Click here to switch to the map view.

The map label for this gene is kgtP [H]

Identifier: 218233373

GI number: 218233373

Start: 937203

End: 938525

Strand: Reverse

Name: kgtP [H]

Synonym: BCB4264_A0964

Alternate gene names: 218233373

Gene position: 938525-937203 (Counterclockwise)

Preceding gene: 218235139

Following gene: 218236156

Centisome position: 17.32

GC content: 43.84

Gene sequence:

>1323_bases
ATGGCTCAAGCAAAATCAAACCGAGTCGCCGGCAATATTTTCAAAGGTTCAGTCGGCAATTTAATCGAATGGTACGACTG
GTACGTTTACTCTGCTTTCGCTGTTTATTTTTCAGCGGAGTTCTTTCCTAAAGGAGATCCAACGAGTCAATTACTGAACA
CAGCAGCTATTTTCGCAGTTGGGTTTTTAATGCGCCCTATTGGGAGTTTATTAATGGGACGTTATGCGGATCGGCACGGA
CGCCGGGCAGCATTAACGCTTTCGATTACGGTTATGGCCGGTGGTTCTTTCATTATTGCCTGTACACCGAGTTACGAAAG
TATCGGTATTATGGCGCCAATTATTTTAGTTCTTGCTCGTTTACTTCAAGGGTTGTCACTCGGCGGAGAGTATGGAACTT
CCGCAACGTATTTATCTGAAATGGCTAGTAGTGGCCGCCGTGGTTTTTATTCAAGTTTCCAATATGTAACGCTCGTTGCT
GGACAGATGGTTGCGTTAGGCGTTCAAATTGTTCTTCAGCAATTACTAAGCGAACCAGATATGAAAGCTTGGGGATGGCG
CATTCCATTCATTATTGGAGCGATGGGCGCAGTAGCTGTATTATGGCTTCGCCGCACGATGGATGAGTCGGAGCAGTTCT
CAAACATAAAATCACAAAAACGCGAAAGTGCAGGAACTGTTCGCGCTCTTATGAAACACCCGAAAGCAGTATTAACGGTA
GTCGGTCTAACATTAGGAGGCACTGTTGCATTTTATACGTACACAACATATTTACAAAAGTTCATGGTAAACACAGTCGG
CCTTCCGAAAGAAGTTGTAAGCTGGATTAACTTTGTCGCACTACTTATTTTCGTCGTACTTCAACCAATTGCAGGATTAC
TATCCGATAAAATTGGACGCCGCCCACTATTAATGGCATTCGGTATTCTCGGGACATTACTAACAGCGCCCATCTTCTTC
TTCATGGAAAAAACGACAGAACCAATCGTAGCATTTTTACTCATGATGGTCGGTCTCATTATCGTTACTGGTTACACTTC
CATTAACGCAATTGTAAAAGCAGAACTCTTCCCGACCGAAATTCGCGCACTTGGCGTAGGTTTACCATATGCACTAACCG
TTGCGATATTTGGTGGGACAGCCGAGTTCATCGCATTATGGCTAAAGAGTATCGGAATGGAATCACTATTCTACTTCTAC
GTAGCTGGATGTATCGCGATCAGCTTTATTACGTATTGGCGTATGGATGAGTCATCGAAGACTTCGCAGATTGAGGCAGA
GCTTGGTGGTGGGGATACACTAGTTAATAATAAGTCTAGTTAA

Upstream 100 bases:

>100_bases
TCATAATATTGAGATTTCATTTAAAGAACAGTACAATTCAACTACAATGAACACGCTTTCATAATTATCTTTTTATTACT
AAGCAAGAGGAGGAATTAGT

Downstream 100 bases:

>100_bases
AATTATGTATTCTATGTTTTTCGTTAATCTGAACTCGATGAATCTCTTTCTTGGCAGGGTAAAGATGTTGTTGGTGCTTT
AGAGGTAAGATTAGGATTTA

Product: putative alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MAQAKSNRVAGNIFKGSVGNLIEWYDWYVYSAFAVYFSAEFFPKGDPTSQLLNTAAIFAVGFLMRPIGSLLMGRYADRHG
RRAALTLSITVMAGGSFIIACTPSYESIGIMAPIILVLARLLQGLSLGGEYGTSATYLSEMASSGRRGFYSSFQYVTLVA
GQMVALGVQIVLQQLLSEPDMKAWGWRIPFIIGAMGAVAVLWLRRTMDESEQFSNIKSQKRESAGTVRALMKHPKAVLTV
VGLTLGGTVAFYTYTTYLQKFMVNTVGLPKEVVSWINFVALLIFVVLQPIAGLLSDKIGRRPLLMAFGILGTLLTAPIFF
FMEKTTEPIVAFLLMMVGLIIVTGYTSINAIVKAELFPTEIRALGVGLPYALTVAIFGGTAEFIALWLKSIGMESLFYFY
VAGCIAISFITYWRMDESSKTSQIEAELGGGDTLVNNKSS

Sequences:

>Translated_440_residues
MAQAKSNRVAGNIFKGSVGNLIEWYDWYVYSAFAVYFSAEFFPKGDPTSQLLNTAAIFAVGFLMRPIGSLLMGRYADRHG
RRAALTLSITVMAGGSFIIACTPSYESIGIMAPIILVLARLLQGLSLGGEYGTSATYLSEMASSGRRGFYSSFQYVTLVA
GQMVALGVQIVLQQLLSEPDMKAWGWRIPFIIGAMGAVAVLWLRRTMDESEQFSNIKSQKRESAGTVRALMKHPKAVLTV
VGLTLGGTVAFYTYTTYLQKFMVNTVGLPKEVVSWINFVALLIFVVLQPIAGLLSDKIGRRPLLMAFGILGTLLTAPIFF
FMEKTTEPIVAFLLMMVGLIIVTGYTSINAIVKAELFPTEIRALGVGLPYALTVAIFGGTAEFIALWLKSIGMESLFYFY
VAGCIAISFITYWRMDESSKTSQIEAELGGGDTLVNNKSS
>Mature_439_residues
AQAKSNRVAGNIFKGSVGNLIEWYDWYVYSAFAVYFSAEFFPKGDPTSQLLNTAAIFAVGFLMRPIGSLLMGRYADRHGR
RAALTLSITVMAGGSFIIACTPSYESIGIMAPIILVLARLLQGLSLGGEYGTSATYLSEMASSGRRGFYSSFQYVTLVAG
QMVALGVQIVLQQLLSEPDMKAWGWRIPFIIGAMGAVAVLWLRRTMDESEQFSNIKSQKRESAGTVRALMKHPKAVLTVV
GLTLGGTVAFYTYTTYLQKFMVNTVGLPKEVVSWINFVALLIFVVLQPIAGLLSDKIGRRPLLMAFGILGTLLTAPIFFF
MEKTTEPIVAFLLMMVGLIIVTGYTSINAIVKAELFPTEIRALGVGLPYALTVAIFGGTAEFIALWLKSIGMESLFYFYV
AGCIAISFITYWRMDESSKTSQIEAELGGGDTLVNNKSS

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=420, Percent_Identity=50, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=28.2710280373832, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788292, Length=427, Percent_Identity=29.7423887587822, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789941, Length=404, Percent_Identity=33.9108910891089, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI87082231, Length=255, Percent_Identity=27.0588235294118, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082404, Length=342, Percent_Identity=24.5614035087719, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48008; Mature: 47877

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAKSNRVAGNIFKGSVGNLIEWYDWYVYSAFAVYFSAEFFPKGDPTSQLLNTAAIFAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GFLMRPIGSLLMGRYADRHGRRAALTLSITVMAGGSFIIACTPSYESIGIMAPIILVLAR
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHH
LLQGLSLGGEYGTSATYLSEMASSGRRGFYSSFQYVTLVAGQMVALGVQIVLQQLLSEPD
HHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MKAWGWRIPFIIGAMGAVAVLWLRRTMDESEQFSNIKSQKRESAGTVRALMKHPKAVLTV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VGLTLGGTVAFYTYTTYLQKFMVNTVGLPKEVVSWINFVALLIFVVLQPIAGLLSDKIGR
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPLLMAFGILGTLLTAPIFFFMEKTTEPIVAFLLMMVGLIIVTGYTSINAIVKAELFPTE
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
IRALGVGLPYALTVAIFGGTAEFIALWLKSIGMESLFYFYVAGCIAISFITYWRMDESSK
HHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TSQIEAELGGGDTLVNNKSS
CCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AQAKSNRVAGNIFKGSVGNLIEWYDWYVYSAFAVYFSAEFFPKGDPTSQLLNTAAIFAV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GFLMRPIGSLLMGRYADRHGRRAALTLSITVMAGGSFIIACTPSYESIGIMAPIILVLAR
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHH
LLQGLSLGGEYGTSATYLSEMASSGRRGFYSSFQYVTLVAGQMVALGVQIVLQQLLSEPD
HHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MKAWGWRIPFIIGAMGAVAVLWLRRTMDESEQFSNIKSQKRESAGTVRALMKHPKAVLTV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VGLTLGGTVAFYTYTTYLQKFMVNTVGLPKEVVSWINFVALLIFVVLQPIAGLLSDKIGR
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPLLMAFGILGTLLTAPIFFFMEKTTEPIVAFLLMMVGLIIVTGYTSINAIVKAELFPTE
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
IRALGVGLPYALTVAIFGGTAEFIALWLKSIGMESLFYFYVAGCIAISFITYWRMDESSK
HHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TSQIEAELGGGDTLVNNKSS
CCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]