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The map label for this gene is purN [H]

Identifier: 218548067

GI number: 218548067

Start: 692406

End: 693047

Strand: Reverse

Name: purN [H]

Synonym: EFER_0676

Alternate gene names: 218548067

Gene position: 693047-692406 (Counterclockwise)

Preceding gene: 218548068

Following gene: 218548066

Centisome position: 15.1

GC content: 48.6

Gene sequence:

>642_bases
TTGATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGCAATTTGCAGGCAATCATCGATGCCTGCAAAGTCAATAAAAT
CAAAGGCACCGTACGGGCAGTCTTCAGCAATAAGGCCGACGCGTTCGGCCTTGAACGTGCCAGGGAAGCGGGTATCCCAA
CGCATGTATTGTCAGCGAATGATTTTGCTAACCGCGACGCTTTTGACAAGCAACTTATTGCTGAGATTGACAACTACACG
CCCGATGTTGTGGTGCTGGCGGGTTTTATGCGTATTTTAAGCCCGACATTTGTGGAGCATTACGCAGAAAAGTTGCTGAA
TATTCATCCCTCTTTGCTACCTAAATATCCAGGGCTTCATACCCATCGCCAGGCTCTGGAAAATGGTGATAAAGAGCATG
GAACATCCGTGCATTTTGTCACAGATGAACTGGACGGTGGCCCGGTAATATTACAGGCCAGAGTGCCGGTGTTTGCAGGT
GATACTGAAGATGATGTGACGGCTCGTGTGCAAACGCAGGAACATGCCATTTATCCTCTGGTCATTAGTTGGTTTGTTGA
TGGTCGGTTGAAAATGCGCGATAACGCCGCCTGGCTGGATGGTAAACGTCTGCCTCCTCAAGGCTATGCGGCTGACGAAT
AA

Upstream 100 bases:

>100_bases
AAGTCGAAAACGCCCTCGCCCTGCTCAATGCCAACGGTGAAAAGGCCTGGAAAATTGGGGTGATTAAAGCCTCTGATGCC
GAACAACGTGTGGTTATTGC

Downstream 100 bases:

>100_bases
CTATTTTTTTCTGTTCCTCACGGTTCTGCTTGCGTGAGGAACGCTCTTCCCCGCCGTGTTCTCTACGTCATTTAACAGCA
TTTAAAATCGGTTCTCCACC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MMNIVVLISGNGSNLQAIIDACKVNKIKGTVRAVFSNKADAFGLERAREAGIPTHVLSANDFANRDAFDKQLIAEIDNYT
PDVVVLAGFMRILSPTFVEHYAEKLLNIHPSLLPKYPGLHTHRQALENGDKEHGTSVHFVTDELDGGPVILQARVPVFAG
DTEDDVTARVQTQEHAIYPLVISWFVDGRLKMRDNAAWLDGKRLPPQGYAADE

Sequences:

>Translated_213_residues
MMNIVVLISGNGSNLQAIIDACKVNKIKGTVRAVFSNKADAFGLERAREAGIPTHVLSANDFANRDAFDKQLIAEIDNYT
PDVVVLAGFMRILSPTFVEHYAEKLLNIHPSLLPKYPGLHTHRQALENGDKEHGTSVHFVTDELDGGPVILQARVPVFAG
DTEDDVTARVQTQEHAIYPLVISWFVDGRLKMRDNAAWLDGKRLPPQGYAADE
>Mature_213_residues
MMNIVVLISGNGSNLQAIIDACKVNKIKGTVRAVFSNKADAFGLERAREAGIPTHVLSANDFANRDAFDKQLIAEIDNYT
PDVVVLAGFMRILSPTFVEHYAEKLLNIHPSLLPKYPGLHTHRQALENGDKEHGTSVHFVTDELDGGPVILQARVPVFAG
DTEDDVTARVQTQEHAIYPLVISWFVDGRLKMRDNAAWLDGKRLPPQGYAADE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=43.4343434343434, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=43.4343434343434, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=43.4343434343434, Blast_Score=171, Evalue=6e-43,
Organism=Escherichia coli, GI1788846, Length=212, Percent_Identity=86.7924528301887, Blast_Score=389, Evalue=1e-110,
Organism=Escherichia coli, GI1787483, Length=163, Percent_Identity=33.1288343558282, Blast_Score=88, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=178, Percent_Identity=38.2022471910112, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=32.6633165829146, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=48.6631016042781, Blast_Score=187, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23485; Mature: 23485

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNIVVLISGNGSNLQAIIDACKVNKIKGTVRAVFSNKADAFGLERAREAGIPTHVLSAN
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCC
DFANRDAFDKQLIAEIDNYTPDVVVLAGFMRILSPTFVEHYAEKLLNIHPSLLPKYPGLH
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHCCCCCCCH
THRQALENGDKEHGTSVHFVTDELDGGPVILQARVPVFAGDTEDDVTARVQTQEHAIYPL
HHHHHHHCCCCHHCCEEEEEEECCCCCCEEEEEECCEEECCCCCCCEEEEECCCCCHHHH
VISWFVDGRLKMRDNAAWLDGKRLPPQGYAADE
HEEEECCCEEEEECCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MMNIVVLISGNGSNLQAIIDACKVNKIKGTVRAVFSNKADAFGLERAREAGIPTHVLSAN
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCC
DFANRDAFDKQLIAEIDNYTPDVVVLAGFMRILSPTFVEHYAEKLLNIHPSLLPKYPGLH
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHCCCCCCCH
THRQALENGDKEHGTSVHFVTDELDGGPVILQARVPVFAGDTEDDVTARVQTQEHAIYPL
HHHHHHHCCCCHHCCEEEEEEECCCCCCEEEEEECCEEECCCCCCCEEEEECCCCCHHHH
VISWFVDGRLKMRDNAAWLDGKRLPPQGYAADE
HEEEECCCEEEEECCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]