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The map label for this gene is sseA [H]

Identifier: 218548042

GI number: 218548042

Start: 649431

End: 650276

Strand: Reverse

Name: sseA [H]

Synonym: EFER_0651

Alternate gene names: 218548042

Gene position: 650276-649431 (Counterclockwise)

Preceding gene: 218548057

Following gene: 218548030

Centisome position: 14.17

GC content: 51.06

Gene sequence:

>846_bases
ATGACCACAACCTGGTTTGTTGCAGCCGACTGGCTCGCCGAGCATATTAACGATCCAGAAATTCAAATTATTGATGCCCG
TATGGCTCCACCTGGAGAGAAGGAGCGTCATGTTTTACAGGAGTACCGCGAAGGACATATTCCTGGTGCCGTTTTTTTCG
ATATTGAAGCCCTTTCTGATCACACGTCCTCCTTTCCACATATGATGCCGCGCCCGGAAGCGTTTGCCGTCGCTATGCGT
GAACTGGGCGTTAATCAGGATAAGCATCTGATTGTATACGATGAAGGTAATCTCTTTTCTGCGCCACGCGCATGGTGGAT
GCTGCGCACTTTTGGTGTCGAAAGAGTATCGATTCTCGCTGGCGGATTAACAGGATGGCAACGCGATGAGTTACTTCTGG
AAAAAGGAGATGTGGAACTACCTGAAGGCGAGTTTACAGCAAAATTCACCCCGGATGCGGTAGTAAATGTCACGGACGTT
CTGTTGGCAAGCCATGAAAAAACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAATGCAGAAGTTGATGAACC
TCGCGCAGGTTTACGTCGCGGACATATTCCCGGTGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTGA
AAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCT
GGTGTAACGGCGGCCGTGGTTTTGTTGGCACTCGCGACACTCGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAG
TGAATGGGGCGCACGAGCAGATTTACCGGTTGAGCCAGTGAAATAA

Upstream 100 bases:

>100_bases
GCGAAATGTGGCTTAAGCAGCCAAATTAATCCCTTTTGCTTGTCGCTTTACGCTAAACCACCGACAATTTATCAGTAACC
CGGCAACAATGGAGATGTCT

Downstream 100 bases:

>100_bases
GCTTTATACAGGCAATAAAAAACCGCCAAATTTGGCGGTTTTTTATTGCTGGTCTGGTTCGCGGCCTTTCCAGCAGGTTG
TATTACTGCAAGTGTATCTC

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTTTWFVAADWLAEHINDPEIQIIDARMAPPGEKERHVLQEYREGHIPGAVFFDIEALSDHTSSFPHMMPRPEAFAVAMR
ELGVNQDKHLIVYDEGNLFSAPRAWWMLRTFGVERVSILAGGLTGWQRDELLLEKGDVELPEGEFTAKFTPDAVVNVTDV
LLASHEKTAQIIDARPAARFNAEVDEPRAGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFGRGVSYDKPIIVSCGS
GVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK

Sequences:

>Translated_281_residues
MTTTWFVAADWLAEHINDPEIQIIDARMAPPGEKERHVLQEYREGHIPGAVFFDIEALSDHTSSFPHMMPRPEAFAVAMR
ELGVNQDKHLIVYDEGNLFSAPRAWWMLRTFGVERVSILAGGLTGWQRDELLLEKGDVELPEGEFTAKFTPDAVVNVTDV
LLASHEKTAQIIDARPAARFNAEVDEPRAGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFGRGVSYDKPIIVSCGS
GVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK
>Mature_280_residues
TTTWFVAADWLAEHINDPEIQIIDARMAPPGEKERHVLQEYREGHIPGAVFFDIEALSDHTSSFPHMMPRPEAFAVAMRE
LGVNQDKHLIVYDEGNLFSAPRAWWMLRTFGVERVSILAGGLTGWQRDELLLEKGDVELPEGEFTAKFTPDAVVNVTDVL
LASHEKTAQIIDARPAARFNAEVDEPRAGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFGRGVSYDKPIIVSCGSG
VTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=275, Percent_Identity=40, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI61835204, Length=275, Percent_Identity=40, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI194473681, Length=275, Percent_Identity=40, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI17402865, Length=276, Percent_Identity=37.6811594202899, Blast_Score=172, Evalue=3e-43,
Organism=Escherichia coli, GI87082121, Length=281, Percent_Identity=90.0355871886121, Blast_Score=519, Evalue=1e-149,
Organism=Escherichia coli, GI87081967, Length=254, Percent_Identity=23.2283464566929, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI115534702, Length=212, Percent_Identity=32.0754716981132, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71997283, Length=299, Percent_Identity=23.7458193979933, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17561888, Length=268, Percent_Identity=26.4925373134328, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543836, Length=241, Percent_Identity=24.896265560166, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543838, Length=241, Percent_Identity=24.896265560166, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559150, Length=251, Percent_Identity=26.2948207171315, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324825, Length=266, Percent_Identity=33.8345864661654, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31119; Mature: 30987

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTWFVAADWLAEHINDPEIQIIDARMAPPGEKERHVLQEYREGHIPGAVFFDIEALSD
CCEEEEEHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHC
HTSSFPHMMPRPEAFAVAMRELGVNQDKHLIVYDEGNLFSAPRAWWMLRTFGVERVSILA
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEECCCHHHHHHHHHCCCEEEEEE
GGLTGWQRDELLLEKGDVELPEGEFTAKFTPDAVVNVTDVLLASHEKTAQIIDARPAARF
CCCCCCCCCHHHEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHCCCCCHHC
NAEVDEPRAGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFGRGVSYDKPIIVSCGS
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEECCC
GVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK
CHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TTTWFVAADWLAEHINDPEIQIIDARMAPPGEKERHVLQEYREGHIPGAVFFDIEALSD
CEEEEEHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHC
HTSSFPHMMPRPEAFAVAMRELGVNQDKHLIVYDEGNLFSAPRAWWMLRTFGVERVSILA
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEECCCHHHHHHHHHCCCEEEEEE
GGLTGWQRDELLLEKGDVELPEGEFTAKFTPDAVVNVTDVLLASHEKTAQIIDARPAARF
CCCCCCCCCHHHEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHCCCCCHHC
NAEVDEPRAGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFGRGVSYDKPIIVSCGS
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEECCC
GVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK
CHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]