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The map label for this gene is cspD [H]

Identifier: 218548403

GI number: 218548403

Start: 1056531

End: 1056752

Strand: Reverse

Name: cspD [H]

Synonym: EFER_1028

Alternate gene names: 218548403

Gene position: 1056752-1056531 (Counterclockwise)

Preceding gene: 218548406

Following gene: 218548400

Centisome position: 23.03

GC content: 43.69

Gene sequence:

>222_bases
ATGGAAAAGGGTACTGTTAAGTGGTTCAACAATGCAAAAGGGTTCGGTTTTATTTGCCCTGAAGGTGGAGGCGAAGATAT
TTTCGCGCATTACTCCACAATAAAAATGGATGGTTACAGAACGTTAAAAGCTGGCCAACCAGTCCAGTTTGATGTTACTC
AGGGACCAAAAGGCAATCATGCCAGCGTCATTGTACCCTATGAAGCTGAAGCCATTGCATAG

Upstream 100 bases:

>100_bases
ATTGTATCACGAGAGCTGGCGAGGTTTTGCACATCCCCCTCCACTCCACCACTGATTCATTCCATCTTACCTGTATAAGA
TTTACGAAGGATGTCGAAGC

Downstream 100 bases:

>100_bases
GTGATCAGTTTCATTGTGTACATCACTCAAACAAAATGCCAGCCCCATCGGCTGGCATTTTTATTTCTGTTTCTTGCATA
CTCTAAGTAATTCGCGTTGC

Product: cold shock protein-like protein

Products: NA

Alternate protein names: CSP-D [H]

Number of amino acids: Translated: 73; Mature: 73

Protein sequence:

>73_residues
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIKMDGYRTLKAGQPVQFDVTQGPKGNHASVIVPYEAEAIA

Sequences:

>Translated_73_residues
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIKMDGYRTLKAGQPVQFDVTQGPKGNHASVIVPYEAEAIA
>Mature_73_residues
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIKMDGYRTLKAGQPVQFDVTQGPKGNHASVIVPYEAEAIA

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=71, Percent_Identity=92.9577464788732, Blast_Score=140, Evalue=2e-35,
Organism=Escherichia coli, GI1786841, Length=63, Percent_Identity=52.3809523809524, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI1788126, Length=56, Percent_Identity=53.5714285714286, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI1789979, Length=55, Percent_Identity=54.5454545454545, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=51.6129032258064, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=46.7741935483871, Blast_Score=69, Evalue=5e-14,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=48.3870967741936, Blast_Score=68, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7922; Mature: 7922

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIKMDGYRTLKAGQPVQFDVTQGPKGNH
CCCCCEEEECCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCCEEEEECCCCCCCC
ASVIVPYEAEAIA
CEEEEECCCEECC
>Mature Secondary Structure
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIKMDGYRTLKAGQPVQFDVTQGPKGNH
CCCCCEEEECCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCCEEEEECCCCCCCC
ASVIVPYEAEAIA
CEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]