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The map label for this gene is chbR [H]

Identifier: 218548696

GI number: 218548696

Start: 1368091

End: 1368933

Strand: Direct

Name: chbR [H]

Synonym: EFER_1332

Alternate gene names: 218548696

Gene position: 1368091-1368933 (Clockwise)

Preceding gene: 218548695

Following gene: 218548697

Centisome position: 29.81

GC content: 41.4

Gene sequence:

>843_bases
ATGATGCAACCGCAGATTAGCGCACCGGAAATTGCCACGGCCCGTGAGCAACAACTGTTTAATGGTAAGAATTTCCATGT
GTTTATCTACAATAAAACTGAAAGTATCAGTGGGTTGCATCAGCATGACTACTACGAGTTCACCCTGGTTTTAACTGGCC
GCTACTACCAGGAGATTAACGGTAAACGTGTATTGCTGGAGCGTGGTGATTTTGTGTTTATCCCGCTGGGATCACATCAC
CAGAGCTTTTATGAGTTTGGCGCTACACGTATTTTGAATGTTGGTATCAGTAAGCGGTTTTTTGAACAGCATTATTTGCC
TTTATTGCCATTCTGTTTTGTTGCTTCACAGGTCTATCGCGCCAACAGTGCTTTTATGACCTATGTCGAAACGGTTATCG
CGTCACTTAATTTTCGTGATACCGGGCTGGAAGAGTTTGTTGAACTGGTTTCGTTTTATATTATCAACCGGCTGCGTCAT
TATCGTGAAGAACAAGTGTATGACGATATTCCCCAGTGGCTAAAAGTGACTGTGGAAATAATGCACGATAAAACACAGTT
CGGAGATCATGCGCTGGAAAACATGGTTAAGCTATCTGGTAAATCTCAGGAGTATTTAACCCGTGCTACACAGCGTTATT
ACCGCAAAACGCCGATGCAAATGATTAATGAAATTCGCATTAATTTTGCCAAAAAACAACTGGAGATGACCAACTACTCC
GTTACAGATATTGCCTACGAGTCGGGCTATAGTAGTCCAAGTCTGTTTATTAAAACCTTTAAAAAACTGACATCATTTAC
CCCAAATGGCTATCGTAAAAGGCTGACTGAATTTAATCAGTAA

Upstream 100 bases:

>100_bases
CTGGTACATGCGCAGGATCATCTAATGACCTCCATGCTGGCACGTGAACTGGTCAGTGAATTAATTGAGCTTCACGAGAA
GCTGAAACCGTAAGGAGTCG

Downstream 100 bases:

>100_bases
TTGAATTGTCCATTAAATTAATTTACACGCTTGCCTTTTAATAACACTGGCAGTGCCAGGTTATTCCTGACAGACAGGCT
AAGCATAGAACACCAGTGTT

Product: DNA-binding transcriptional regulator ChbR

Products: NA

Alternate protein names: Chb operon repressor [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MMQPQISAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYYQEINGKRVLLERGDFVFIPLGSHH
QSFYEFGATRILNVGISKRFFEQHYLPLLPFCFVASQVYRANSAFMTYVETVIASLNFRDTGLEEFVELVSFYIINRLRH
YREEQVYDDIPQWLKVTVEIMHDKTQFGDHALENMVKLSGKSQEYLTRATQRYYRKTPMQMINEIRINFAKKQLEMTNYS
VTDIAYESGYSSPSLFIKTFKKLTSFTPNGYRKRLTEFNQ

Sequences:

>Translated_280_residues
MMQPQISAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYYQEINGKRVLLERGDFVFIPLGSHH
QSFYEFGATRILNVGISKRFFEQHYLPLLPFCFVASQVYRANSAFMTYVETVIASLNFRDTGLEEFVELVSFYIINRLRH
YREEQVYDDIPQWLKVTVEIMHDKTQFGDHALENMVKLSGKSQEYLTRATQRYYRKTPMQMINEIRINFAKKQLEMTNYS
VTDIAYESGYSSPSLFIKTFKKLTSFTPNGYRKRLTEFNQ
>Mature_280_residues
MMQPQISAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYYQEINGKRVLLERGDFVFIPLGSHH
QSFYEFGATRILNVGISKRFFEQHYLPLLPFCFVASQVYRANSAFMTYVETVIASLNFRDTGLEEFVELVSFYIINRLRH
YREEQVYDDIPQWLKVTVEIMHDKTQFGDHALENMVKLSGKSQEYLTRATQRYYRKTPMQMINEIRINFAKKQLEMTNYS
VTDIAYESGYSSPSLFIKTFKKLTSFTPNGYRKRLTEFNQ

Specific function: Dual-function repressor/activator of the chbBCARFG operon. In the absence of the inducing sugar chitobiose, together with NagC, represses the chbBCARFG operon for the uptake and metabolism of chitobiose. In association with Crp, and probably in the presen

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788030, Length=280, Percent_Identity=89.6428571428572, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33098; Mature: 33098

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQPQISAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYYQEIN
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHCCCHHCCCEEEEEEEECHHHHHCC
GKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQHYLPLLPFCFVASQVYR
CCEEEEECCCEEEEECCCCHHHHHHHHHHHEECCCHHHHHHHHHCHHHHHHHHHHHHHHH
ANSAFMTYVETVIASLNFRDTGLEEFVELVSFYIINRLRHYREEQVYDDIPQWLKVTVEI
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHDKTQFGDHALENMVKLSGKSQEYLTRATQRYYRKTPMQMINEIRINFAKKQLEMTNYS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
VTDIAYESGYSSPSLFIKTFKKLTSFTPNGYRKRLTEFNQ
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MMQPQISAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYYQEIN
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHCCCHHCCCEEEEEEEECHHHHHCC
GKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQHYLPLLPFCFVASQVYR
CCEEEEECCCEEEEECCCCHHHHHHHHHHHEECCCHHHHHHHHHCHHHHHHHHHHHHHHH
ANSAFMTYVETVIASLNFRDTGLEEFVELVSFYIINRLRHYREEQVYDDIPQWLKVTVEI
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHDKTQFGDHALENMVKLSGKSQEYLTRATQRYYRKTPMQMINEIRINFAKKQLEMTNYS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
VTDIAYESGYSSPSLFIKTFKKLTSFTPNGYRKRLTEFNQ
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 9405618 [H]