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The map label for this gene is aes [H]

Identifier: 218547923

GI number: 218547923

Start: 526474

End: 527490

Strand: Reverse

Name: aes [H]

Synonym: EFER_0526

Alternate gene names: 218547923

Gene position: 527490-526474 (Counterclockwise)

Preceding gene: 218547927

Following gene: 218547915

Centisome position: 11.5

GC content: 50.84

Gene sequence:

>1017_bases
TTGCTAGTGGCGGTTTATTACAATGACGTAATTTGGAAGGAGTTTTTTATGAAACCGGAAAACAAATTACCCGTTCTGGA
GCTCATTTCTGCTGAAATGAAGGCAGTTGTGAATACTTTTCAGGCGGATTTGCCGCCCTGGCCCGCAACGGGAACGATTG
CAGAGCAACGTCAGTATTACACGCTTGAGCGCCGACTCTGGAATGCGGGCGCTCCAGAAATGGCAACCAGCGCTTACATG
GTTCCCACACCTTATGGTCAGGTCGAGACTCGCCTCTACTCACCCAAAAGTCACAGCCCGGCGACACTATTTTATCTGCA
TGGTGGTGGTTTTATTCTCGGCAATCTTGATACCCACGATCGGATTATGCGACTGCTGGCAAGTTACACCCAATGTACAG
TGATTGGTATTGCTTACACTCTTTCGCCGGAAGCACGTTTTCCGCAGGCAATAGAGGAAATTGTGGCTGCCTGTTGCCAC
TTCCACCAGCAGGCAGAGGATTATCAAATCAATATGTCACGCATTGGTTTTGCCGGTGATTCTGCAGGCGCAATGCTGGC
GCTCGCCAGTGCGTTGTGGTTGCGTGATAAACAGATAGATTGCGGTGAAGTTGCGGGCGTTTTGCTGTGGTATGGGCTTT
ACGGATTACGGGATTCCGTGACTCGTCGTCTGTTGGGCGGTGTTTGGGATGGCTTAACGCAAGAGGATTTACAGATGTAT
GAAGAGGCGTATTTAAGCCACGACACAGACCGCGAGTCGCCGTATTACTGCCTGTTTAATAATGATCTCACCCGCGAAGT
ACCGCCCTGCTTTATCGCTGGGGCAGAGTTCGACCCTCTGCTGGATGACAGCCGTCTGCTTTACCAGACGTTAGCGGCAC
ATCAGCAGCCTTGTAAGTTCAAACTCTACCCAGGCACGCTGCACGCCTTTTTGCATTATTCACGGATGATGAAAACCGCC
GACGATGCTCTTCGCGACGGCGCTCAGTTCTTTAGCGATACGCTGCAACAAGACTAG

Upstream 100 bases:

>100_bases
TGTTTTTACGGGTGGTTAACTGAGCGGCGAATGTTAGCACATTCACACCTGAACGCGCGATACACACTCGGCTTTCAATA
TCGGACGGCGGGATCGCATT

Downstream 100 bases:

>100_bases
CCATCATTTCGATATGTTCCGGTGCGGCATTAAGCGCCGGAATATATTCGTATTTTTTCCCGCCCGCACCGAGGAAGACT
TCACGGTTTTGCTCGGCAAT

Product: acetyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MLVAVYYNDVIWKEFFMKPENKLPVLELISAEMKAVVNTFQADLPPWPATGTIAEQRQYYTLERRLWNAGAPEMATSAYM
VPTPYGQVETRLYSPKSHSPATLFYLHGGGFILGNLDTHDRIMRLLASYTQCTVIGIAYTLSPEARFPQAIEEIVAACCH
FHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRDKQIDCGEVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQEDLQMY
EEAYLSHDTDRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCKFKLYPGTLHAFLHYSRMMKTA
DDALRDGAQFFSDTLQQD

Sequences:

>Translated_338_residues
MLVAVYYNDVIWKEFFMKPENKLPVLELISAEMKAVVNTFQADLPPWPATGTIAEQRQYYTLERRLWNAGAPEMATSAYM
VPTPYGQVETRLYSPKSHSPATLFYLHGGGFILGNLDTHDRIMRLLASYTQCTVIGIAYTLSPEARFPQAIEEIVAACCH
FHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRDKQIDCGEVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQEDLQMY
EEAYLSHDTDRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCKFKLYPGTLHAFLHYSRMMKTA
DDALRDGAQFFSDTLQQD
>Mature_338_residues
MLVAVYYNDVIWKEFFMKPENKLPVLELISAEMKAVVNTFQADLPPWPATGTIAEQRQYYTLERRLWNAGAPEMATSAYM
VPTPYGQVETRLYSPKSHSPATLFYLHGGGFILGNLDTHDRIMRLLASYTQCTVIGIAYTLSPEARFPQAIEEIVAACCH
FHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRDKQIDCGEVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQEDLQMY
EEAYLSHDTDRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCKFKLYPGTLHAFLHYSRMMKTA
DDALRDGAQFFSDTLQQD

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=132, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI157041239, Length=133, Percent_Identity=27.0676691729323, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1786682, Length=319, Percent_Identity=92.4764890282132, Blast_Score=619, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI71996133, Length=267, Percent_Identity=26.5917602996255, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=141, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=130, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656084, Length=100, Percent_Identity=37, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24656076, Length=100, Percent_Identity=37, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20130169, Length=100, Percent_Identity=37, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 38282; Mature: 38282

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVAVYYNDVIWKEFFMKPENKLPVLELISAEMKAVVNTFQADLPPWPATGTIAEQRQYY
CEEEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TLERRLWNAGAPEMATSAYMVPTPYGQVETRLYSPKSHSPATLFYLHGGGFILGNLDTHD
HHHHHHHCCCCCCHHCCEEECCCCCCCHHHEEECCCCCCCEEEEEEECCEEEEECCCHHH
RIMRLLASYTQCTVIGIAYTLSPEARFPQAIEEIVAACCHFHQQAEDYQINMSRIGFAGD
HHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCC
SAGAMLALASALWLRDKQIDCGEVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQEDLQMY
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EEAYLSHDTDRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCKF
HHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEE
KLYPGTLHAFLHYSRMMKTADDALRDGAQFFSDTLQQD
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLVAVYYNDVIWKEFFMKPENKLPVLELISAEMKAVVNTFQADLPPWPATGTIAEQRQYY
CEEEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TLERRLWNAGAPEMATSAYMVPTPYGQVETRLYSPKSHSPATLFYLHGGGFILGNLDTHD
HHHHHHHCCCCCCHHCCEEECCCCCCCHHHEEECCCCCCCEEEEEEECCEEEEECCCHHH
RIMRLLASYTQCTVIGIAYTLSPEARFPQAIEEIVAACCHFHQQAEDYQINMSRIGFAGD
HHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCC
SAGAMLALASALWLRDKQIDCGEVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQEDLQMY
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EEAYLSHDTDRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCKF
HHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEE
KLYPGTLHAFLHYSRMMKTADDALRDGAQFFSDTLQQD
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA