGCGTAATCTCTGTATCTATCCCAATGTTTATTTGATGGATCAGTTTTCCACG
CAGATCCGCGTAATCCGCCCCATTGCAGTTGATAAAACCGAAGTCACTATCTGGTGTTTTGCGCCAAAAGGCGAATCTGA
ACAGGCTCGCGCATTGCGTATTCGTCAGTATGAAGATTTCTTTAATGTCAGCGGGATGGGCACACCAGATGATCTGGAAG
AATTCAGCGCCTGTCAGCGCGGTTATCTTGGTGAAAACCTGCCATGGAGCGATCTCAGCCGTGGTGCTCTGCATTGGGTT
GATGGCCCGGACGAACATGCGCAACACGCAGGATTTACGCCATTGCTCAGCGGCGTGAAATCGGAAGATGAAGCACTGTA
TATCGCCCATCACCATCACTGGCAAAACGTCATGCTGGCGGCACTGGAAAAAGAACAGATGTGTTATGACCAGTCAATTA
CTCAGCGTGTGGAGGTGGCCTGA

Upstream 100 bases:

>100_bases
CGCCTGCGCGCACAAGAATAAATTCTCCTTTCGCGGTATTACGTTGAGGTAATACCGCCGTTTTTATTTTATATCTGGTT
TATCCCGGAGGGCTAAGTTC

Downstream 100 bases:

>100_bases
TGTTAACCCTCGAACAAGTCCGCCAGTTTCTCTATTACGAAGCCCGACTGCTGGATGATCGCCAGTGGGATGAGTGGCTG
AACTGCTATAGCCCAAAAGT

Product: Benzoate 1,2-dioxygenase subunit alpha

Products: NA

Alternate protein names: 2-chlorobenzoate 1,2-dioxygenase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MQKTLSTLKDKISNALIVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIPEAGDYYTLTLGRQPVIITRD
KKGELHALINSCAHRGAMLCRRKRGNKNSFTCPFHGWTFSNNGKLLKAKDESTGAYPPTFKHEGSHDLQKLPRFQSYRGF
LFGSLNDDVMSLEEYLGETCKIIDLIVDQAPEGLEVLNGSSSYVYEGNWKLGAENGADGYHVSVVHWNYSSTMSRRNYEA
EGTRAVDANGWSKSIGGGYGFDNGHMLLWTRTLNPEVRPVYDHRERLQAEFGELRADQMVNETRNLCIYPNVYLMDQFST
QIRVIRPIAVDKTEVTIWCFAPKGESEQARALRIRQYEDFFNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGALHWV
DGPDEHAQHAGFTPLLSGVKSEDEALYIAHHHHWQNVMLAALEKEQMCYDQSITQRVEVA

Sequences:

>Translated_460_residues
MQKTLSTLKDKISNALIVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIPEAGDYYTLTLGRQPVIITRD
KKGELHALINSCAHRGAMLCRRKRGNKNSFTCPFHGWTFSNNGKLLKAKDESTGAYPPTFKHEGSHDLQKLPRFQSYRGF
LFGSLNDDVMSLEEYLGETCKIIDLIVDQAPEGLEVLNGSSSYVYEGNWKLGAENGADGYHVSVVHWNYSSTMSRRNYEA
EGTRAVDANGWSKSIGGGYGFDNGHMLLWTRTLNPEVRPVYDHRERLQAEFGELRADQMVNETRNLCIYPNVYLMDQFST
QIRVIRPIAVDKTEVTIWCFAPKGESEQARALRIRQYEDFFNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGALHWV
DGPDEHAQHAGFTPLLSGVKSEDEALYIAHHHHWQNVMLAALEKEQMCYDQSITQRVEVA
>Mature_460_residues
MQKTLSTLKDKISNALIVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIPEAGDYYTLTLGRQPVIITRD
KKGELHALINSCAHRGAMLCRRKRGNKNSFTCPFHGWTFSNNGKLLKAKDESTGAYPPTFKHEGSHDLQKLPRFQSYRGF
LFGSLNDDVMSLEEYLGETCKIIDLIVDQAPEGLEVLNGSSSYVYEGNWKLGAENGADGYHVSVVHWNYSSTMSRRNYEA
EGTRAVDANGWSKSIGGGYGFDNGHMLLWTRTLNPEVRPVYDHRERLQAEFGELRADQMVNETRNLCIYPNVYLMDQFST
QIRVIRPIAVDKTEVTIWCFAPKGESEQARALRIRQYEDFFNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGALHWV
DGPDEHAQHAGFTPLLSGVKSEDEALYIAHHHHWQNVMLAALEKEQMCYDQSITQRVEVA

Specific function: Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=380, Percent_Identity=28.4210526315789, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788103, Length=211, Percent_Identity=28.436018957346, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.13 [H]

Molecular weight: Translated: 52577; Mature: 52577

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTLSTLKDKISNALIVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIP
CCHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHEECCCEEEEEECCCCC
EAGDYYTLTLGRQPVIITRDKKGELHALINSCAHRGAMLCRRKRGNKNSFTCPFHGWTFS
CCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEC
NNGKLLKAKDESTGAYPPTFKHEGSHDLQKLPRFQSYRGFLFGSLNDDVMSLEEYLGETC
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCEEEECCCHHHHHHHHHHCHHH
KIIDLIVDQAPEGLEVLNGSSSYVYEGNWKLGAENGADGYHVSVVHWNYSSTMSRRNYEA
HHHHHHHHCCCCCHHEECCCCCEEEECCEEECCCCCCCCEEEEEEEECCHHHHHHCCCCC
EGTRAVDANGWSKSIGGGYGFDNGHMLLWTRTLNPEVRPVYDHRERLQAEFGELRADQMV
CCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NETRNLCIYPNVYLMDQFSTQIRVIRPIAVDKTEVTIWCFAPKGESEQARALRIRQYEDF
HHCCCEEECCCEEEEECCCCCEEEEEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHH
FNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGALHWVDGPDEHAQHAGFTPLLSGVK
HCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCEEEECCCCHHHHHHCCCHHHHHCCC
SEDEALYIAHHHHWQNVMLAALEKEQMCYDQSITQRVEVA
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKTLSTLKDKISNALIVDRENHIYRCHRSIFTDQQLFDFEMKHIFEGNWVFLAHESQIP
CCHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHEECCCEEEEEECCCCC
EAGDYYTLTLGRQPVIITRDKKGELHALINSCAHRGAMLCRRKRGNKNSFTCPFHGWTFS
CCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEC
NNGKLLKAKDESTGAYPPTFKHEGSHDLQKLPRFQSYRGFLFGSLNDDVMSLEEYLGETC
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCEEEECCCHHHHHHHHHHCHHH
KIIDLIVDQAPEGLEVLNGSSSYVYEGNWKLGAENGADGYHVSVVHWNYSSTMSRRNYEA
HHHHHHHHCCCCCHHEECCCCCEEEECCEEECCCCCCCCEEEEEEEECCHHHHHHCCCCC
EGTRAVDANGWSKSIGGGYGFDNGHMLLWTRTLNPEVRPVYDHRERLQAEFGELRADQMV
CCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NETRNLCIYPNVYLMDQFSTQIRVIRPIAVDKTEVTIWCFAPKGESEQARALRIRQYEDF
HHCCCEEECCCEEEEECCCCCEEEEEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHH
FNVSGMGTPDDLEEFSACQRGYLGENLPWSDLSRGALHWVDGPDEHAQHAGFTPLLSGVK
HCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCEEEECCCCHHHHHHCCCHHHHHCCC
SEDEALYIAHHHHWQNVMLAALEKEQMCYDQSITQRVEVA
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7530709; 1370284 [H]