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The map label for this gene is gntR [H]

Identifier: 218548247

GI number: 218548247

Start: 879387

End: 880406

Strand: Reverse

Name: gntR [H]

Synonym: EFER_0865

Alternate gene names: 218548247

Gene position: 880406-879387 (Counterclockwise)

Preceding gene: 218548253

Following gene: 218548243

Centisome position: 19.19

GC content: 48.82

Gene sequence:

>1020_bases
ATGGCAATAACCCGAAAACGTCGTAGTACAGGAAAGGTCACCATCGCCGATGTTGCGCAACTGGCTGGTGTCGGAACCAT
GACCGTATCCAGGGCGTTACGTACGCCTGAGCAAGTTTCCGATAAGCTGCGGGAGAAAATTGAAGCCGCTGTGCATGAGC
TGGGCTATATGCCCAATCTCACCGCAAGTGCACTGGCATCGGCTTCTTCACATACTATTGCGATGGTCGTTCCAAATCTG
GCTGAAGCGGGATGTTCAGAAATGTTTGCCGGACTCCAGCAAGCGCTGCAGCCTGCGGGATACCAGATCATGTTGGCAGA
ATCTCGTCACCGGTTAGATCAAGAAGAGAAACTACTGGAAACACTTCTCGCATCCAATATTGCAGCCGCTATTTTGCTTA
GTGTCGAACATAGTGACACTGTGCGTAACTGGCTCAAAAATGCCTCAATTCCGGTGATGGAGATTGGCGCGATTCGTGCC
GATCCCATTGATATGAATATTGGGATTGATAACGTAGCAGCTATGTTCGAGCTGACCGATATGCTGGTGCAGCGTGGTTA
TCAGAATATTGGCCTGTTGTGTGCTAATCAGGAACAATGGATTTTCCAACAACATTTACAAGGATGGTATAAAGCCATGC
TGCGCCATCATATGTCTCCTGATCGCGTCATCAATGCTGCTTTGCCTCCTGTGTTTTCAACTGGCGCATCACAACTCCCT
GAATTTTTACTTGCATGGCCAGAACTCGATGCTCTGGTATGCGTGTCTGATGAGCTGGCTTGCGGTGTTCTTTATGAGTG
TCAAAGAAGGCGTATCAAAGTACCAGATGAACTCGCCGTGGTCGGATTTGGTGACAGTGATGTGAGTCGGGTATGCCAAC
CACCATTAACCACTATGGCCGTTCCACATCGCAAAATTGGTTTCGAAGCAGGAAGAGCACTTCTGGAAAGGATTAAAGAC
GGCAACTGGAGCGATCGTCAATTGATCGCTCCCAGTTTATGTTTGCGAGAAAGCTGCTGA

Upstream 100 bases:

>100_bases
AACTTCTGGCTGAAATTAAGAGGTATACTGCTAGCAACAGGGAGCGGCAGATGACAACTAAAAAAGTATCTCCATTTGTT
GTAATTGAGCAGGATTTTGT

Downstream 100 bases:

>100_bases
CTTATTTTTCAGCTTCTTCCTGCTTTTCCTGAATTGCTACATCACTTCTGGCGTTGCGGGTCATCCACAGCGCCAGCGCT
TTCAGCGAATCAGGCGTAAA

Product: transcriptional regulator with PLP domain

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MAITRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNLTASALASASSHTIAMVVPNL
AEAGCSEMFAGLQQALQPAGYQIMLAESRHRLDQEEKLLETLLASNIAAAILLSVEHSDTVRNWLKNASIPVMEIGAIRA
DPIDMNIGIDNVAAMFELTDMLVQRGYQNIGLLCANQEQWIFQQHLQGWYKAMLRHHMSPDRVINAALPPVFSTGASQLP
EFLLAWPELDALVCVSDELACGVLYECQRRRIKVPDELAVVGFGDSDVSRVCQPPLTTMAVPHRKIGFEAGRALLERIKD
GNWSDRQLIAPSLCLRESC

Sequences:

>Translated_339_residues
MAITRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNLTASALASASSHTIAMVVPNL
AEAGCSEMFAGLQQALQPAGYQIMLAESRHRLDQEEKLLETLLASNIAAAILLSVEHSDTVRNWLKNASIPVMEIGAIRA
DPIDMNIGIDNVAAMFELTDMLVQRGYQNIGLLCANQEQWIFQQHLQGWYKAMLRHHMSPDRVINAALPPVFSTGASQLP
EFLLAWPELDALVCVSDELACGVLYECQRRRIKVPDELAVVGFGDSDVSRVCQPPLTTMAVPHRKIGFEAGRALLERIKD
GNWSDRQLIAPSLCLRESC
>Mature_338_residues
AITRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNLTASALASASSHTIAMVVPNLA
EAGCSEMFAGLQQALQPAGYQIMLAESRHRLDQEEKLLETLLASNIAAAILLSVEHSDTVRNWLKNASIPVMEIGAIRAD
PIDMNIGIDNVAAMFELTDMLVQRGYQNIGLLCANQEQWIFQQHLQGWYKAMLRHHMSPDRVINAALPPVFSTGASQLPE
FLLAWPELDALVCVSDELACGVLYECQRRRIKVPDELAVVGFGDSDVSRVCQPPLTTMAVPHRKIGFEAGRALLERIKDG
NWSDRQLIAPSLCLRESC

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=34.6031746031746, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=29.3929712460064, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1790369, Length=327, Percent_Identity=29.0519877675841, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=28.6206896551724, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788474, Length=292, Percent_Identity=29.7945205479452, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=25.748502994012, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=26.4437689969605, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787580, Length=333, Percent_Identity=25.2252252252252, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787906, Length=335, Percent_Identity=25.9701492537313, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786540, Length=331, Percent_Identity=26.2839879154079, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=25.297619047619, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=24.3076923076923, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1786268, Length=303, Percent_Identity=28.0528052805281, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37253; Mature: 37121

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNL
CCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
TASALASASSHTIAMVVPNLAEAGCSEMFAGLQQALQPAGYQIMLAESRHRLDQEEKLLE
HHHHHHCCCCCEEEEEECCHHHCCHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHH
TLLASNIAAAILLSVEHSDTVRNWLKNASIPVMEIGAIRADPIDMNIGIDNVAAMFELTD
HHHHHHHHHHHHEECCCHHHHHHHHHCCCCCEEEECCEECCCCEECCCHHHHHHHHHHHH
MLVQRGYQNIGLLCANQEQWIFQQHLQGWYKAMLRHHMSPDRVINAALPPVFSTGASQLP
HHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCHHHHH
EFLLAWPELDALVCVSDELACGVLYECQRRRIKVPDELAVVGFGDSDVSRVCQPPLTTMA
HHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCCHHHHC
VPHRKIGFEAGRALLERIKDGNWSDRQLIAPSLCLRESC
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AITRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLREKIEAAVHELGYMPNL
CCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
TASALASASSHTIAMVVPNLAEAGCSEMFAGLQQALQPAGYQIMLAESRHRLDQEEKLLE
HHHHHHCCCCCEEEEEECCHHHCCHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHH
TLLASNIAAAILLSVEHSDTVRNWLKNASIPVMEIGAIRADPIDMNIGIDNVAAMFELTD
HHHHHHHHHHHHEECCCHHHHHHHHHCCCCCEEEECCEECCCCEECCCHHHHHHHHHHHH
MLVQRGYQNIGLLCANQEQWIFQQHLQGWYKAMLRHHMSPDRVINAALPPVFSTGASQLP
HHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCHHHHH
EFLLAWPELDALVCVSDELACGVLYECQRRRIKVPDELAVVGFGDSDVSRVCQPPLTTMA
HHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCCHHHHC
VPHRKIGFEAGRALLERIKDGNWSDRQLIAPSLCLRESC
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]