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The map label for this gene is ahpF [H]

Identifier: 218235324

GI number: 218235324

Start: 367586

End: 369112

Strand: Reverse

Name: ahpF [H]

Synonym: BCB4264_A0391

Alternate gene names: 218235324

Gene position: 369112-367586 (Counterclockwise)

Preceding gene: 218234783

Following gene: 218233134

Centisome position: 6.81

GC content: 40.8

Gene sequence:

>1527_bases
ATGATACTAGATGCAGATATAAAAACACAACTATCCCAATACCTTCAATTAATGGAGAACGATATTTTACTTAAAGTAAG
CGCAGGAGACGATAACGTATCTAAAGATATGTTAGCTCTAGTAGACGAATTAGCTACTATGTCATCTAAGATTACAGTAG
AAAAAGTTGAATTAGAGAGAACACCAAGCTTTAGCGTAAACCGCCCTGGTGAAAACACTGGTGTCGTATTCGCTGGTATT
CCATTAGGACACGAATTTACATCATTAGTGTTAGCTTTACTACAAGTAAGTGGACGCGCTCCAAAAGTTGAACAAAAATT
AATCGATCAAATTAAAAACATTCAAGGCGAATATCATTTTGAATCTTATATCAGCCTAAGTTGTCATAACTGTCCTGATG
TTGTACAAGCTCTTAACGTAATGAGCGTTCTTAACCCTGGTATTACACATACTATGATTGATGGCGCTGCATTCAAAGAG
GAAGTAGAAAGCAAAGACATTATGGCAGTACCAACTGTTTACCTAAACGGCGAATCTTTCGGAAGCGGTCGTATGACACT
TGAAGAAATTTTAGCTAAAATGGGTAACGGTCCAGATGCATCAGAGCTTTCTGATAAAGATCCATACGATGTTCTTGTTG
TTGGTGGCGGCCCTGCTGGTGCAAGTGCAGCAATTTACGCAGCACGTAAAGGCATCCGCACTGGTATCGTTGCTGAGCGC
TTCGGTGGTCAAGTAATGGATACTATGGGCATTGAGAACTTCATCAGCGTGAAAAAGACTGAAGGTCCTAAGCTAGTAGC
AAGCCTTGAAGAGCACGTAAAAGAATACGACATCGATGTAATGAATCTACAACGTGCGAAACGTTTAGAGAAAAAAGAAC
TTATTGAAGTGGAACTTGAAAACGGCGCTATTCTGAAAAGTAAGAGCGTAATCGTTTCTACAGGTGCTCGCTGGCGTAAT
GTTGGCGTACCAGGTGAAGCTGAGTTCAAAAATAAAGGTGTAGCATATTGCCCACACTGTGACGGTCCATTATTCACTGG
AAAAGACGTAGCAGTTATCGGCGGAGGTAACTCTGGTATTGAAGCAGCTATCGACTTAGCAGGTATCGTTAAGCACGTAA
CTGTTCTTGAATTCATGCCAGAATTAAAAGCTGATGCTGTATTACAAGAGCGTCTTAACAGCTTACCAAACGTAACTGTT
CTGAAAAACGTTCAAACGAAAGAAATCACTGGTACTGATAAAGTAAACGGTATTTCTTACATCGATCGTGAAACTGAAGA
AGTTCATCACGTTGAATTACAAGGTGTATTCGTACAAATCGGTCTTGTACCAAACACAGACTGGTTAGGCGAAACAGTTG
AACGCGTTCGCGGTGAAATCGTAACAGACAAGCACGGCGCTACAAACGTGCCAGGAGTGTTTGCTGCAGGTGACTGTACA
AATAATCCGTACAAACAAATCATCATCTCTATGGGTTCAGGTGCAAACGCAGCTTTAGGTGCATTTGATTACTTAATCCG
TAACTAA

Upstream 100 bases:

>100_bases
ACAACCCAGGTGAAGTTTGCCCAGCTAAATGGCAAGAGGGTTCTGCAACACTTAAACCAAGCCTTGACCTTGTAGGCAAA
ATCTAAGGAGTTCGATCAAA

Downstream 100 bases:

>100_bases
TAAAAAAACAGGTGCCTTGGCACCTGTTTTTTTATTACCCTAATGTATAAACTAAAAACCTCTCATTCCCATTCTTCTCA
TAAGCACCTGACAATCTAAC

Product: alkyl hydroperoxide reductase, F subunit

Products: NA

Alternate protein names: Alkyl hydroperoxide reductase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MILDADIKTQLSQYLQLMENDILLKVSAGDDNVSKDMLALVDELATMSSKITVEKVELERTPSFSVNRPGENTGVVFAGI
PLGHEFTSLVLALLQVSGRAPKVEQKLIDQIKNIQGEYHFESYISLSCHNCPDVVQALNVMSVLNPGITHTMIDGAAFKE
EVESKDIMAVPTVYLNGESFGSGRMTLEEILAKMGNGPDASELSDKDPYDVLVVGGGPAGASAAIYAARKGIRTGIVAER
FGGQVMDTMGIENFISVKKTEGPKLVASLEEHVKEYDIDVMNLQRAKRLEKKELIEVELENGAILKSKSVIVSTGARWRN
VGVPGEAEFKNKGVAYCPHCDGPLFTGKDVAVIGGGNSGIEAAIDLAGIVKHVTVLEFMPELKADAVLQERLNSLPNVTV
LKNVQTKEITGTDKVNGISYIDRETEEVHHVELQGVFVQIGLVPNTDWLGETVERVRGEIVTDKHGATNVPGVFAAGDCT
NNPYKQIIISMGSGANAALGAFDYLIRN

Sequences:

>Translated_508_residues
MILDADIKTQLSQYLQLMENDILLKVSAGDDNVSKDMLALVDELATMSSKITVEKVELERTPSFSVNRPGENTGVVFAGI
PLGHEFTSLVLALLQVSGRAPKVEQKLIDQIKNIQGEYHFESYISLSCHNCPDVVQALNVMSVLNPGITHTMIDGAAFKE
EVESKDIMAVPTVYLNGESFGSGRMTLEEILAKMGNGPDASELSDKDPYDVLVVGGGPAGASAAIYAARKGIRTGIVAER
FGGQVMDTMGIENFISVKKTEGPKLVASLEEHVKEYDIDVMNLQRAKRLEKKELIEVELENGAILKSKSVIVSTGARWRN
VGVPGEAEFKNKGVAYCPHCDGPLFTGKDVAVIGGGNSGIEAAIDLAGIVKHVTVLEFMPELKADAVLQERLNSLPNVTV
LKNVQTKEITGTDKVNGISYIDRETEEVHHVELQGVFVQIGLVPNTDWLGETVERVRGEIVTDKHGATNVPGVFAAGDCT
NNPYKQIIISMGSGANAALGAFDYLIRN
>Mature_508_residues
MILDADIKTQLSQYLQLMENDILLKVSAGDDNVSKDMLALVDELATMSSKITVEKVELERTPSFSVNRPGENTGVVFAGI
PLGHEFTSLVLALLQVSGRAPKVEQKLIDQIKNIQGEYHFESYISLSCHNCPDVVQALNVMSVLNPGITHTMIDGAAFKE
EVESKDIMAVPTVYLNGESFGSGRMTLEEILAKMGNGPDASELSDKDPYDVLVVGGGPAGASAAIYAARKGIRTGIVAER
FGGQVMDTMGIENFISVKKTEGPKLVASLEEHVKEYDIDVMNLQRAKRLEKKELIEVELENGAILKSKSVIVSTGARWRN
VGVPGEAEFKNKGVAYCPHCDGPLFTGKDVAVIGGGNSGIEAAIDLAGIVKHVTVLEFMPELKADAVLQERLNSLPNVTV
LKNVQTKEITGTDKVNGISYIDRETEEVHHVELQGVFVQIGLVPNTDWLGETVERVRGEIVTDKHGATNVPGVFAAGDCT
NNPYKQIIISMGSGANAALGAFDYLIRN

Specific function: Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=517, Percent_Identity=57.6402321083172, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1787114, Length=307, Percent_Identity=33.2247557003257, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321898, Length=327, Percent_Identity=32.1100917431193, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320560, Length=320, Percent_Identity=32.1875, Blast_Score=130, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.6.99.3 [H]

Molecular weight: Translated: 54808; Mature: 54808

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDADIKTQLSQYLQLMENDILLKVSAGDDNVSKDMLALVDELATMSSKITVEKVELER
CEECCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECC
TPSFSVNRPGENTGVVFAGIPLGHEFTSLVLALLQVSGRAPKVEQKLIDQIKNIQGEYHF
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEE
ESYISLSCHNCPDVVQALNVMSVLNPGITHTMIDGAAFKEEVESKDIMAVPTVYLNGESF
EEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHCCCCEEEECEEEECCCCC
GSGRMTLEEILAKMGNGPDASELSDKDPYDVLVVGGGPAGASAAIYAARKGIRTGIVAER
CCCCCHHHHHHHHHCCCCCHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHH
FGGQVMDTMGIENFISVKKTEGPKLVASLEEHVKEYDIDVMNLQRAKRLEKKELIEVELE
HCCHHHHHHCHHHHHEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCEEEEEEC
NGAILKSKSVIVSTGARWRNVGVPGEAEFKNKGVAYCPHCDGPLFTGKDVAVIGGGNSGI
CCEEEECCCEEEECCCCEEECCCCCCCCCCCCCCEECCCCCCCEECCCCEEEEECCCCCH
EAAIDLAGIVKHVTVLEFMPELKADAVLQERLNSLPNVTVLKNVQTKEITGTDKVNGISY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHH
IDRETEEVHHVELQGVFVQIGLVPNTDWLGETVERVRGEIVTDKHGATNVPGVFAAGDCT
HCCCHHHHHEEEEEEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCC
NNPYKQIIISMGSGANAALGAFDYLIRN
CCCHHEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MILDADIKTQLSQYLQLMENDILLKVSAGDDNVSKDMLALVDELATMSSKITVEKVELER
CEECCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECC
TPSFSVNRPGENTGVVFAGIPLGHEFTSLVLALLQVSGRAPKVEQKLIDQIKNIQGEYHF
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEE
ESYISLSCHNCPDVVQALNVMSVLNPGITHTMIDGAAFKEEVESKDIMAVPTVYLNGESF
EEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHCCCCEEEECEEEECCCCC
GSGRMTLEEILAKMGNGPDASELSDKDPYDVLVVGGGPAGASAAIYAARKGIRTGIVAER
CCCCCHHHHHHHHHCCCCCHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHH
FGGQVMDTMGIENFISVKKTEGPKLVASLEEHVKEYDIDVMNLQRAKRLEKKELIEVELE
HCCHHHHHHCHHHHHEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCEEEEEEC
NGAILKSKSVIVSTGARWRNVGVPGEAEFKNKGVAYCPHCDGPLFTGKDVAVIGGGNSGI
CCEEEECCCEEEECCCCEEECCCCCCCCCCCCCCEECCCCCCCEECCCCEEEEECCCCCH
EAAIDLAGIVKHVTVLEFMPELKADAVLQERLNSLPNVTVLKNVQTKEITGTDKVNGISY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHH
IDRETEEVHHVELQGVFVQIGLVPNTDWLGETVERVRGEIVTDKHGATNVPGVFAAGDCT
HCCCHHHHHEEEEEEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCC
NNPYKQIIISMGSGANAALGAFDYLIRN
CCCHHEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1917890; 2760020 [H]