Click here to switch to the map view.

The map label for this gene is pntB [H]

Identifier: 218548796

GI number: 218548796

Start: 1476290

End: 1477678

Strand: Direct

Name: pntB [H]

Synonym: EFER_1435

Alternate gene names: 218548796

Gene position: 1476290-1477678 (Clockwise)

Preceding gene: 218548795

Following gene: 218548797

Centisome position: 32.17

GC content: 49.39

Gene sequence:

>1389_bases
ATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCAATCCTGTTTATTTTTAGTCTGGCGGGGCTTTCAAAACA
CGAAACTTCGCAACGGGGAAATAACTTTGGTATAGCCGGGATGGCGATAGCGTTAATCGCTACCATCTTTGGGCCGGATA
CAGGCAATGTTGCATGGATTCTGGTGGCGATGATCATCGGTGGTGCAATTGGTATCCGCCTGGCGAAAAAGGTTGAAATG
ACCGAGATGCCAGAACTGGTGGCGATTCTGCACAGCTTCGTAGGTCTGGCGGCGGTGCTGGTTGGTTTTAACAGTTACCT
GCATCATGATGCTGGCATGGAGCCAGTACTGGTCAATATTCACCTGACGGAGGTATTCCTCGGTATCTTTATCGGTGCGG
TAACGTTCACCGGTTCGGTTGTGGCGTTCGGTAAATTGTGCGGAAAAATCTCCTCAAAACCGCTGATGCTACCAAATCGT
CATAAAATGAACCTTGCGGCACTGGTTGTTTCCTTCCTGTTACTGCTTGTTTTCGTTCGCACCGAAAGTGTTGGTCTGCA
AGTGTTGGCACTGCTGGTGATGACCGTGATCGCACTGGTATTTGGCTGGCATTTAGTTGCATCTATCGGCGGGGCGGATA
TGCCTGTCGTAGTATCAATGCTCAACTCATACTCTGGTTGGGCTGCGGCAGCCGCAGGCTTTATGCTGAGTAACGACCTG
TTAATTGTTACTGGTGCGCTGGTGGGGTCTTCTGGTGCCATCCTCTCTTACATTATGTGTAAGGCGATGAATCGCTCATT
CATCAGCGTTATCGCCGGTGGCTTTGGTACAGACGGAATCTCCACAGGTGATGATCAGGAAGTTGGTGAACATCGTGAAA
TCACTGCGGAAGAAACTGCTGAACTGCTGAAGAACTCACATTCAGTGATTATCACTCCAGGTTACGGCATGGCAGTGGCA
CAGGCTCAATATCCGGTAGCGGAAATCACGGAGAAACTGCGTGCCCGTGGCATCAACGTGCGTTTTGGGATTCACCCGGT
AGCAGGGCGTTTGCCTGGCCATATGAATGTGTTACTGGCAGAAGCGAAAGTGCCGTACGACATCGTGCTGGAAATGGACG
AAATCAATGATGATTTTGCCGACACGGATACCGTACTGGTCATTGGTGCTAACGACACGGTTAACCCAGCGGCACAGGAC
GATCCTAAGAGTCCAATTGCCGGGATGCCAGTTCTCGAAGTGTGGAAAGCGCAAAACGTAATTGTCTTTAAACGTTCGAT
GAATACCGGTTATGCCGGGGTGCAAAACCCGCTGTTCTTTAAAGAAAATACTCATATGCTGTTTGGTGATGCTAAAGCCA
GCGTGGATGCAATATTGAAAGCACTTTAA

Upstream 100 bases:

>100_bases
AGCTTTATTGCGGTGCTTATCGCCAGCATTAATATTTTCGGTGGTTTCACTGTAACTCAGCGTATGCTGAAAATGTTCCG
CAAAAATTAAGGGGTAACAT

Downstream 100 bases:

>100_bases
GTAAACCTCAGTGCACAACGGCCTCTTCGGGGGCCGTTTTTTTAAGCTCATGTGATGTAAATACGCAGATTTAATTTTTG
CTCTCATGAAATGCAGCTAA

Product: pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Pyridine nucleotide transhydrogenase subunit beta [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSGGLVTAAYIVAAILFIFSLAGLSKHETSQRGNNFGIAGMAIALIATIFGPDTGNVAWILVAMIIGGAIGIRLAKKVEM
TEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMEPVLVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
HKMNLAALVVSFLLLLVFVRTESVGLQVLALLVMTVIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDL
LIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGISTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVA
QAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQD
DPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL

Sequences:

>Translated_462_residues
MSGGLVTAAYIVAAILFIFSLAGLSKHETSQRGNNFGIAGMAIALIATIFGPDTGNVAWILVAMIIGGAIGIRLAKKVEM
TEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMEPVLVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
HKMNLAALVVSFLLLLVFVRTESVGLQVLALLVMTVIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDL
LIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGISTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVA
QAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQD
DPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL
>Mature_461_residues
SGGLVTAAYIVAAILFIFSLAGLSKHETSQRGNNFGIAGMAIALIATIFGPDTGNVAWILVAMIIGGAIGIRLAKKVEMT
EMPELVAILHSFVGLAAVLVGFNSYLHHDAGMEPVLVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNRH
KMNLAALVVSFLLLLVFVRTESVGLQVLALLVMTVIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLL
IVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGISTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQ
AQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQDD
PKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=453, Percent_Identity=51.4348785871965, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI122939153, Length=453, Percent_Identity=51.4348785871965, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI1787886, Length=462, Percent_Identity=97.4025974025974, Blast_Score=910, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=457, Percent_Identity=49.671772428884, Blast_Score=426, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 48836; Mature: 48705

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEECCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHCC
>Mature Secondary Structure 
SGGLVTAAYIVAAILFIFSLAGLSKHETSQRGNNFGIAGMAIALIATIFGPDTGNVAWI
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
LVAMIIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMEPVLVNI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
HLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNRHKMNLAALVVSFLLLLVFVR
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TESVGLQVLALLVMTVIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCE
LIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGISTGDDQEVGEHREITAEETA
EEEEEEHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHH
ELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLA
HHHHCCCCEEEECCCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCHHCCCCCCCCEEEEE
EAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQDDPKSPIAGMPVLEVWKAQNV
CCCCCEEEEEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCE
IVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL
EEEEECCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]