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The map label for this gene is ybiY [H]

Identifier: 218548344

GI number: 218548344

Start: 995653

End: 996579

Strand: Reverse

Name: ybiY [H]

Synonym: EFER_0966

Alternate gene names: 218548344

Gene position: 996579-995653 (Counterclockwise)

Preceding gene: 218548346

Following gene: 218548343

Centisome position: 21.72

GC content: 53.83

Gene sequence:

>927_bases
ATGTTTGAACGAAACAGAGAGGCAACTATGATTTTCAATATTCAGCGCTACTCGACCCATGACGGCCCCGGTATCCGCAC
GGTCGTTTTTCTTAAAGGCTGTTCACTGGGCTGCCGCTGGTGTCAGAACCCGGAAAGCCGCGCCCGCACGCAGGATCTTC
TGTATGACGCACGTCTGTGTCTGGAAGGCTGCGAGCTGTGCGCTAAGACCGCGCCGGAAGTGATTGAGCGCGCGCTGAAT
GGTTTGCTTATTCATCGGGAAAAGTTAACCCCGGAGCATCTGACGGCGTTAACCGACTGCTGCCCGACACAGGCGTTAAC
TGTGTGTGGTGAAGTGAAAAGCGTAGAGGAGATCATGGCGACTGTTCTGCGCGACAAACCGTTTTACGATCGCAGCGGCG
GCGGCTTAACGCTTTCCGGTGGTGAGCCGTTTATGCAGCCGGAAATGGCGATGGGGCTACTGCAAGCCAGCCACGAAGCC
GGTATTCATACTGCGGTAGAAACCTGTCTGCATGTGCCGTGGAAATACATCGCCCCTTCTCTGCCCTATATCGATCTGTT
TCTTGCCGATTTAAAACACGTTGCCGACGTGCCGTTTAAACAGTGGACCGACGGTAACGCCGCCAGAGTGCTGGATAACC
TGAAAAAACTCGCTGCGGCGGGGAAAAAAATCATCATCCGCGTGCCGTTGATTCAGGGCTTTAATGCCGATGAAACCTCT
GTGAAAGCCATTACTGATTTTGCCGCCGACGAGCTGCACGTCGGCGAAATTCATTTCCTGCCCTACCACACGCTGGGCAT
CAACAAATATCACTTACTTAATCTGCCCTATGACGCCCCGGAAAAACCGCTTGATGCGCCAGAACTGCTCGACTTTGCCC
AGCAGTATGCCTGCCAGAAAGGGTTAACCGCGACCTTACGAGGATAA

Upstream 100 bases:

>100_bases
CATCCTCAAAATGTACTTTTTGTCTGAATGTAGACTATCACGGGAAAACTCTCTGAACAGTTGACGCACATCAATCTAAC
TTTCATTCGAAAGTATTTTA

Downstream 100 bases:

>100_bases
CAACCATGACCACACTGAAACTGGACACGCTCAGCGACCGCATTAAAGCGCACAAAAATGCGCTGGTGAATATTGTGAAA
CCGCCGGTCTGTACCGAGCG

Product: AdoMet-dependent glycyl radical activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 3; PFL-activating enzyme 3 [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKTAPEVIERALN
GLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMGLLQASHEA
GIHTAVETCLHVPWKYIAPSLPYIDLFLADLKHVADVPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Sequences:

>Translated_308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKTAPEVIERALN
GLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMGLLQASHEA
GIHTAVETCLHVPWKYIAPSLPYIDLFLADLKHVADVPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
>Mature_308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKTAPEVIERALN
GLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMGLLQASHEA
GIHTAVETCLHVPWKYIAPSLPYIDLFLADLKHVADVPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=98.6622073578595, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1790389, Length=308, Percent_Identity=35.3896103896104, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1787130, Length=171, Percent_Identity=32.7485380116959, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790839, Length=236, Percent_Identity=25, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 34188; Mature: 34188

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLC
CCCCCCCEEEEEEEEEECCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHH
LEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMA
HHHHHHHHHCHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMGLLQASHEAGIHTAVETCLHVPWKYIAPS
HHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCC
LPYIDLFLADLKHVADVPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
CCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHH
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQK
HHHHHHHHCCCEEECEEEEECEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATLRG
CCEEECCC
>Mature Secondary Structure
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLC
CCCCCCCEEEEEEEEEECCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHH
LEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMA
HHHHHHHHHCHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMGLLQASHEAGIHTAVETCLHVPWKYIAPS
HHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCC
LPYIDLFLADLKHVADVPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
CCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHH
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQK
HHHHHHHHCCCEEECEEEEECEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATLRG
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]