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The map label for this gene is pps [H]

Identifier: 218548725

GI number: 218548725

Start: 1396452

End: 1398830

Strand: Direct

Name: pps [H]

Synonym: EFER_1362

Alternate gene names: 218548725

Gene position: 1396452-1398830 (Clockwise)

Preceding gene: 218548721

Following gene: 218548728

Centisome position: 30.43

GC content: 51.37

Gene sequence:

>2379_bases
ATGTCCAACAATGGCTCGTCACCGCTGGTGCTTTGGTATAACCAACTCGGCATGAATGATGTAGACAGAGTTGGGGGCAA
AAATGCCTCCCTGGGTGAAATGATTACTAACCTTTCTGGAATGGGCGTATCCGTACCGAATGGTTTTGCCACAACCGCCG
ATGCGTTTAATCAGTTTCTCGATCAGAGCGGTGTAAACCAGCGTATCTATGAACTGCTGGATAAAACAGATATTGACGAT
GTCTCGCAACTTGCTAAAGCAGGTGCGCAGATTCGCCAGTGGATTATCGACACTCCTTTCCAGCCAGAGCTGGAAAATGC
TATTCGCGAAGCTTATGCCCAGCTTTCTGCGGATGACGAACATGCATCTTTCGCCGTGCGCTCGTCTGCTACTGCAGAAG
ATATGCCGGATGCGTCCTTTGCGGGCCAACAAGAAACTTTTCTCAATGTGCAGGGCTTCGATGCCGTCCTGATTGCGGTA
AAACACGTATTTGCCTCCCTGTTTAATGATCGCGCCATTTCTTATCGCGTACACCAGGGATATGACCATCGTGGTGTCGC
TTTGTCTGCTGGTGTTCAACGGATGGTGCGCTCAGATCTGGCATCCTCTGGCGTGATGTTCTCCATTGATACCGAATCGG
GTTTCGATCAGGTGGTATTTATCACTTCGGCCTGGGGGCTGGGTGAGATGGTGGTGCAGGGCGCTGTTAACCCGGATGAA
TTTTACGTACACAAGCCGACGCTGGCCGCAAATCGTCCGTCCATTGTTCGCCGCACCATGGGGTCGAAAAAAATTCGGAT
GGTCTACGCATCAACCCAGGAACATGGCAAGCAAGTCAGAATTGAAGATGTACCGCAAGAGCAACGAGACATTTTCTCGC
TGACCAACGATGAAGTGCAGGAACTGGCGAAACAGGCTGTTCAGATTGAAAAACACTATGGACGTCCGATGGATATCGAA
TGGGCCAAAGATGGTCATACTGGCAAATTGTTCATCGTCCAGGCGCGTCCGGAAACAGTGCGCTCCCGTGGTCAGGTTAT
GGAGCGTTATACCCTTCACGCGCAAGGCAAGATCATTGCGGAAGGGCGTGCAATTGGTCACCGCATTGGTGCTGGAACAG
TGAAAGTGATTCAGGACATTAGTGAAATGAATCGCATTGAGCCAGGTGATGTTCTGGTCACTGACATGACCGATCCCGAC
TGGGAGCCGATCATGAAAAAAGCAGCAGCCATCGTAACTAACCGAGGCGGCAGAACCTGTCACGCAGCGATTATTGCCCG
TGAGCTGGGAATTCCGGCAGTGGTTGGGTGTGGCGATGCTACTGAACGGATGAAAGATGGCGAGCAGGTAACGGTTTCTT
GCGCAGAAGGCGACACTGGCTATGTTTATGCCGATCTTCTTGATTTCAGTGTGAAAAGTTCCAGCGTAGATACCATGCCT
GACTTACCGTTGAAAGTGATGATGAACGTTGGTAACCCGGATCGTGCGTTTGACTTCGCTTGTCTGCCAAATGAAGGCGT
TGGCCTGGCGCGACTAGAGTTCATCATTAACCGTATGATTGGTGTTCACCCGCGCGCACTGCTGGAATTTGACGATCAGG
AACCGCAGCTGCAAAAAGAAATCCGCGAGATGATGAAAGGTTTTGATTCACCGCGTGAGTTCTATGTCGGTCGTCTGACA
GAAGGTATTGCTACGCTGGGAGCGGCGTTTTATCCGAAGCGTGTCATCGTACGTCTCTCTGACTTTAAATCGAATGAGTA
CGCTAACCTCGTTGGTGGCGAACGTTATGAACCGGAAGAAGAGAACCCGATGTTGGGCTTCCGTGGTGCAGGGCGTTATG
TTTCCGACAGCTTCCGCGACTGCTTTGCGCTGGAGTGTGAAGCGGTGAAACGCGTACGCAACGACATGGGGCTGACCAAC
GTTGAAATCATGATCCCATTTGTGCGCACCGTAGACCAGGCGAAAGCGGTAGTTGAGGAGTTGGCGCGCCAGGGGCTGAA
ACGCGGCGAGAACGGTCTTAAGATCATTATGATGTGTGAGATCCCGTCTAATGCCTTGTTAGCGGATCAGTTCCTGCAAT
ACTTCGACGGTTTCTCAATCGGTTCTAACGATATGACGCAACTGGCACTGGGCCTGGATCGTGACTCTGGCGTAGTCTCT
GAGCTGTTTGATGAGCGTAACGAGGCGGTGAAAGCACTGCTGTCGATGGCTATTCGTGCAGCTAAAAAACAGGGTAAATA
CGTCGGGATTTGTGGGCAGGGGCCTTCTGATCATGAAGACTTCGCCGCCTGGCTGATGGAAGAGGGAATCGACAGCTTGT
CGCTTAACCCGGATACCGTCGTTCAGACATGGTTGAGCCTTGCAGAGCTGAAAAAGTAA

Upstream 100 bases:

>100_bases
TGAACGATTCACTGTTTTTTTCTGCGAGACAAATATGGCAGGATAATTACGCAGTAAGATGGTTCTTAAATCCGTTCATT
TATCACAAAAGGATTGTTCG

Downstream 100 bases:

>100_bases
TTTAACAACGTGATCGACAAGCAGTGTTGTCACAATTGTGGCAACACTGCTTTTTTTATGGTGCGAGGAGTTAACAAAGA
CGCTCTAAATCGTGGGCGTA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 792; Mature: 791

Protein sequence:

>792_residues
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDD
VSQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDEHASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLIAV
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLAANRPSIVRRTMGSKKIRMVYASTQEHGKQVRIEDVPQEQRDIFSLTNDEVQELAKQAVQIEKHYGRPMDIE
WAKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIAEGRAIGHRIGAGTVKVIQDISEMNRIEPGDVLVTDMTDPD
WEPIMKKAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEQVTVSCAEGDTGYVYADLLDFSVKSSSVDTMP
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQKEIREMMKGFDSPREFYVGRLT
EGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPEEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTN
VEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLADQFLQYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNEAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK

Sequences:

>Translated_792_residues
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDD
VSQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDEHASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLIAV
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLAANRPSIVRRTMGSKKIRMVYASTQEHGKQVRIEDVPQEQRDIFSLTNDEVQELAKQAVQIEKHYGRPMDIE
WAKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIAEGRAIGHRIGAGTVKVIQDISEMNRIEPGDVLVTDMTDPD
WEPIMKKAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEQVTVSCAEGDTGYVYADLLDFSVKSSSVDTMP
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQKEIREMMKGFDSPREFYVGRLT
EGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPEEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTN
VEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLADQFLQYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNEAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK
>Mature_791_residues
SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDV
SQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDEHASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLIAVK
HVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDEF
YVHKPTLAANRPSIVRRTMGSKKIRMVYASTQEHGKQVRIEDVPQEQRDIFSLTNDEVQELAKQAVQIEKHYGRPMDIEW
AKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIAEGRAIGHRIGAGTVKVIQDISEMNRIEPGDVLVTDMTDPDW
EPIMKKAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEQVTVSCAEGDTGYVYADLLDFSVKSSSVDTMPD
LPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQKEIREMMKGFDSPREFYVGRLTE
GIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPEEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNV
EIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLADQFLQYFDGFSIGSNDMTQLALGLDRDSGVVSE
LFDERNEAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=792, Percent_Identity=97.4747474747475, Blast_Score=1607, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=449, Percent_Identity=26.7260579064588, Blast_Score=113, Evalue=6e-26,
Organism=Escherichia coli, GI1788726, Length=192, Percent_Identity=35.4166666666667, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=31.5789473684211, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1789193, Length=410, Percent_Identity=25.3658536585366, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17564524, Length=331, Percent_Identity=25.9818731117825, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87441; Mature: 87310

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
CCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHH
DQSGVNQRIYELLDKTDIDDVSQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
HASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLIAVKHVFASLFNDRAISYRVHQG
HHHEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
CCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCC
FYVHKPTLAANRPSIVRRTMGSKKIRMVYASTQEHGKQVRIEDVPQEQRDIFSLTNDEVQ
EEEECCCCCCCCHHHHHHHCCCCEEEEEEECCHHCCCEEEECCCCHHHHHHHHCCCHHHH
ELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIA
HHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEE
EGRAIGHRIGAGTVKVIQDISEMNRIEPGDVLVTDMTDPDWEPIMKKAAAIVTNRGGRTC
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCH
HAAIIARELGIPAVVGCGDATERMKDGEQVTVSCAEGDTGYVYADLLDFSVKSSSVDTMP
HHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEEECCCCCEEEEEHHHHHCCCCCCCCCC
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQKE
CCCHHEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEECCCCCHHHHHH
IREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPEE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCCC
ENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
LARQGLKRGENGLKIIMMCEIPSNALLADQFLQYFDGFSIGSNDMTQLALGLDRDSGVVS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHH
ELFDERNEAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
VQTWLSLAELKK
HHHHHHHHHHCC
>Mature Secondary Structure 
SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
CCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHH
DQSGVNQRIYELLDKTDIDDVSQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
HASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLIAVKHVFASLFNDRAISYRVHQG
HHHEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
CCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCC
FYVHKPTLAANRPSIVRRTMGSKKIRMVYASTQEHGKQVRIEDVPQEQRDIFSLTNDEVQ
EEEECCCCCCCCHHHHHHHCCCCEEEEEEECCHHCCCEEEECCCCHHHHHHHHCCCHHHH
ELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIA
HHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEE
EGRAIGHRIGAGTVKVIQDISEMNRIEPGDVLVTDMTDPDWEPIMKKAAAIVTNRGGRTC
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCH
HAAIIARELGIPAVVGCGDATERMKDGEQVTVSCAEGDTGYVYADLLDFSVKSSSVDTMP
HHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEEECCCCCEEEEEHHHHHCCCCCCCCCC
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQKE
CCCHHEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEECCCCCHHHHHH
IREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPEE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCCC
ENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
LARQGLKRGENGLKIIMMCEIPSNALLADQFLQYFDGFSIGSNDMTQLALGLDRDSGVVS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHH
ELFDERNEAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
VQTWLSLAELKK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]